Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Löwenstein-Jensen and TK-SLC medium
Background: Mycobacterial antigen MPB64 is a secretory protein specific for Mycobacterium tuberculosis complex. A lateral flow immunochromatographic assay (ICA) is a method used for the rapid differentiation of M. tuberculosis complex. Aim: We aimed to evaluate the performance of ICA in rapid differ...
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| creator | Akyar, I Kocagoz, T Sinik, G Oktem, S Aytekin, N Kocagoz, S |
| description | Background: Mycobacterial antigen MPB64 is a secretory protein specific
for Mycobacterium tuberculosis complex. A lateral flow
immunochromatographic assay (ICA) is a method used for the rapid
differentiation of M. tuberculosis complex. Aim: We aimed to evaluate
the performance of ICA in rapid differentiation of M. tuberculosis
complex from 97 Mycobacterium species other than tuberculosis (MOTT),
which are grown in Lφwenstein-Jensen and TK-selective (SLC)
medium. Materials and Methods: The study was performed in our
laboratory between January 2009 and January 2010. A total of 394
isolates consisting of reference strains of 34 M. tuberculosis from
World Health Organization (WHO) collection, 97 different MOTT bacilli,
7 Mycobacterium bovis BCG substrains and total 256 clinical
Mycobacterium isolates were tested by ICA, which is based on anti-MPB64
monoclonal antibodies. All the strains were inoculated onto a TK-SLC
(selective) medium and Lφwenstein-Jensen medium. TK-SLC is a new
rapid mycobacterial culture medium that indicates mycobacterial growth
by colour change. Results: The growth of mycobacterial strains was
observed in 10-12 days on TK-SLC medium. ICA test was performed in 15
minutes. All strains belonging to M. tuberculosis complex group were
found positive and all MOTT species were found negative on ICA slides.
The results were confirmed with nucleic acid amplification by
polymerase chain reaction (PCR) using primers specific for M.
tuberculosis complex. Conclusion: With the additive effect of growth on
TK-SLC medium in 10-12 days, the mycobacterial antigen MPB64 is a very
useful and specific tool in rapid differentiation of M. tuberculosis
and MOTT grown in culture. |
| doi_str_mv | 10.4103/0255-0857.71817 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_762315550</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S025508572101330X</els_id><sourcerecordid>2179794001</sourcerecordid><originalsourceid>FETCH-LOGICAL-b383t-9b2c9368c4c1c6824084fd025c60d8169b5d10abe1397ad31edc04594832bdc83</originalsourceid><addsrcrecordid>eNp9UUtv1DAYtBCILoUzN2Rxz9aOE8c-ohXvIA6Us-XHF-oqsRc76bJ_DH4Afwxvt13EBcmSXzPzaWYQek7JuqGEXZC6bSsi2m7dUUG7B2hFpRQV4w1_iFan3zP0JOdrUu6NbB6js5pIzltOVuhXr2dIesTDGHdY56z3eIgJJ731Djs_DJAgzF7PPgYcB_xpb6PRtpD8MuF5MZDsMsbsM7Zx2o7wA-vgsOxw3oL1kA-k6S9J4zhfQcLzlQ7_0r-luAvYB9z__rmDkGfwofpQDhBuJS8_Vl_6DZ7AlclP0aNBjxme3e3n6Oub15ebd1X_-e37zau-MkywuZKmtpJxYRtLLRd1Q0QzuJKD5cQJyqVpHSXaAGWy045RcJY0rWwEq42zgp2jl0fdbYrfF8izuo5LCmWk6njNaNu2pIAujiCbYs4JBrVNftJpryhRh57UoQl1aELd9lQYL-5kF1MMnfD3xRSAPAKgmLvxkFQuYQZbzCews3LR_0d8feQaH0cf4KRuS_zq_nEyZVFSBrI_64y0vQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>762315550</pqid></control><display><type>article</type><title>Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Löwenstein-Jensen and TK-SLC medium</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Bioline International</source><source>ProQuest Central UK/Ireland</source><source>Alma/SFX Local Collection</source><creator>Akyar, I ; Kocagoz, T ; Sinik, G ; Oktem, S ; Aytekin, N ; Kocagoz, S</creator><creatorcontrib>Akyar, I ; Kocagoz, T ; Sinik, G ; Oktem, S ; Aytekin, N ; Kocagoz, S</creatorcontrib><description>Background: Mycobacterial antigen MPB64 is a secretory protein specific
for Mycobacterium tuberculosis complex. A lateral flow
immunochromatographic assay (ICA) is a method used for the rapid
differentiation of M. tuberculosis complex. Aim: We aimed to evaluate
the performance of ICA in rapid differentiation of M. tuberculosis
complex from 97 Mycobacterium species other than tuberculosis (MOTT),
which are grown in Lφwenstein-Jensen and TK-selective (SLC)
medium. Materials and Methods: The study was performed in our
laboratory between January 2009 and January 2010. A total of 394
isolates consisting of reference strains of 34 M. tuberculosis from
World Health Organization (WHO) collection, 97 different MOTT bacilli,
7 Mycobacterium bovis BCG substrains and total 256 clinical
Mycobacterium isolates were tested by ICA, which is based on anti-MPB64
monoclonal antibodies. All the strains were inoculated onto a TK-SLC
(selective) medium and Lφwenstein-Jensen medium. TK-SLC is a new
rapid mycobacterial culture medium that indicates mycobacterial growth
by colour change. Results: The growth of mycobacterial strains was
observed in 10-12 days on TK-SLC medium. ICA test was performed in 15
minutes. All strains belonging to M. tuberculosis complex group were
found positive and all MOTT species were found negative on ICA slides.
The results were confirmed with nucleic acid amplification by
polymerase chain reaction (PCR) using primers specific for M.
tuberculosis complex. Conclusion: With the additive effect of growth on
TK-SLC medium in 10-12 days, the mycobacterial antigen MPB64 is a very
useful and specific tool in rapid differentiation of M. tuberculosis
and MOTT grown in culture.</description><identifier>ISSN: 0255-0857</identifier><identifier>EISSN: 1998-3646</identifier><identifier>DOI: 10.4103/0255-0857.71817</identifier><identifier>PMID: 20966560</identifier><language>eng</language><publisher>India: Medknow Publications on behalf of Indian Association of Medical Microbiology</publisher><subject>Acids ; Antibodies, Bacterial - immunology ; Antibodies, Monoclonal - immunology ; Antigens, Bacterial - immunology ; Atypic mycobacteria ; Atypic mycobacteria, MPB64 protein, Mycobacterium tuberculosis, mycobacterium ; Bacteria ; Bacterial Proteins - immunology ; Bacterial Typing Techniques ; Chromatography - methods ; Culture Media ; Disease ; Humans ; Methods ; Microbiology ; MPB64 protein ; mycobacterium ; Mycobacterium - classification ; Mycobacterium - growth & development ; Mycobacterium - immunology ; Mycobacterium Infections - diagnosis ; Mycobacterium Infections - microbiology ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - classification ; Mycobacterium tuberculosis - growth & development ; Mycobacterium tuberculosis - immunology ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Species Specificity ; Time Factors ; Tuberculosis ; Tuberculosis - diagnosis ; Tuberculosis - microbiology</subject><ispartof>Indian journal of medical microbiology, 2010-10, Vol.28 (4), p.308-312</ispartof><rights>Copyright 2010 Indian Journal of Medical Microbiology.</rights><rights>2010 Indian Journal of Medical Microbiology</rights><rights>Copyright Medknow Publications & Media Pvt. Ltd. Oct 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b383t-9b2c9368c4c1c6824084fd025c60d8169b5d10abe1397ad31edc04594832bdc83</citedby><cites>FETCH-LOGICAL-b383t-9b2c9368c4c1c6824084fd025c60d8169b5d10abe1397ad31edc04594832bdc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/762315550?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27903,27904,64362,64366,72216,79173</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20966560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akyar, I</creatorcontrib><creatorcontrib>Kocagoz, T</creatorcontrib><creatorcontrib>Sinik, G</creatorcontrib><creatorcontrib>Oktem, S</creatorcontrib><creatorcontrib>Aytekin, N</creatorcontrib><creatorcontrib>Kocagoz, S</creatorcontrib><title>Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Löwenstein-Jensen and TK-SLC medium</title><title>Indian journal of medical microbiology</title><addtitle>Indian J Med Microbiol</addtitle><description>Background: Mycobacterial antigen MPB64 is a secretory protein specific
for Mycobacterium tuberculosis complex. A lateral flow
immunochromatographic assay (ICA) is a method used for the rapid
differentiation of M. tuberculosis complex. Aim: We aimed to evaluate
the performance of ICA in rapid differentiation of M. tuberculosis
complex from 97 Mycobacterium species other than tuberculosis (MOTT),
which are grown in Lφwenstein-Jensen and TK-selective (SLC)
medium. Materials and Methods: The study was performed in our
laboratory between January 2009 and January 2010. A total of 394
isolates consisting of reference strains of 34 M. tuberculosis from
World Health Organization (WHO) collection, 97 different MOTT bacilli,
7 Mycobacterium bovis BCG substrains and total 256 clinical
Mycobacterium isolates were tested by ICA, which is based on anti-MPB64
monoclonal antibodies. All the strains were inoculated onto a TK-SLC
(selective) medium and Lφwenstein-Jensen medium. TK-SLC is a new
rapid mycobacterial culture medium that indicates mycobacterial growth
by colour change. Results: The growth of mycobacterial strains was
observed in 10-12 days on TK-SLC medium. ICA test was performed in 15
minutes. All strains belonging to M. tuberculosis complex group were
found positive and all MOTT species were found negative on ICA slides.
The results were confirmed with nucleic acid amplification by
polymerase chain reaction (PCR) using primers specific for M.
tuberculosis complex. Conclusion: With the additive effect of growth on
TK-SLC medium in 10-12 days, the mycobacterial antigen MPB64 is a very
useful and specific tool in rapid differentiation of M. tuberculosis
and MOTT grown in culture.</description><subject>Acids</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Bacterial - immunology</subject><subject>Atypic mycobacteria</subject><subject>Atypic mycobacteria, MPB64 protein, Mycobacterium tuberculosis, mycobacterium</subject><subject>Bacteria</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Typing Techniques</subject><subject>Chromatography - methods</subject><subject>Culture Media</subject><subject>Disease</subject><subject>Humans</subject><subject>Methods</subject><subject>Microbiology</subject><subject>MPB64 protein</subject><subject>mycobacterium</subject><subject>Mycobacterium - classification</subject><subject>Mycobacterium - growth & development</subject><subject>Mycobacterium - immunology</subject><subject>Mycobacterium Infections - diagnosis</subject><subject>Mycobacterium Infections - microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - classification</subject><subject>Mycobacterium tuberculosis - growth & development</subject><subject>Mycobacterium tuberculosis - immunology</subject><subject>Polymerase Chain Reaction</subject><subject>Sensitivity and Specificity</subject><subject>Species Specificity</subject><subject>Time Factors</subject><subject>Tuberculosis</subject><subject>Tuberculosis - diagnosis</subject><subject>Tuberculosis - microbiology</subject><issn>0255-0857</issn><issn>1998-3646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9UUtv1DAYtBCILoUzN2Rxz9aOE8c-ohXvIA6Us-XHF-oqsRc76bJ_DH4Afwxvt13EBcmSXzPzaWYQek7JuqGEXZC6bSsi2m7dUUG7B2hFpRQV4w1_iFan3zP0JOdrUu6NbB6js5pIzltOVuhXr2dIesTDGHdY56z3eIgJJ731Djs_DJAgzF7PPgYcB_xpb6PRtpD8MuF5MZDsMsbsM7Zx2o7wA-vgsOxw3oL1kA-k6S9J4zhfQcLzlQ7_0r-luAvYB9z__rmDkGfwofpQDhBuJS8_Vl_6DZ7AlclP0aNBjxme3e3n6Oub15ebd1X_-e37zau-MkywuZKmtpJxYRtLLRd1Q0QzuJKD5cQJyqVpHSXaAGWy045RcJY0rWwEq42zgp2jl0fdbYrfF8izuo5LCmWk6njNaNu2pIAujiCbYs4JBrVNftJpryhRh57UoQl1aELd9lQYL-5kF1MMnfD3xRSAPAKgmLvxkFQuYQZbzCews3LR_0d8feQaH0cf4KRuS_zq_nEyZVFSBrI_64y0vQ</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Akyar, I</creator><creator>Kocagoz, T</creator><creator>Sinik, G</creator><creator>Oktem, S</creator><creator>Aytekin, N</creator><creator>Kocagoz, S</creator><general>Medknow Publications on behalf of Indian Association of Medical Microbiology</general><general>Elsevier B.V</general><general>Elsevier Limited</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20101001</creationdate><title>Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Löwenstein-Jensen and TK-SLC medium</title><author>Akyar, I ; Kocagoz, T ; Sinik, G ; Oktem, S ; Aytekin, N ; Kocagoz, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b383t-9b2c9368c4c1c6824084fd025c60d8169b5d10abe1397ad31edc04594832bdc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acids</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Bacterial - immunology</topic><topic>Atypic mycobacteria</topic><topic>Atypic mycobacteria, MPB64 protein, Mycobacterium tuberculosis, mycobacterium</topic><topic>Bacteria</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Typing Techniques</topic><topic>Chromatography - methods</topic><topic>Culture Media</topic><topic>Disease</topic><topic>Humans</topic><topic>Methods</topic><topic>Microbiology</topic><topic>MPB64 protein</topic><topic>mycobacterium</topic><topic>Mycobacterium - classification</topic><topic>Mycobacterium - growth & development</topic><topic>Mycobacterium - immunology</topic><topic>Mycobacterium Infections - diagnosis</topic><topic>Mycobacterium Infections - microbiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - classification</topic><topic>Mycobacterium tuberculosis - growth & development</topic><topic>Mycobacterium tuberculosis - immunology</topic><topic>Polymerase Chain Reaction</topic><topic>Sensitivity and Specificity</topic><topic>Species Specificity</topic><topic>Time Factors</topic><topic>Tuberculosis</topic><topic>Tuberculosis - diagnosis</topic><topic>Tuberculosis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akyar, I</creatorcontrib><creatorcontrib>Kocagoz, T</creatorcontrib><creatorcontrib>Sinik, G</creatorcontrib><creatorcontrib>Oktem, S</creatorcontrib><creatorcontrib>Aytekin, N</creatorcontrib><creatorcontrib>Kocagoz, S</creatorcontrib><collection>Bioline International</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Indian journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akyar, I</au><au>Kocagoz, T</au><au>Sinik, G</au><au>Oktem, S</au><au>Aytekin, N</au><au>Kocagoz, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Löwenstein-Jensen and TK-SLC medium</atitle><jtitle>Indian journal of medical microbiology</jtitle><addtitle>Indian J Med Microbiol</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>28</volume><issue>4</issue><spage>308</spage><epage>312</epage><pages>308-312</pages><issn>0255-0857</issn><eissn>1998-3646</eissn><abstract>Background: Mycobacterial antigen MPB64 is a secretory protein specific
for Mycobacterium tuberculosis complex. A lateral flow
immunochromatographic assay (ICA) is a method used for the rapid
differentiation of M. tuberculosis complex. Aim: We aimed to evaluate
the performance of ICA in rapid differentiation of M. tuberculosis
complex from 97 Mycobacterium species other than tuberculosis (MOTT),
which are grown in Lφwenstein-Jensen and TK-selective (SLC)
medium. Materials and Methods: The study was performed in our
laboratory between January 2009 and January 2010. A total of 394
isolates consisting of reference strains of 34 M. tuberculosis from
World Health Organization (WHO) collection, 97 different MOTT bacilli,
7 Mycobacterium bovis BCG substrains and total 256 clinical
Mycobacterium isolates were tested by ICA, which is based on anti-MPB64
monoclonal antibodies. All the strains were inoculated onto a TK-SLC
(selective) medium and Lφwenstein-Jensen medium. TK-SLC is a new
rapid mycobacterial culture medium that indicates mycobacterial growth
by colour change. Results: The growth of mycobacterial strains was
observed in 10-12 days on TK-SLC medium. ICA test was performed in 15
minutes. All strains belonging to M. tuberculosis complex group were
found positive and all MOTT species were found negative on ICA slides.
The results were confirmed with nucleic acid amplification by
polymerase chain reaction (PCR) using primers specific for M.
tuberculosis complex. Conclusion: With the additive effect of growth on
TK-SLC medium in 10-12 days, the mycobacterial antigen MPB64 is a very
useful and specific tool in rapid differentiation of M. tuberculosis
and MOTT grown in culture.</abstract><cop>India</cop><pub>Medknow Publications on behalf of Indian Association of Medical Microbiology</pub><pmid>20966560</pmid><doi>10.4103/0255-0857.71817</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0255-0857 |
| ispartof | Indian journal of medical microbiology, 2010-10, Vol.28 (4), p.308-312 |
| issn | 0255-0857 1998-3646 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Bioline International; ProQuest Central UK/Ireland; Alma/SFX Local Collection |
| subjects | Acids Antibodies, Bacterial - immunology Antibodies, Monoclonal - immunology Antigens, Bacterial - immunology Atypic mycobacteria Atypic mycobacteria, MPB64 protein, Mycobacterium tuberculosis, mycobacterium Bacteria Bacterial Proteins - immunology Bacterial Typing Techniques Chromatography - methods Culture Media Disease Humans Methods Microbiology MPB64 protein mycobacterium Mycobacterium - classification Mycobacterium - growth & development Mycobacterium - immunology Mycobacterium Infections - diagnosis Mycobacterium Infections - microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - immunology Polymerase Chain Reaction Sensitivity and Specificity Species Specificity Time Factors Tuberculosis Tuberculosis - diagnosis Tuberculosis - microbiology |
| title | Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Löwenstein-Jensen and TK-SLC medium |
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