Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity

So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biotechnology letters 2007-12, Vol.29 (12), p.1811-1816
Hauptverfasser: Wang, Yugang, Guo, Leiming, Zhao, Kunpeng, Chen, Jugao, Feng, Jiannan, Sun, Yingxun, Li, Yan, Shen, Beifen
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1816
container_issue 12
container_start_page 1811
container_title Biotechnology letters
container_volume 29
creator Wang, Yugang
Guo, Leiming
Zhao, Kunpeng
Chen, Jugao
Feng, Jiannan
Sun, Yingxun
Li, Yan
Shen, Beifen
description So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.
doi_str_mv 10.1007/s10529-007-9478-3
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_734864250</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2100630501</sourcerecordid><originalsourceid>FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</originalsourceid><addsrcrecordid>eNpFkE9PGzEQxa2qVQmhH6CXdlWJo8v4__pYRRSQInqAXHqxbMcbjJJdajuh4dPjsJE4zTv83puZh9BXAj8JgLrIBATVuEqsuWox-4AmRCiGpVLyI5oA4QQLrukJOs35EQC0AvUZnRAlheKETdDf22EX1o1_iJuQom9sXyKuIvZv0g3LfTPjM8Ka51gemj5sS7Lr-BL7VWN9ibtY9o1d2djn0oy-Mvyvs-zP0KfOrnP4cpxTtPh9eT-7xvM_VzezX3PsWQsFa0etlE4o7ZXggjstGBOtk7TVmihNpZWeK0GY7Or1wXWKtc62ywCOa-Bsin6MuU9p-LcNuZjHYZv6utIoxlvJqYAKkRHyacg5hc48pbixaW8ImEOZZizTHOShTMOq59sxeOs2YfnuOLZXgfMjYLO36y7Z3sf8zmmmmBS6ct9HrrODsatUmcUdBcIAWkppffgVHHCEbA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734864250</pqid></control><display><type>article</type><title>Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Wang, Yugang ; Guo, Leiming ; Zhao, Kunpeng ; Chen, Jugao ; Feng, Jiannan ; Sun, Yingxun ; Li, Yan ; Shen, Beifen</creator><creatorcontrib>Wang, Yugang ; Guo, Leiming ; Zhao, Kunpeng ; Chen, Jugao ; Feng, Jiannan ; Sun, Yingxun ; Li, Yan ; Shen, Beifen</creatorcontrib><description>So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-007-9478-3</identifier><identifier>PMID: 17657413</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Amino Acid Sequence ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; Antibody Specificity - drug effects ; Base Sequence ; Binding Sites, Antibody ; Binding, Competitive - drug effects ; Biological and medical sciences ; Biotechnology ; Blotting, Western ; Cell Death - drug effects ; Chimeric antibody ; Cytotoxicity ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoglobulin Variable Region - chemistry ; Jurkat Cells ; Molecular Sequence Data ; Neutralization Tests ; Neutralizing activity ; Recombinant Proteins - chemistry ; Recombinant Proteins - immunology ; ricin ; Ricin - immunology ; Ricin - toxicity ; Rodents ; toxicity</subject><ispartof>Biotechnology letters, 2007-12, Vol.29 (12), p.1811-1816</ispartof><rights>2008 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</citedby><cites>FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=19373659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17657413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yugang</creatorcontrib><creatorcontrib>Guo, Leiming</creatorcontrib><creatorcontrib>Zhao, Kunpeng</creatorcontrib><creatorcontrib>Chen, Jugao</creatorcontrib><creatorcontrib>Feng, Jiannan</creatorcontrib><creatorcontrib>Sun, Yingxun</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Shen, Beifen</creatorcontrib><title>Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.</description><subject>Amino Acid Sequence</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity - drug effects</subject><subject>Base Sequence</subject><subject>Binding Sites, Antibody</subject><subject>Binding, Competitive - drug effects</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Cell Death - drug effects</subject><subject>Chimeric antibody</subject><subject>Cytotoxicity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoglobulin Variable Region - chemistry</subject><subject>Jurkat Cells</subject><subject>Molecular Sequence Data</subject><subject>Neutralization Tests</subject><subject>Neutralizing activity</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - immunology</subject><subject>ricin</subject><subject>Ricin - immunology</subject><subject>Ricin - toxicity</subject><subject>Rodents</subject><subject>toxicity</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpFkE9PGzEQxa2qVQmhH6CXdlWJo8v4__pYRRSQInqAXHqxbMcbjJJdajuh4dPjsJE4zTv83puZh9BXAj8JgLrIBATVuEqsuWox-4AmRCiGpVLyI5oA4QQLrukJOs35EQC0AvUZnRAlheKETdDf22EX1o1_iJuQom9sXyKuIvZv0g3LfTPjM8Ka51gemj5sS7Lr-BL7VWN9ibtY9o1d2djn0oy-Mvyvs-zP0KfOrnP4cpxTtPh9eT-7xvM_VzezX3PsWQsFa0etlE4o7ZXggjstGBOtk7TVmihNpZWeK0GY7Or1wXWKtc62ywCOa-Bsin6MuU9p-LcNuZjHYZv6utIoxlvJqYAKkRHyacg5hc48pbixaW8ImEOZZizTHOShTMOq59sxeOs2YfnuOLZXgfMjYLO36y7Z3sf8zmmmmBS6ct9HrrODsatUmcUdBcIAWkppffgVHHCEbA</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Wang, Yugang</creator><creator>Guo, Leiming</creator><creator>Zhao, Kunpeng</creator><creator>Chen, Jugao</creator><creator>Feng, Jiannan</creator><creator>Sun, Yingxun</creator><creator>Li, Yan</creator><creator>Shen, Beifen</creator><general>Dordrecht : Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20071201</creationdate><title>Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity</title><author>Wang, Yugang ; Guo, Leiming ; Zhao, Kunpeng ; Chen, Jugao ; Feng, Jiannan ; Sun, Yingxun ; Li, Yan ; Shen, Beifen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity - drug effects</topic><topic>Base Sequence</topic><topic>Binding Sites, Antibody</topic><topic>Binding, Competitive - drug effects</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Cell Death - drug effects</topic><topic>Chimeric antibody</topic><topic>Cytotoxicity</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoglobulin Variable Region - chemistry</topic><topic>Jurkat Cells</topic><topic>Molecular Sequence Data</topic><topic>Neutralization Tests</topic><topic>Neutralizing activity</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - immunology</topic><topic>ricin</topic><topic>Ricin - immunology</topic><topic>Ricin - toxicity</topic><topic>Rodents</topic><topic>toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yugang</creatorcontrib><creatorcontrib>Guo, Leiming</creatorcontrib><creatorcontrib>Zhao, Kunpeng</creatorcontrib><creatorcontrib>Chen, Jugao</creatorcontrib><creatorcontrib>Feng, Jiannan</creatorcontrib><creatorcontrib>Sun, Yingxun</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Shen, Beifen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yugang</au><au>Guo, Leiming</au><au>Zhao, Kunpeng</au><au>Chen, Jugao</au><au>Feng, Jiannan</au><au>Sun, Yingxun</au><au>Li, Yan</au><au>Shen, Beifen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity</atitle><jtitle>Biotechnology letters</jtitle><addtitle>Biotechnol Lett</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>29</volume><issue>12</issue><spage>1811</spage><epage>1816</epage><pages>1811-1816</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>17657413</pmid><doi>10.1007/s10529-007-9478-3</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0141-5492
ispartof Biotechnology letters, 2007-12, Vol.29 (12), p.1811-1816
issn 0141-5492
1573-6776
language eng
recordid cdi_proquest_journals_734864250
source MEDLINE; SpringerNature Journals
subjects Amino Acid Sequence
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
Antibody Specificity - drug effects
Base Sequence
Binding Sites, Antibody
Binding, Competitive - drug effects
Biological and medical sciences
Biotechnology
Blotting, Western
Cell Death - drug effects
Chimeric antibody
Cytotoxicity
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin Variable Region - chemistry
Jurkat Cells
Molecular Sequence Data
Neutralization Tests
Neutralizing activity
Recombinant Proteins - chemistry
Recombinant Proteins - immunology
ricin
Ricin - immunology
Ricin - toxicity
Rodents
toxicity
title Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T20%3A43%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20chimeric%20anti-ricin%20antibody%20C4C13%20with%20neutralizing%20activity%20against%20ricin%20toxicity&rft.jtitle=Biotechnology%20letters&rft.au=Wang,%20Yugang&rft.date=2007-12-01&rft.volume=29&rft.issue=12&rft.spage=1811&rft.epage=1816&rft.pages=1811-1816&rft.issn=0141-5492&rft.eissn=1573-6776&rft.coden=BILED3&rft_id=info:doi/10.1007/s10529-007-9478-3&rft_dat=%3Cproquest_cross%3E2100630501%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=734864250&rft_id=info:pmid/17657413&rfr_iscdi=true