In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2
Chromium-resistant bacteria (CRB) isolated from soils can be used to reduce toxic Cr(VI) from contaminated environments. This study assessed in vitro reduction of hexavalent Cr using a cell-free extract (CFE) of CRB isolated from soil contaminated with dichromate. One isolate, ES 29, that substantia...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2003-10, Vol.62 (5-6), p.569-573 |
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| creator | CAMARGO, F. A. O OKEKE, B. C BENTO, F. M FRANKENBERGER, W. T |
| description | Chromium-resistant bacteria (CRB) isolated from soils can be used to reduce toxic Cr(VI) from contaminated environments. This study assessed in vitro reduction of hexavalent Cr using a cell-free extract (CFE) of CRB isolated from soil contaminated with dichromate. One isolate, ES 29, that substantially reduced Cr(VI) was identified as a Bacillus species by 16S rRNA gene-sequence homology. The isolate reduced Cr(VI) under aerobic conditions, using NADH as an electron donor and produced a soluble Cr(VI)-reducing enzyme stimulated by copper (Cu2+). The CFE of the bacterial isolate reduced 50% of Cr(VI) in 6 h. The Cr(VI)-reduction activity of the CFE had a Km of 7.09 microM and a Vmax of 0.171 micromol min(-1) mg(-1) protein. Mercury inhibited the enzyme, but not competitively, with a Vmax of 0.143 micromol min(-1) mg(-1) protein, a Km of 7.07 microM and a Ki of 1.58 microM. This study characterizes the enzymatic reduction of Cr(VI) by Bacillus sp. ES 29 which can be used for the bioremediation of chromate. |
| doi_str_mv | 10.1007/s00253-003-1291-x |
| format | Article |
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ES 29 stimulated by Cu2</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>CAMARGO, F. A. O ; OKEKE, B. C ; BENTO, F. M ; FRANKENBERGER, W. T</creator><creatorcontrib>CAMARGO, F. A. O ; OKEKE, B. C ; BENTO, F. M ; FRANKENBERGER, W. T</creatorcontrib><description>Chromium-resistant bacteria (CRB) isolated from soils can be used to reduce toxic Cr(VI) from contaminated environments. This study assessed in vitro reduction of hexavalent Cr using a cell-free extract (CFE) of CRB isolated from soil contaminated with dichromate. One isolate, ES 29, that substantially reduced Cr(VI) was identified as a Bacillus species by 16S rRNA gene-sequence homology. The isolate reduced Cr(VI) under aerobic conditions, using NADH as an electron donor and produced a soluble Cr(VI)-reducing enzyme stimulated by copper (Cu2+). The CFE of the bacterial isolate reduced 50% of Cr(VI) in 6 h. The Cr(VI)-reduction activity of the CFE had a Km of 7.09 microM and a Vmax of 0.171 micromol min(-1) mg(-1) protein. Mercury inhibited the enzyme, but not competitively, with a Vmax of 0.143 micromol min(-1) mg(-1) protein, a Km of 7.07 microM and a Ki of 1.58 microM. This study characterizes the enzymatic reduction of Cr(VI) by Bacillus sp. ES 29 which can be used for the bioremediation of chromate.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-003-1291-x</identifier><identifier>PMID: 12679851</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Aerobic conditions ; Bacillus - classification ; Bacillus - drug effects ; Bacillus - enzymology ; Bacillus - isolation & purification ; Bacteria ; Biodegradation, Environmental ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Bioremediation ; Biotechnology ; Cell Fractionation ; Chromium ; Chromium - metabolism ; Chromium - toxicity ; Copper - metabolism ; Drug Resistance, Bacterial ; Enzyme Inhibitors - pharmacology ; Enzymes ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Kinetics ; Mercury ; Mercury - pharmacology ; Microbiology ; Miscellaneous ; Mission oriented research ; NAD - metabolism ; Oxidation-Reduction - drug effects ; Oxidoreductases - antagonists & inhibitors ; Oxidoreductases - metabolism ; Potassium Dichromate ; Soil contamination ; Soil Microbiology ; Soil Pollutants - metabolism</subject><ispartof>Applied microbiology and biotechnology, 2003-10, Vol.62 (5-6), p.569-573</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15248576$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12679851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CAMARGO, F. A. O</creatorcontrib><creatorcontrib>OKEKE, B. C</creatorcontrib><creatorcontrib>BENTO, F. M</creatorcontrib><creatorcontrib>FRANKENBERGER, W. T</creatorcontrib><title>In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>Chromium-resistant bacteria (CRB) isolated from soils can be used to reduce toxic Cr(VI) from contaminated environments. This study assessed in vitro reduction of hexavalent Cr using a cell-free extract (CFE) of CRB isolated from soil contaminated with dichromate. One isolate, ES 29, that substantially reduced Cr(VI) was identified as a Bacillus species by 16S rRNA gene-sequence homology. The isolate reduced Cr(VI) under aerobic conditions, using NADH as an electron donor and produced a soluble Cr(VI)-reducing enzyme stimulated by copper (Cu2+). The CFE of the bacterial isolate reduced 50% of Cr(VI) in 6 h. The Cr(VI)-reduction activity of the CFE had a Km of 7.09 microM and a Vmax of 0.171 micromol min(-1) mg(-1) protein. Mercury inhibited the enzyme, but not competitively, with a Vmax of 0.143 micromol min(-1) mg(-1) protein, a Km of 7.07 microM and a Ki of 1.58 microM. This study characterizes the enzymatic reduction of Cr(VI) by Bacillus sp. ES 29 which can be used for the bioremediation of chromate.</description><subject>Aerobic conditions</subject><subject>Bacillus - classification</subject><subject>Bacillus - drug effects</subject><subject>Bacillus - enzymology</subject><subject>Bacillus - isolation & purification</subject><subject>Bacteria</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Cell Fractionation</subject><subject>Chromium</subject><subject>Chromium - metabolism</subject><subject>Chromium - toxicity</subject><subject>Copper - metabolism</subject><subject>Drug Resistance, Bacterial</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. 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A. O</au><au>OKEKE, B. C</au><au>BENTO, F. M</au><au>FRANKENBERGER, W. T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>62</volume><issue>5-6</issue><spage>569</spage><epage>573</epage><pages>569-573</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Chromium-resistant bacteria (CRB) isolated from soils can be used to reduce toxic Cr(VI) from contaminated environments. This study assessed in vitro reduction of hexavalent Cr using a cell-free extract (CFE) of CRB isolated from soil contaminated with dichromate. One isolate, ES 29, that substantially reduced Cr(VI) was identified as a Bacillus species by 16S rRNA gene-sequence homology. The isolate reduced Cr(VI) under aerobic conditions, using NADH as an electron donor and produced a soluble Cr(VI)-reducing enzyme stimulated by copper (Cu2+). The CFE of the bacterial isolate reduced 50% of Cr(VI) in 6 h. The Cr(VI)-reduction activity of the CFE had a Km of 7.09 microM and a Vmax of 0.171 micromol min(-1) mg(-1) protein. Mercury inhibited the enzyme, but not competitively, with a Vmax of 0.143 micromol min(-1) mg(-1) protein, a Km of 7.07 microM and a Ki of 1.58 microM. This study characterizes the enzymatic reduction of Cr(VI) by Bacillus sp. ES 29 which can be used for the bioremediation of chromate.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12679851</pmid><doi>10.1007/s00253-003-1291-x</doi><tpages>5</tpages></addata></record> |
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| subjects | Aerobic conditions Bacillus - classification Bacillus - drug effects Bacillus - enzymology Bacillus - isolation & purification Bacteria Biodegradation, Environmental Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Bioremediation Biotechnology Cell Fractionation Chromium Chromium - metabolism Chromium - toxicity Copper - metabolism Drug Resistance, Bacterial Enzyme Inhibitors - pharmacology Enzymes Fundamental and applied biological sciences. Psychology In Vitro Techniques Kinetics Mercury Mercury - pharmacology Microbiology Miscellaneous Mission oriented research NAD - metabolism Oxidation-Reduction - drug effects Oxidoreductases - antagonists & inhibitors Oxidoreductases - metabolism Potassium Dichromate Soil contamination Soil Microbiology Soil Pollutants - metabolism |
| title | In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2 |
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