A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR
SummaryA test procedure for PSTVd is described based on immobilisation of plant sap on filter paper, by dotting or tissue printing followed by RT-PCR. Tests were carried out using primarily and secondarily infected potato plants, primarily infected in vitro plants, and potato tubers. Print PCR was s...
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| Veröffentlicht in: | Potato research 1998-03, Vol.41 (1), p.1-8 |
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| container_title | Potato research |
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| creator | WEIDEMANN, H.-L BUCHTA, U |
| description | SummaryA test procedure for PSTVd is described based on immobilisation of plant sap on filter paper, by dotting or tissue printing followed by RT-PCR. Tests were carried out using primarily and secondarily infected potato plants, primarily infected in vitro plants, and potato tubers. Print PCR was shown to be suitable for testing large samples of potato plants whereas dot PCR is recommended for in vitro plantlets and tuber tissue. Bulking one infected plant to 4 or 9 healthy plants gave reliable results with secondarily infected potato plants, but sometimes the test failed to detect PSTVd in primarily infected in vitro plants. Dotted and printed paper squares could be stored at 4°C for at least 2 weeks in Triton X-100 solution or under dry conditions. Storing at room temperature can lead to unreliable results. |
| doi_str_mv | 10.1007/bf02360256 |
| format | Article |
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Tests were carried out using primarily and secondarily infected potato plants, primarily infected in vitro plants, and potato tubers. Print PCR was shown to be suitable for testing large samples of potato plants whereas dot PCR is recommended for in vitro plantlets and tuber tissue. Bulking one infected plant to 4 or 9 healthy plants gave reliable results with secondarily infected potato plants, but sometimes the test failed to detect PSTVd in primarily infected in vitro plants. Dotted and printed paper squares could be stored at 4°C for at least 2 weeks in Triton X-100 solution or under dry conditions. Storing at room temperature can lead to unreliable results.</description><identifier>ISSN: 0014-3065</identifier><identifier>EISSN: 1871-4528</identifier><identifier>DOI: 10.1007/bf02360256</identifier><identifier>CODEN: PORHBW</identifier><language>eng</language><publisher>Wageningen: European Association for Potato Research</publisher><subject>Biological and medical sciences ; Biotechnology ; Filter paper ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; Immobilization ; Microbiology ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; Plantlets ; Polymerase chain reaction ; Potatoes ; Room temperature ; Techniques used in virology ; Test procedures ; Tubers ; Vegetables ; Virology</subject><ispartof>Potato research, 1998-03, Vol.41 (1), p.1-8</ispartof><rights>1999 INIST-CNRS</rights><rights>Kluwer Academic Publishers 1998.</rights><rights>Kluwer Academic Publishers 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-3235959bcc70584e139ed0f2dbf7da1585c3a17139f090c39e1400f36831a6d73</citedby><cites>FETCH-LOGICAL-c379t-3235959bcc70584e139ed0f2dbf7da1585c3a17139f090c39e1400f36831a6d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1710743$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>WEIDEMANN, H.-L</creatorcontrib><creatorcontrib>BUCHTA, U</creatorcontrib><title>A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR</title><title>Potato research</title><description>SummaryA test procedure for PSTVd is described based on immobilisation of plant sap on filter paper, by dotting or tissue printing followed by RT-PCR. Tests were carried out using primarily and secondarily infected potato plants, primarily infected in vitro plants, and potato tubers. Print PCR was shown to be suitable for testing large samples of potato plants whereas dot PCR is recommended for in vitro plantlets and tuber tissue. Bulking one infected plant to 4 or 9 healthy plants gave reliable results with secondarily infected potato plants, but sometimes the test failed to detect PSTVd in primarily infected in vitro plants. Dotted and printed paper squares could be stored at 4°C for at least 2 weeks in Triton X-100 solution or under dry conditions. Storing at room temperature can lead to unreliable results.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Filter paper</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>Immobilization</subject><subject>Microbiology</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>Plantlets</subject><subject>Polymerase chain reaction</subject><subject>Potatoes</subject><subject>Room temperature</subject><subject>Techniques used in virology</subject><subject>Test procedures</subject><subject>Tubers</subject><subject>Vegetables</subject><subject>Virology</subject><issn>0014-3065</issn><issn>1871-4528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kEtLxDAUhYMoOI5u_AVBXahQvbdpmnapgy8QlHF0JZQ0D6bDTFOTjOC_N6LiytWFc79zDhxC9hHOEECctxZyVkLOyw0ywkpgVvC82iQjACwyBiXfJjshLAAKzpGPyOsFDd1qWBoqe029HDpNVybOnabWeRrnhmoTjYqd66mzdHBRRkfD0PU6meK6NZ6-d94l3_Hj0-xFn9D2g05n2eNkuku2rFwGs_dzx-T5-mo2uc3uH27uJhf3mWKijhnLGa953SolgFeFQVYbDTbXrRVaIq-4YhJFki3UoNIXCwDLyoqhLLVgY3LwnTt497Y2ITYLt_Z9qmwEL5AjCEjQ4X9QXtYcMK-QJer0m1LeheCNbQbfraT_aBCar4mby-vfiRN89BMpg5JL62WvuvDnEKm4YOwT-9t24g</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>WEIDEMANN, H.-L</creator><creator>BUCHTA, U</creator><general>European Association for Potato Research</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>19980301</creationdate><title>A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR</title><author>WEIDEMANN, H.-L ; BUCHTA, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-3235959bcc70584e139ed0f2dbf7da1585c3a17139f090c39e1400f36831a6d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Filter paper</topic><topic>Fundamental and applied biological sciences. 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Tests were carried out using primarily and secondarily infected potato plants, primarily infected in vitro plants, and potato tubers. Print PCR was shown to be suitable for testing large samples of potato plants whereas dot PCR is recommended for in vitro plantlets and tuber tissue. Bulking one infected plant to 4 or 9 healthy plants gave reliable results with secondarily infected potato plants, but sometimes the test failed to detect PSTVd in primarily infected in vitro plants. Dotted and printed paper squares could be stored at 4°C for at least 2 weeks in Triton X-100 solution or under dry conditions. Storing at room temperature can lead to unreliable results.</abstract><cop>Wageningen</cop><pub>European Association for Potato Research</pub><doi>10.1007/bf02360256</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Biotechnology Filter paper Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Immobilization Microbiology Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids Plantlets Polymerase chain reaction Potatoes Room temperature Techniques used in virology Test procedures Tubers Vegetables Virology |
| title | A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR |
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