Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH
Laser microdissection was used for the preparation of whole chromosome painting probes in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 mill...
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| Veröffentlicht in: | Chromosome research 2010-06, Vol.18 (4), p.431-439 |
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| creator | Krylov, Vladimir Kubickova, Svatava Rubes, Jiri Macha, Jaroslav Tlapakova, Tereza Seifertova, Eva Sebkova, Natasa |
| description | Laser microdissection was used for the preparation of whole chromosome painting probes in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 million years of separate evolution. Their arrangement is in a partial concordance with previous experiments based on similarity of a high-resolution replication banding pattern. Further support for an allotetraploid origin of X. laevis was given by hybridization with a probe derived from the smallest X. tropicalis chromosome (Xt10). Here, pericentric areas of both arms of Xl 14 and 18 were stained, indicating intrachromosomal rearrangements. The positions of signals were not in agreement with the chromosomal quartets revealed by painting probes Xt 8 and 9 (Xl 11 + 14 and Xl 15 + 18, respectively). This suggests that both X. tropicalis chromosomes underwent non-reciprocal translocation of Xt10 separately in at least two different ancient ancestors. In addition, the observed translocation events could explain the origin of individuals with 18 chromosomes in diploid karyotypes, probably extinct after the genesis of the allotetraploid X. laevis (2n = 36). |
| doi_str_mv | 10.1007/s10577-010-9127-x |
| format | Article |
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Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 million years of separate evolution. Their arrangement is in a partial concordance with previous experiments based on similarity of a high-resolution replication banding pattern. Further support for an allotetraploid origin of X. laevis was given by hybridization with a probe derived from the smallest X. tropicalis chromosome (Xt10). Here, pericentric areas of both arms of Xl 14 and 18 were stained, indicating intrachromosomal rearrangements. The positions of signals were not in agreement with the chromosomal quartets revealed by painting probes Xt 8 and 9 (Xl 11 + 14 and Xl 15 + 18, respectively). This suggests that both X. tropicalis chromosomes underwent non-reciprocal translocation of Xt10 separately in at least two different ancient ancestors. In addition, the observed translocation events could explain the origin of individuals with 18 chromosomes in diploid karyotypes, probably extinct after the genesis of the allotetraploid X. laevis (2n = 36).</description><identifier>ISSN: 0967-3849</identifier><identifier>EISSN: 1573-6849</identifier><identifier>DOI: 10.1007/s10577-010-9127-x</identifier><identifier>PMID: 20390340</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Animal Genetics and Genomics ; Animals ; Biodiversity ; Biological Evolution ; Biomedical and Life Sciences ; Cell Biology ; Cellular biology ; Chromosome Banding ; Chromosomes ; Chromosomes - genetics ; Cytogenetic Analysis - methods ; DNA Probes ; Fluorescence in situ hybridization ; Frogs ; Genomics ; Human Genetics ; In Situ Hybridization, Fluorescence ; Karyotyping ; Life Sciences ; Microdissection ; Plant Genetics and Genomics ; Translocation, Genetic ; whole chromosome painting probe ; Xenopus - genetics ; Xenopus laevis ; Xenopus laevis - genetics ; Xenopus tropicalis ; Zoo-FISH</subject><ispartof>Chromosome research, 2010-06, Vol.18 (4), p.431-439</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-5ea73beada5dfd79dbceae502a581d0bfd4b16bd88bafc4c13f459d917ecb77a3</citedby><cites>FETCH-LOGICAL-c460t-5ea73beada5dfd79dbceae502a581d0bfd4b16bd88bafc4c13f459d917ecb77a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10577-010-9127-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10577-010-9127-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20390340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krylov, Vladimir</creatorcontrib><creatorcontrib>Kubickova, Svatava</creatorcontrib><creatorcontrib>Rubes, Jiri</creatorcontrib><creatorcontrib>Macha, Jaroslav</creatorcontrib><creatorcontrib>Tlapakova, Tereza</creatorcontrib><creatorcontrib>Seifertova, Eva</creatorcontrib><creatorcontrib>Sebkova, Natasa</creatorcontrib><title>Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH</title><title>Chromosome research</title><addtitle>Chromosome Res</addtitle><addtitle>Chromosome Res</addtitle><description>Laser microdissection was used for the preparation of whole chromosome painting probes in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 million years of separate evolution. Their arrangement is in a partial concordance with previous experiments based on similarity of a high-resolution replication banding pattern. Further support for an allotetraploid origin of X. laevis was given by hybridization with a probe derived from the smallest X. tropicalis chromosome (Xt10). Here, pericentric areas of both arms of Xl 14 and 18 were stained, indicating intrachromosomal rearrangements. The positions of signals were not in agreement with the chromosomal quartets revealed by painting probes Xt 8 and 9 (Xl 11 + 14 and Xl 15 + 18, respectively). This suggests that both X. tropicalis chromosomes underwent non-reciprocal translocation of Xt10 separately in at least two different ancient ancestors. In addition, the observed translocation events could explain the origin of individuals with 18 chromosomes in diploid karyotypes, probably extinct after the genesis of the allotetraploid X. laevis (2n = 36).</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biodiversity</subject><subject>Biological Evolution</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cellular biology</subject><subject>Chromosome Banding</subject><subject>Chromosomes</subject><subject>Chromosomes - genetics</subject><subject>Cytogenetic Analysis - methods</subject><subject>DNA Probes</subject><subject>Fluorescence in situ hybridization</subject><subject>Frogs</subject><subject>Genomics</subject><subject>Human Genetics</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Karyotyping</subject><subject>Life Sciences</subject><subject>Microdissection</subject><subject>Plant Genetics and Genomics</subject><subject>Translocation, Genetic</subject><subject>whole chromosome painting probe</subject><subject>Xenopus - genetics</subject><subject>Xenopus laevis</subject><subject>Xenopus laevis - genetics</subject><subject>Xenopus tropicalis</subject><subject>Zoo-FISH</subject><issn>0967-3849</issn><issn>1573-6849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1DAUhS0EokPhAdiAxd7lOo7HyRJVlFaqBFKphNhY_rmZuiRxsBPoPA2vioeUsmNlWz7nO_fqEPKSwwkHUG8zB6kUAw6s5ZVid4_Ihksl2Lap28dkA-1WMVHuR-RZzrcA0IiaPyVHFYgWRA0b8utTwskkM4c40tjRLzjGacl0TnEKzvQh0583sUfqblIcYo4D0smEcQ7jjk4pWsx0yYdHbzImOgSXog85o_uDNKOnCV0c85wW95ByUuT4o8BnnJOZ-hg8_WbSPs77Cand068xsrOLq_Pn5Eln-owv7s9jcn32_vPpObv8-OHi9N0lc_UWZibRKGHReCN951XrrUODEiojG-7Bdr62fGt901jTudpx0dWy9S1X6KxSRhyTNyu37PR9wTzr27iksURqCYJL0cqqiPgqKjvmnLDTUwpDGVtz0IdG9NqILo3oQyP6rnhe3YMXO6B_cPytoAiqVZDL17jD9C_5f9TXq6kzUZtdCllfX1XABfBGqqrm4jealKYb</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Krylov, Vladimir</creator><creator>Kubickova, Svatava</creator><creator>Rubes, Jiri</creator><creator>Macha, Jaroslav</creator><creator>Tlapakova, Tereza</creator><creator>Seifertova, Eva</creator><creator>Sebkova, Natasa</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20100601</creationdate><title>Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH</title><author>Krylov, Vladimir ; Kubickova, Svatava ; Rubes, Jiri ; Macha, Jaroslav ; Tlapakova, Tereza ; Seifertova, Eva ; Sebkova, Natasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-5ea73beada5dfd79dbceae502a581d0bfd4b16bd88bafc4c13f459d917ecb77a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biodiversity</topic><topic>Biological Evolution</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cellular biology</topic><topic>Chromosome Banding</topic><topic>Chromosomes</topic><topic>Chromosomes - genetics</topic><topic>Cytogenetic Analysis - methods</topic><topic>DNA Probes</topic><topic>Fluorescence in situ hybridization</topic><topic>Frogs</topic><topic>Genomics</topic><topic>Human Genetics</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Karyotyping</topic><topic>Life Sciences</topic><topic>Microdissection</topic><topic>Plant Genetics and Genomics</topic><topic>Translocation, Genetic</topic><topic>whole chromosome painting probe</topic><topic>Xenopus - genetics</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis - genetics</topic><topic>Xenopus tropicalis</topic><topic>Zoo-FISH</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krylov, Vladimir</creatorcontrib><creatorcontrib>Kubickova, Svatava</creatorcontrib><creatorcontrib>Rubes, Jiri</creatorcontrib><creatorcontrib>Macha, Jaroslav</creatorcontrib><creatorcontrib>Tlapakova, Tereza</creatorcontrib><creatorcontrib>Seifertova, Eva</creatorcontrib><creatorcontrib>Sebkova, Natasa</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Chromosome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krylov, Vladimir</au><au>Kubickova, Svatava</au><au>Rubes, Jiri</au><au>Macha, Jaroslav</au><au>Tlapakova, Tereza</au><au>Seifertova, Eva</au><au>Sebkova, Natasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH</atitle><jtitle>Chromosome research</jtitle><stitle>Chromosome Res</stitle><addtitle>Chromosome Res</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>18</volume><issue>4</issue><spage>431</spage><epage>439</epage><pages>431-439</pages><issn>0967-3849</issn><eissn>1573-6849</eissn><abstract>Laser microdissection was used for the preparation of whole chromosome painting probes in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 million years of separate evolution. Their arrangement is in a partial concordance with previous experiments based on similarity of a high-resolution replication banding pattern. Further support for an allotetraploid origin of X. laevis was given by hybridization with a probe derived from the smallest X. tropicalis chromosome (Xt10). Here, pericentric areas of both arms of Xl 14 and 18 were stained, indicating intrachromosomal rearrangements. The positions of signals were not in agreement with the chromosomal quartets revealed by painting probes Xt 8 and 9 (Xl 11 + 14 and Xl 15 + 18, respectively). This suggests that both X. tropicalis chromosomes underwent non-reciprocal translocation of Xt10 separately in at least two different ancient ancestors. In addition, the observed translocation events could explain the origin of individuals with 18 chromosomes in diploid karyotypes, probably extinct after the genesis of the allotetraploid X. laevis (2n = 36).</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>20390340</pmid><doi>10.1007/s10577-010-9127-x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Chromosome research, 2010-06, Vol.18 (4), p.431-439 |
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| subjects | Animal Genetics and Genomics Animals Biodiversity Biological Evolution Biomedical and Life Sciences Cell Biology Cellular biology Chromosome Banding Chromosomes Chromosomes - genetics Cytogenetic Analysis - methods DNA Probes Fluorescence in situ hybridization Frogs Genomics Human Genetics In Situ Hybridization, Fluorescence Karyotyping Life Sciences Microdissection Plant Genetics and Genomics Translocation, Genetic whole chromosome painting probe Xenopus - genetics Xenopus laevis Xenopus laevis - genetics Xenopus tropicalis Zoo-FISH |
| title | Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH |
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