Complete genome sequence and analysis of theStreptomyces aureofaciens phage μ1/6

The entire double-stranded DNA genome of theStreptomyces aureofaciens phage μ1/6 was sequenced and analyzed. Its size is 38.194 kbp with an overall molar G+C content of 71.2 % Fifty-two potential open reading frames (orfs) were identified, divided into two oppositely transcribed regions. In the left arm of the μ1/6 genome, an identified putative integrase and possible regulation proteins were identified. The rightwards transcribed region contains genes organized into apparently four functional units responsible for: (i) replication, (ii) DNA packaging and head assembly, (iii) tail morphogenesis, and (iv) lysis. Putative functions were assigned to twelveorfs based on bioinformatic analysis or experimental substantiation. Comparative analysis with three complete genomes of streptomycete phages revealed resemblance with respect to the organization of their genes into functional modules. Closer relationship was observed only between μ1/6 andS. venezuelae phage VWB.