Detection of pea in food by real-time polymerase chain reaction (PCR)
A qualitative 5'-nuclease real-time PCR-based method for the detection of pea (Pisum sativum) in food is described. The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent PCR with pea-specific primers and a TaqMan fluorescent probe. The primers and the prob...
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| Veröffentlicht in: | European food research & technology 2006-03, Vol.222 (5-6), p.600-603 |
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| creator | Brežná, B Hudecová, L Kuchta, T |
| description | A qualitative 5'-nuclease real-time PCR-based method for the detection of pea (Pisum sativum) in food is described. The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent PCR with pea-specific primers and a TaqMan fluorescent probe. The primers and the probe are oriented to the chloroplast DNA intron located between trnL and trnF exons encoding for tRNA. The analytical parameters of the method were inclusivity 100%, exclusivity 100% and the detection limit of 0.11±0.07 ng of pea DNA corresponding to 12±7 diploid pea genome copies. Using a set of model meat pâtés with defined pea contents, a matrix-related detection limit of 0.05% was determined and a linear calibration line was constructed. The presented analytical method was useful for qualitative detection or semiquantitative determination of pea in food products. The method was relatively fast because the analysis could be performed in one working day. |
| doi_str_mv | 10.1007/s00217-005-0168-x |
| format | Article |
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The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent PCR with pea-specific primers and a TaqMan fluorescent probe. The primers and the probe are oriented to the chloroplast DNA intron located between trnL and trnF exons encoding for tRNA. The analytical parameters of the method were inclusivity 100%, exclusivity 100% and the detection limit of 0.11±0.07 ng of pea DNA corresponding to 12±7 diploid pea genome copies. Using a set of model meat pâtés with defined pea contents, a matrix-related detection limit of 0.05% was determined and a linear calibration line was constructed. The presented analytical method was useful for qualitative detection or semiquantitative determination of pea in food products. The method was relatively fast because the analysis could be performed in one working day.</description><identifier>ISSN: 1438-2377</identifier><identifier>EISSN: 1438-2385</identifier><identifier>DOI: 10.1007/s00217-005-0168-x</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>allergens ; Biological and medical sciences ; chloroplast DNA ; detection ; exons ; Food ; food composition ; Food industries ; foods ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; introns ; Methods of analysis, processing and quality control, regulation, standards ; Microbiology ; molecular sequence data ; Peas ; polymerase chain reaction ; protein concentrates ; quantitative analysis</subject><ispartof>European food research & technology, 2006-03, Vol.222 (5-6), p.600-603</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-55371d7577954b8d3427f92890c98950f335162ac63f2b284c878ec95150b7c13</citedby><cites>FETCH-LOGICAL-c326t-55371d7577954b8d3427f92890c98950f335162ac63f2b284c878ec95150b7c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17614943$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Brežná, B</creatorcontrib><creatorcontrib>Hudecová, L</creatorcontrib><creatorcontrib>Kuchta, T</creatorcontrib><title>Detection of pea in food by real-time polymerase chain reaction (PCR)</title><title>European food research & technology</title><description>A qualitative 5'-nuclease real-time PCR-based method for the detection of pea (Pisum sativum) in food is described. The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent PCR with pea-specific primers and a TaqMan fluorescent probe. The primers and the probe are oriented to the chloroplast DNA intron located between trnL and trnF exons encoding for tRNA. The analytical parameters of the method were inclusivity 100%, exclusivity 100% and the detection limit of 0.11±0.07 ng of pea DNA corresponding to 12±7 diploid pea genome copies. Using a set of model meat pâtés with defined pea contents, a matrix-related detection limit of 0.05% was determined and a linear calibration line was constructed. The presented analytical method was useful for qualitative detection or semiquantitative determination of pea in food products. The method was relatively fast because the analysis could be performed in one working day.</description><subject>allergens</subject><subject>Biological and medical sciences</subject><subject>chloroplast DNA</subject><subject>detection</subject><subject>exons</subject><subject>Food</subject><subject>food composition</subject><subject>Food industries</subject><subject>foods</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>introns</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Microbiology</subject><subject>molecular sequence data</subject><subject>Peas</subject><subject>polymerase chain reaction</subject><subject>protein concentrates</subject><subject>quantitative analysis</subject><issn>1438-2377</issn><issn>1438-2385</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpFkE1LAzEURYMoWKs_wJVBEHQxms9JspRaP6CgqF2HTJrolOlkTKbQ_ntTpujqPXjn3gcHgHOMbjFC4i4hRLAoEOIFwqUsNgdghBmVBaGSH_7tQhyDk5SWmVMlZiMwfXC9s30dWhg87JyBdQt9CAtYbWF0pin6euVgF5rtykWTHLTfJiP5NKSu3ybvN6fgyJsmubP9HIP54_Rz8lzMXp9eJvezwlJS9gXnVOCF4EIoziq5oIwIr4hUyCqpOPKUclwSY0vqSUUks1JIZxXHHFXCYjoGl0NvF8PP2qVeL8M6tvmlLinBXBJOMoQHyMaQUnRed7FembjVGOmdLD3I0lmC3snSm5y52hebZE3jo2ltnf6DIstSjGbuYuC8Cdp8xczMPwjCFGGkMGWM_gKqhG-l</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Brežná, B</creator><creator>Hudecová, L</creator><creator>Kuchta, T</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QR</scope><scope>7RQ</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X2</scope><scope>7XB</scope><scope>87Z</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>F~G</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>L.-</scope><scope>L6V</scope><scope>M0C</scope><scope>M0K</scope><scope>M2P</scope><scope>M7S</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20060301</creationdate><title>Detection of pea in food by real-time polymerase chain reaction (PCR)</title><author>Brežná, B ; Hudecová, L ; Kuchta, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-55371d7577954b8d3427f92890c98950f335162ac63f2b284c878ec95150b7c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>allergens</topic><topic>Biological and medical sciences</topic><topic>chloroplast DNA</topic><topic>detection</topic><topic>exons</topic><topic>Food</topic><topic>food composition</topic><topic>Food industries</topic><topic>foods</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>introns</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>Microbiology</topic><topic>molecular sequence data</topic><topic>Peas</topic><topic>polymerase chain reaction</topic><topic>protein concentrates</topic><topic>quantitative analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brežná, B</creatorcontrib><creatorcontrib>Hudecová, L</creatorcontrib><creatorcontrib>Kuchta, T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Career & Technical Education Database</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Access via ABI/INFORM (ProQuest)</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Engineering Collection</collection><collection>ABI/INFORM Global</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><jtitle>European food research & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brežná, B</au><au>Hudecová, L</au><au>Kuchta, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of pea in food by real-time polymerase chain reaction (PCR)</atitle><jtitle>European food research & technology</jtitle><date>2006-03-01</date><risdate>2006</risdate><volume>222</volume><issue>5-6</issue><spage>600</spage><epage>603</epage><pages>600-603</pages><issn>1438-2377</issn><eissn>1438-2385</eissn><abstract>A qualitative 5'-nuclease real-time PCR-based method for the detection of pea (Pisum sativum) in food is described. The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent PCR with pea-specific primers and a TaqMan fluorescent probe. The primers and the probe are oriented to the chloroplast DNA intron located between trnL and trnF exons encoding for tRNA. The analytical parameters of the method were inclusivity 100%, exclusivity 100% and the detection limit of 0.11±0.07 ng of pea DNA corresponding to 12±7 diploid pea genome copies. Using a set of model meat pâtés with defined pea contents, a matrix-related detection limit of 0.05% was determined and a linear calibration line was constructed. The presented analytical method was useful for qualitative detection or semiquantitative determination of pea in food products. The method was relatively fast because the analysis could be performed in one working day.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s00217-005-0168-x</doi><tpages>4</tpages></addata></record> |
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| subjects | allergens Biological and medical sciences chloroplast DNA detection exons Food food composition Food industries foods Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General aspects introns Methods of analysis, processing and quality control, regulation, standards Microbiology molecular sequence data Peas polymerase chain reaction protein concentrates quantitative analysis |
| title | Detection of pea in food by real-time polymerase chain reaction (PCR) |
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