Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity
So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes...
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| Veröffentlicht in: | Biotechnology letters 2007-12, Vol.29 (12), p.1811-1816 |
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| container_issue | 12 |
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| container_title | Biotechnology letters |
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| creator | Wang, Yugang Guo, Leiming Zhao, Kunpeng Chen, Jugao Feng, Jiannan Sun, Yingxun Li, Yan Shen, Beifen |
| description | So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication. |
| doi_str_mv | 10.1007/s10529-007-9478-3 |
| format | Article |
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Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-007-9478-3</identifier><identifier>PMID: 17657413</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Amino Acid Sequence ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; Antibody Specificity - drug effects ; Base Sequence ; Binding Sites, Antibody ; Binding, Competitive - drug effects ; Biological and medical sciences ; Biotechnology ; Blotting, Western ; Cell Death - drug effects ; Chimeric antibody ; Cytotoxicity ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoglobulin Variable Region - chemistry ; Jurkat Cells ; Molecular Sequence Data ; Neutralization Tests ; Neutralizing activity ; Recombinant Proteins - chemistry ; Recombinant Proteins - immunology ; ricin ; Ricin - immunology ; Ricin - toxicity ; Rodents ; toxicity</subject><ispartof>Biotechnology letters, 2007-12, Vol.29 (12), p.1811-1816</ispartof><rights>2008 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</citedby><cites>FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19373659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17657413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yugang</creatorcontrib><creatorcontrib>Guo, Leiming</creatorcontrib><creatorcontrib>Zhao, Kunpeng</creatorcontrib><creatorcontrib>Chen, Jugao</creatorcontrib><creatorcontrib>Feng, Jiannan</creatorcontrib><creatorcontrib>Sun, Yingxun</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Shen, Beifen</creatorcontrib><title>Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>So far, no specific therapeutic agent is available for the treatment of ricin intoxication. Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.</description><subject>Amino Acid Sequence</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity - drug effects</subject><subject>Base Sequence</subject><subject>Binding Sites, Antibody</subject><subject>Binding, Competitive - drug effects</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Cell Death - drug effects</subject><subject>Chimeric antibody</subject><subject>Cytotoxicity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoglobulin Variable Region - chemistry</subject><subject>Jurkat Cells</subject><subject>Molecular Sequence Data</subject><subject>Neutralization Tests</subject><subject>Neutralizing activity</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - immunology</subject><subject>ricin</subject><subject>Ricin - immunology</subject><subject>Ricin - toxicity</subject><subject>Rodents</subject><subject>toxicity</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpFkE9PGzEQxa2qVQmhH6CXdlWJo8v4__pYRRSQInqAXHqxbMcbjJJdajuh4dPjsJE4zTv83puZh9BXAj8JgLrIBATVuEqsuWox-4AmRCiGpVLyI5oA4QQLrukJOs35EQC0AvUZnRAlheKETdDf22EX1o1_iJuQom9sXyKuIvZv0g3LfTPjM8Ka51gemj5sS7Lr-BL7VWN9ibtY9o1d2djn0oy-Mvyvs-zP0KfOrnP4cpxTtPh9eT-7xvM_VzezX3PsWQsFa0etlE4o7ZXggjstGBOtk7TVmihNpZWeK0GY7Or1wXWKtc62ywCOa-Bsin6MuU9p-LcNuZjHYZv6utIoxlvJqYAKkRHyacg5hc48pbixaW8ImEOZZizTHOShTMOq59sxeOs2YfnuOLZXgfMjYLO36y7Z3sf8zmmmmBS6ct9HrrODsatUmcUdBcIAWkppffgVHHCEbA</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Wang, Yugang</creator><creator>Guo, Leiming</creator><creator>Zhao, Kunpeng</creator><creator>Chen, Jugao</creator><creator>Feng, Jiannan</creator><creator>Sun, Yingxun</creator><creator>Li, Yan</creator><creator>Shen, Beifen</creator><general>Dordrecht : Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20071201</creationdate><title>Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity</title><author>Wang, Yugang ; Guo, Leiming ; Zhao, Kunpeng ; Chen, Jugao ; Feng, Jiannan ; Sun, Yingxun ; Li, Yan ; Shen, Beifen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-9b2a66b579c75454b953358b6289917926a6c475136f707ebf738ba8de0b49043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity - drug effects</topic><topic>Base Sequence</topic><topic>Binding Sites, Antibody</topic><topic>Binding, Competitive - drug effects</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Cell Death - drug effects</topic><topic>Chimeric antibody</topic><topic>Cytotoxicity</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoglobulin Variable Region - chemistry</topic><topic>Jurkat Cells</topic><topic>Molecular Sequence Data</topic><topic>Neutralization Tests</topic><topic>Neutralizing activity</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - immunology</topic><topic>ricin</topic><topic>Ricin - immunology</topic><topic>Ricin - toxicity</topic><topic>Rodents</topic><topic>toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yugang</creatorcontrib><creatorcontrib>Guo, Leiming</creatorcontrib><creatorcontrib>Zhao, Kunpeng</creatorcontrib><creatorcontrib>Chen, Jugao</creatorcontrib><creatorcontrib>Feng, Jiannan</creatorcontrib><creatorcontrib>Sun, Yingxun</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Shen, 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Here, VH and VL genes were cloned from a hybridoma cell line secreting anti-ricin mAb 4C13, which could neutralize the toxicity of ricin. A chimeric antibody, c4C13, containing 4C13 mAb variable region genes fused to human constant region genes (gamma 1, kappa), was constructed. C4C13 retained the binding activity and recognized the same, or a closely related, epitope as the original mouse antibody. Furthermore, c4C13 blocked ricin-induced cytotoxicity to SP2/0 cells. Compared with its parental mouse antibody, c4C13 will be safer when used in human body to reverse clinical ricin intoxication.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>17657413</pmid><doi>10.1007/s10529-007-9478-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2007-12, Vol.29 (12), p.1811-1816 |
| issn | 0141-5492 1573-6776 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Amino Acid Sequence Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Antibody Specificity - drug effects Base Sequence Binding Sites, Antibody Binding, Competitive - drug effects Biological and medical sciences Biotechnology Blotting, Western Cell Death - drug effects Chimeric antibody Cytotoxicity Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Humans Immunoglobulin Variable Region - chemistry Jurkat Cells Molecular Sequence Data Neutralization Tests Neutralizing activity Recombinant Proteins - chemistry Recombinant Proteins - immunology ricin Ricin - immunology Ricin - toxicity Rodents toxicity |
| title | Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity |
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