Detection of pea in food by real-time polymerase chain reaction (PCR)

A qualitative 5'-nuclease real-time PCR-based method for the detection of pea (Pisum sativum) in food is described. The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent PCR with pea-specific primers and a TaqMan fluorescent probe. The primers and the prob...

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Veröffentlicht in:	European food research & technology 2006-03, Vol.222 (5-6), p.600-603
Hauptverfasser:	Brežná, B, Hudecová, L, Kuchta, T
Format:	Artikel
Sprache:	eng
Schlagworte:	allergens Biological and medical sciences chloroplast DNA detection exons Food food composition Food industries foods Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General aspects introns Methods of analysis, processing and quality control, regulation, standards Microbiology molecular sequence data Peas polymerase chain reaction protein concentrates quantitative analysis
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