Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. 'Baron') are not apomictic in origin
Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a self-pollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embry...
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| Veröffentlicht in: | Euphytica 2006-02, Vol.147 (3), p.383-388 |
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| creator | Stephens, L.C Fei, S.Z Xiong, Y Hodges, C.F |
| description | Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a self-pollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embryos that were plated. Flow-cytometric (FCM) and RAPD-marker analyses were performed to determine if regenerants were or were not apomictic in origin. Fifteen regenerants with a 3c DNA content were classified as arising from 2n + n aberrant embryos, which was a higher frequency than expected, based on a chi-square analysis. Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed. |
| doi_str_mv | 10.1007/s10681-005-9034-y |
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Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed.</description><identifier>ISSN: 0014-2336</identifier><identifier>EISSN: 1573-5060</identifier><identifier>DOI: 10.1007/s10681-005-9034-y</identifier><identifier>CODEN: EUPHAA</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agronomy. Soil science and plant productions ; apomixis ; Biological and medical sciences ; Crop science ; Cultivars ; Deoxyribonucleic acid ; DNA ; embryo (plant) ; embryo culture ; Embryos ; flow cytometry ; Fundamental and applied biological sciences. 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'Baron') are not apomictic in origin</title><title>Euphytica</title><description>Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a self-pollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embryos that were plated. Flow-cytometric (FCM) and RAPD-marker analyses were performed to determine if regenerants were or were not apomictic in origin. Fifteen regenerants with a 3c DNA content were classified as arising from 2n + n aberrant embryos, which was a higher frequency than expected, based on a chi-square analysis. Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed.</description><subject>Agronomy. Soil science and plant productions</subject><subject>apomixis</subject><subject>Biological and medical sciences</subject><subject>Crop science</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>embryo (plant)</subject><subject>embryo culture</subject><subject>Embryos</subject><subject>flow cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic markers</subject><subject>Genetics and breeding of economic plants</subject><subject>Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia</subject><subject>in vitro regeneration</subject><subject>Phenotypes</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Poa pratensis</subject><subject>random amplified polymorphic DNA technique</subject><subject>tissue culture</subject><subject>turf grasses</subject><subject>Turfgrasses</subject><issn>0014-2336</issn><issn>1573-5060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpNkE1r3DAQhkVpIdu0PyCnikJJe3A6Y0n-OKahbUIWEkhyFrNaaXHqlbYj--C_0F9dmw20p4HhfZ9hHiHOEC4QoP6aEaoGCwBTtKB0Mb0SKzS1KgxU8FqsAFAXpVLViXib8zMAtLWBlfhz31McsmS_89EzDX4rA6e99PsNT0m6sR9G9lmmIClKOqR954bOSden6JftrY_D6H5NctOPfseUs_x8n0geFljMXZbrC3n-jTjF8y-S2MuYhv9AXZSJu10X34k3gfrs37_MU_H04_vj1XWxvvt5c3W5Lpwqq6HAtsGK3MbrirBSCNqExviWvG6UK8GBc60ONSgzv0iGwjaAW1xsQ20UqFPx8cg9cPo9-jzY5zRynE_aWiNig1rPITyGHKec2Qd74G5PPFkEuxi3R-N2Nm4X43aaO59ewJQd9YEpui7_K9YNlrpZ2B-OuUDJ0o7nzNNDCagAodGmBPUXYRuKng</recordid><startdate>200602</startdate><enddate>200602</enddate><creator>Stephens, L.C</creator><creator>Fei, S.Z</creator><creator>Xiong, Y</creator><creator>Hodges, C.F</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TM</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>200602</creationdate><title>Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. 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Floral biology applications: apomixy, male sterility, incompatibility, varia</topic><topic>in vitro regeneration</topic><topic>Phenotypes</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Poa pratensis</topic><topic>random amplified polymorphic DNA technique</topic><topic>tissue culture</topic><topic>turf grasses</topic><topic>Turfgrasses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stephens, L.C</creatorcontrib><creatorcontrib>Fei, S.Z</creatorcontrib><creatorcontrib>Xiong, Y</creatorcontrib><creatorcontrib>Hodges, C.F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Euphytica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stephens, L.C</au><au>Fei, S.Z</au><au>Xiong, Y</au><au>Hodges, C.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. 'Baron') are not apomictic in origin</atitle><jtitle>Euphytica</jtitle><date>2006-02</date><risdate>2006</risdate><volume>147</volume><issue>3</issue><spage>383</spage><epage>388</epage><pages>383-388</pages><issn>0014-2336</issn><eissn>1573-5060</eissn><coden>EUPHAA</coden><abstract>Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a self-pollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embryos that were plated. Flow-cytometric (FCM) and RAPD-marker analyses were performed to determine if regenerants were or were not apomictic in origin. Fifteen regenerants with a 3c DNA content were classified as arising from 2n + n aberrant embryos, which was a higher frequency than expected, based on a chi-square analysis. Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/s10681-005-9034-y</doi><tpages>6</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions apomixis Biological and medical sciences Crop science Cultivars Deoxyribonucleic acid DNA embryo (plant) embryo culture Embryos flow cytometry Fundamental and applied biological sciences. Psychology genetic markers Genetics and breeding of economic plants Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia in vitro regeneration Phenotypes Plant breeding: fundamental aspects and methodology Poa pratensis random amplified polymorphic DNA technique tissue culture turf grasses Turfgrasses |
| title | Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. 'Baron') are not apomictic in origin |
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