Study of lipid rafts by gel filtration combined with preliminary staining with fluorescently labeled antibodies

Study of lipid rafts by gel filtration combined with preliminary staining with fluorescently labeled antibodies

A novel procedure for detecting raft-associated proteins by gel filtration was developed. Cells were stained with fluorescently labeled protein-specific antibodies, lyzed in a nonionic detergent and gel-filtered on Sepharose 4B. Proteins were identified with the help of fluorescently labeled antibod...

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Veröffentlicht in:Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2007-09, Vol.1 (3), p.219-227
Hauptverfasser: Krutikova, M. P., Krotov, G. I., Zgoda, V. G., Filatov, A. V.
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Sprache:eng
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Immunology
Proteins
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container_title Biochemistry (Moscow). Supplement series A, Membrane and cell biology
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creator Krutikova, M. P.
Krotov, G. I.
Zgoda, V. G.
Filatov, A. V.
description A novel procedure for detecting raft-associated proteins by gel filtration was developed. Cells were stained with fluorescently labeled protein-specific antibodies, lyzed in a nonionic detergent and gel-filtered on Sepharose 4B. Proteins were identified with the help of fluorescently labeled antibodies. Staining of cells with fluorescently labeled antibodies prior to cell lysis significantly simplified the identification procedure. The potentialities of the new approach are demonstrated in the example of several surface proteins associated with lipid rafts either constitutively or in the course of cell activation.[PUBLICATION ABSTRACT]
doi_str_mv 10.1134/S199074780703004X
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1990-7494
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subjects Immunology
Proteins
title Study of lipid rafts by gel filtration combined with preliminary staining with fluorescently labeled antibodies
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