Immobilization as a technical possibility for long-term storage of bacterial biosensors
For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE ope...
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| Veröffentlicht in: | Radiation and environmental biophysics 2005-05, Vol.44 (1), p.69-71 |
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| container_title | Radiation and environmental biophysics |
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| creator | Park, Kyeong Seo Baumstark-Khan, Christa Rettberg, Petra Horneck, Gerda Rabbow, Elke Gu, Man Bock |
| description | For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 10(10) CFU/ml in microtiter plates and stored up to 6 weeks at 4 degrees C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples. |
| doi_str_mv | 10.1007/s00411-005-0271-1 |
| format | Article |
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Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 10(10) CFU/ml in microtiter plates and stored up to 6 weeks at 4 degrees C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.</description><identifier>ISSN: 0301-634X</identifier><identifier>EISSN: 1432-2099</identifier><identifier>DOI: 10.1007/s00411-005-0271-1</identifier><identifier>PMID: 15791471</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Bacteria ; Bioluminescence ; Biosensing Techniques ; Biosensors ; DNA Damage ; Environmental Monitoring - methods ; Field tests ; Luminescent Measurements ; Mitomycin - toxicity ; Mutagenicity Tests - methods ; Mutagens - toxicity ; Salmonella typhimurium - drug effects ; Salmonella typhimurium - genetics ; SOS Response (Genetics) - genetics ; Time Factors</subject><ispartof>Radiation and environmental biophysics, 2005-05, Vol.44 (1), p.69-71</ispartof><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-dab49ba12fbc6bdd03dff0ee8120a08b86f7df24103fbe043cc48255027155e93</citedby><cites>FETCH-LOGICAL-c326t-dab49ba12fbc6bdd03dff0ee8120a08b86f7df24103fbe043cc48255027155e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15791471$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Kyeong Seo</creatorcontrib><creatorcontrib>Baumstark-Khan, Christa</creatorcontrib><creatorcontrib>Rettberg, Petra</creatorcontrib><creatorcontrib>Horneck, Gerda</creatorcontrib><creatorcontrib>Rabbow, Elke</creatorcontrib><creatorcontrib>Gu, Man Bock</creatorcontrib><title>Immobilization as a technical possibility for long-term storage of bacterial biosensors</title><title>Radiation and environmental biophysics</title><addtitle>Radiat Environ Biophys</addtitle><description>For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 10(10) CFU/ml in microtiter plates and stored up to 6 weeks at 4 degrees C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.</description><subject>Bacteria</subject><subject>Bioluminescence</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>DNA Damage</subject><subject>Environmental Monitoring - methods</subject><subject>Field tests</subject><subject>Luminescent Measurements</subject><subject>Mitomycin - toxicity</subject><subject>Mutagenicity Tests - methods</subject><subject>Mutagens - toxicity</subject><subject>Salmonella typhimurium - drug effects</subject><subject>Salmonella typhimurium - genetics</subject><subject>SOS Response (Genetics) - genetics</subject><subject>Time Factors</subject><issn>0301-634X</issn><issn>1432-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpFkM1LAzEQxYMotlb_AC8SvEdnkv08SvGjUPCi6C0k2aSm7G5qsj3oX-8uLXgamHnvDe9HyDXCHQKU9wkgQ2QAOQNeIsMTMsdMcMahrk_JHAQgK0T2OSMXKW0BsCyK-pzMMC9rzEqck49V1wXtW_-rBh96qhJVdLDmq_dGtXQXUvLTefihLkTahn7DBhs7moYQ1cbS4KhWZlz5Ua59SLZPIaZLcuZUm-zVcS7I-9Pj2_KFrV-fV8uHNTOCFwNrlM5qrZA7bQrdNCAa58DaCjkoqHRVuLJxPEMQTlvIhDFZxfN8qpvnthYLcnvI3cXwvbdpkNuwj_34UhYo8qriHEcRHkQmjn2idXIXfafij0SQE0l5IClHknKKlpPn5hi8151t_h1HdOIPGVtvoA</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Park, Kyeong Seo</creator><creator>Baumstark-Khan, Christa</creator><creator>Rettberg, Petra</creator><creator>Horneck, Gerda</creator><creator>Rabbow, Elke</creator><creator>Gu, Man Bock</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>SOI</scope></search><sort><creationdate>200505</creationdate><title>Immobilization as a technical possibility for long-term storage of bacterial biosensors</title><author>Park, Kyeong Seo ; 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Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 10(10) CFU/ml in microtiter plates and stored up to 6 weeks at 4 degrees C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>15791471</pmid><doi>10.1007/s00411-005-0271-1</doi><tpages>3</tpages></addata></record> |
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| subjects | Bacteria Bioluminescence Biosensing Techniques Biosensors DNA Damage Environmental Monitoring - methods Field tests Luminescent Measurements Mitomycin - toxicity Mutagenicity Tests - methods Mutagens - toxicity Salmonella typhimurium - drug effects Salmonella typhimurium - genetics SOS Response (Genetics) - genetics Time Factors |
| title | Immobilization as a technical possibility for long-term storage of bacterial biosensors |
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