Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania
Our study investigated the type of acquired metallo-β-lactamases (MBLs) produced by carbapenem-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from five hospitals in Iasi, Romania and the genetic relatedness of the strains carrying MBL genes. Of 106 carbapenem-resis...
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| Veröffentlicht in: | Revista română de medicină de laborator 2013, Vol.21 (4), p.423-430 |
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| description | Our study investigated the type of acquired metallo-β-lactamases (MBLs) produced by carbapenem-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from five hospitals in Iasi, Romania and the genetic relatedness of the strains carrying MBL genes. Of 106 carbapenem-resistant strains, 50 were positive for MBL production after screening with Etest MBL. PCR analysis showed that 46 isolates (44 P. aeruginosa and 2 A. baumannii) carried a blaVIM gene. By DNA sequencing we identified two class 1 integrons carrying the blaVIM gene in the P. aeruginosa: IntI1-aacA7-blaVIM-2-qacEΔ1 in 43 strains, and IntI1-aacA7- ΔblaVIM-ΔcmlA1-qacEΔ1 in one strain. In A. baumannii isolates the blaVIM-2 gene was not associated with a class 1 integron. Random amplified polymorphic DNA typing of VIM-2-producing P. aeruginosa strains revealed the presence of a major RAPD type in all five hospitals. Early detection and surveillance of such strains must be accompanied by rigorous infection control measures in order to limit the spread of MBLs in our clinical settings
Studiul nostru a investigat tipul de metalo-β-lactamaze (MBL) produse de Pseudomonas aeruginosa şi Acinetobacter baumannii rezistente la carbapeneme, izolate în cinci spitale din Iaşi, Romania şi inrudirea genetică a acestor tulpini. Din 106 tulpini rezistente la carbapeneme, 50 au dat rezultate pozitive la screening-ul prin Etest pentru producerea de MBL. Analiza PCR a arătat ca 46 dintre izolate (44 P. aeruginosa si 2 A. baumannii) aveau gena blaVIM. La tulpinile de P. aeruginosa, secvenţierea a demonstrat prezenţa a doi integroni de clasa 1 care conţin gena blaVIM: IntI1-aacA7-blaVIM-2-qacEΔ1, la 43 tulpini, respectiv IntI1-aacA7-ΔblaVIM-ΔcmlA1- qacEΔ1, la o singură tulpină. La tulpinile de A. baumannii gena blaVIM-2 nu a fost detectată într-un integron de clasa 1. Tiparea prin RAPD (Random amplified polymorphic DNA typing) a tulpinilor de P. aeruginosa producătoare de VIM-2 a evidenţiat prezenţa unui tip RAPD major, în toate cele cinci spitale. Detectarea precoce şi supravegherea unor astfel de izolate trebuie insoţită de măsuri riguroase de control al infecţiei, în vederea limitării răspândirii MBL în unitatile spitalicesti |
| doi_str_mv | 10.2478/rrlm-2013-0035 |
| format | Article |
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Studiul nostru a investigat tipul de metalo-β-lactamaze (MBL) produse de Pseudomonas aeruginosa şi Acinetobacter baumannii rezistente la carbapeneme, izolate în cinci spitale din Iaşi, Romania şi inrudirea genetică a acestor tulpini. Din 106 tulpini rezistente la carbapeneme, 50 au dat rezultate pozitive la screening-ul prin Etest pentru producerea de MBL. Analiza PCR a arătat ca 46 dintre izolate (44 P. aeruginosa si 2 A. baumannii) aveau gena blaVIM. La tulpinile de P. aeruginosa, secvenţierea a demonstrat prezenţa a doi integroni de clasa 1 care conţin gena blaVIM: IntI1-aacA7-blaVIM-2-qacEΔ1, la 43 tulpini, respectiv IntI1-aacA7-ΔblaVIM-ΔcmlA1- qacEΔ1, la o singură tulpină. La tulpinile de A. baumannii gena blaVIM-2 nu a fost detectată într-un integron de clasa 1. Tiparea prin RAPD (Random amplified polymorphic DNA typing) a tulpinilor de P. aeruginosa producătoare de VIM-2 a evidenţiat prezenţa unui tip RAPD major, în toate cele cinci spitale. Detectarea precoce şi supravegherea unor astfel de izolate trebuie insoţită de măsuri riguroase de control al infecţiei, în vederea limitării răspândirii MBL în unitatile spitalicesti</description><identifier>ISSN: 2284-5623</identifier><identifier>ISSN: 1841-6624</identifier><identifier>EISSN: 2284-5623</identifier><identifier>DOI: 10.2478/rrlm-2013-0035</identifier><language>eng</language><publisher>Târgu Mureș: Versita</publisher><subject>Acinetobacter baumannii ; metallo-beta-lactamase ; metalo-beta-lactamaze ; Pseudomonas aeruginosa ; VIM-2</subject><ispartof>Revista română de medicină de laborator, 2013, Vol.21 (4), p.423-430</ispartof><rights>20130101.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-5a7a5f2f5862fbab33eebf6b1e7c52dd7d5c09e410012cd0542178a63d7afb343</citedby><cites>FETCH-LOGICAL-c360t-5a7a5f2f5862fbab33eebf6b1e7c52dd7d5c09e410012cd0542178a63d7afb343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Mereuţă, Ana Irina</creatorcontrib><creatorcontrib>Bădescu, Aida Corina</creatorcontrib><creatorcontrib>Dorneanu, Olivia Simona</creatorcontrib><creatorcontrib>Iancu, Luminiţa Smaranda</creatorcontrib><creatorcontrib>Tuchiluş, Cristina Gabriela</creatorcontrib><title>Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania</title><title>Revista română de medicină de laborator</title><description>Our study investigated the type of acquired metallo-β-lactamases (MBLs) produced by carbapenem-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from five hospitals in Iasi, Romania and the genetic relatedness of the strains carrying MBL genes. Of 106 carbapenem-resistant strains, 50 were positive for MBL production after screening with Etest MBL. PCR analysis showed that 46 isolates (44 P. aeruginosa and 2 A. baumannii) carried a blaVIM gene. By DNA sequencing we identified two class 1 integrons carrying the blaVIM gene in the P. aeruginosa: IntI1-aacA7-blaVIM-2-qacEΔ1 in 43 strains, and IntI1-aacA7- ΔblaVIM-ΔcmlA1-qacEΔ1 in one strain. In A. baumannii isolates the blaVIM-2 gene was not associated with a class 1 integron. Random amplified polymorphic DNA typing of VIM-2-producing P. aeruginosa strains revealed the presence of a major RAPD type in all five hospitals. Early detection and surveillance of such strains must be accompanied by rigorous infection control measures in order to limit the spread of MBLs in our clinical settings
Studiul nostru a investigat tipul de metalo-β-lactamaze (MBL) produse de Pseudomonas aeruginosa şi Acinetobacter baumannii rezistente la carbapeneme, izolate în cinci spitale din Iaşi, Romania şi inrudirea genetică a acestor tulpini. Din 106 tulpini rezistente la carbapeneme, 50 au dat rezultate pozitive la screening-ul prin Etest pentru producerea de MBL. Analiza PCR a arătat ca 46 dintre izolate (44 P. aeruginosa si 2 A. baumannii) aveau gena blaVIM. La tulpinile de P. aeruginosa, secvenţierea a demonstrat prezenţa a doi integroni de clasa 1 care conţin gena blaVIM: IntI1-aacA7-blaVIM-2-qacEΔ1, la 43 tulpini, respectiv IntI1-aacA7-ΔblaVIM-ΔcmlA1- qacEΔ1, la o singură tulpină. La tulpinile de A. baumannii gena blaVIM-2 nu a fost detectată într-un integron de clasa 1. Tiparea prin RAPD (Random amplified polymorphic DNA typing) a tulpinilor de P. aeruginosa producătoare de VIM-2 a evidenţiat prezenţa unui tip RAPD major, în toate cele cinci spitale. Detectarea precoce şi supravegherea unor astfel de izolate trebuie insoţită de măsuri riguroase de control al infecţiei, în vederea limitării răspândirii MBL în unitatile spitalicesti</description><subject>Acinetobacter baumannii</subject><subject>metallo-beta-lactamase</subject><subject>metalo-beta-lactamaze</subject><subject>Pseudomonas aeruginosa</subject><subject>VIM-2</subject><issn>2284-5623</issn><issn>1841-6624</issn><issn>2284-5623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkMtKBDEQRRtRUNSt64Bbo3l294AbER8DiuJr21TnIZF0MibdiFt_xK_xv8wwgi6sza3FvbeoU1V7lBwy0bRHKfkBM0I5JoTLtWqLsVZgWTO-_mffrHZzfiFlZE3bRmxVH_eLZECjaNHT_BozNJgRvI-4L4o9qBEGyAa5gG6zmXQcYoCMwKTp2YWYAUHQ6ES5YMbYF7tJqIdpgBCcQ8q74BR45HL0MJqMbIoDmsPXpztAd7HYHOxUGxZ8Nrs_ul09np89nF7iq5uL-enJFVa8JiOW0IC0zMq2ZraHnnNjelv31DRKMq0bLRWZGUEJoUxpIgWjTQs11w3Yngu-Xe2vehcpvk4mj91LnFIoJztOZTMTVMxmxXW4cqkUc07GdovkBkjvHSXdEnW3RN0tUXdL1CVwvAq8gS_fa_Ocpvey_Lb_H2RUCMb5NzA1iA0</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>Mereuţă, Ana Irina</creator><creator>Bădescu, Aida Corina</creator><creator>Dorneanu, Olivia Simona</creator><creator>Iancu, Luminiţa Smaranda</creator><creator>Tuchiluş, Cristina Gabriela</creator><general>Versita</general><general>De Gruyter Poland</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>2013</creationdate><title>Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania</title><author>Mereuţă, Ana Irina ; Bădescu, Aida Corina ; Dorneanu, Olivia Simona ; Iancu, Luminiţa Smaranda ; Tuchiluş, Cristina Gabriela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-5a7a5f2f5862fbab33eebf6b1e7c52dd7d5c09e410012cd0542178a63d7afb343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acinetobacter baumannii</topic><topic>metallo-beta-lactamase</topic><topic>metalo-beta-lactamaze</topic><topic>Pseudomonas aeruginosa</topic><topic>VIM-2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mereuţă, Ana Irina</creatorcontrib><creatorcontrib>Bădescu, Aida Corina</creatorcontrib><creatorcontrib>Dorneanu, Olivia Simona</creatorcontrib><creatorcontrib>Iancu, Luminiţa Smaranda</creatorcontrib><creatorcontrib>Tuchiluş, Cristina Gabriela</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Revista română de medicină de laborator</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mereuţă, Ana Irina</au><au>Bădescu, Aida Corina</au><au>Dorneanu, Olivia Simona</au><au>Iancu, Luminiţa Smaranda</au><au>Tuchiluş, Cristina Gabriela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania</atitle><jtitle>Revista română de medicină de laborator</jtitle><date>2013</date><risdate>2013</risdate><volume>21</volume><issue>4</issue><spage>423</spage><epage>430</epage><pages>423-430</pages><issn>2284-5623</issn><issn>1841-6624</issn><eissn>2284-5623</eissn><abstract>Our study investigated the type of acquired metallo-β-lactamases (MBLs) produced by carbapenem-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from five hospitals in Iasi, Romania and the genetic relatedness of the strains carrying MBL genes. Of 106 carbapenem-resistant strains, 50 were positive for MBL production after screening with Etest MBL. PCR analysis showed that 46 isolates (44 P. aeruginosa and 2 A. baumannii) carried a blaVIM gene. By DNA sequencing we identified two class 1 integrons carrying the blaVIM gene in the P. aeruginosa: IntI1-aacA7-blaVIM-2-qacEΔ1 in 43 strains, and IntI1-aacA7- ΔblaVIM-ΔcmlA1-qacEΔ1 in one strain. In A. baumannii isolates the blaVIM-2 gene was not associated with a class 1 integron. Random amplified polymorphic DNA typing of VIM-2-producing P. aeruginosa strains revealed the presence of a major RAPD type in all five hospitals. Early detection and surveillance of such strains must be accompanied by rigorous infection control measures in order to limit the spread of MBLs in our clinical settings
Studiul nostru a investigat tipul de metalo-β-lactamaze (MBL) produse de Pseudomonas aeruginosa şi Acinetobacter baumannii rezistente la carbapeneme, izolate în cinci spitale din Iaşi, Romania şi inrudirea genetică a acestor tulpini. Din 106 tulpini rezistente la carbapeneme, 50 au dat rezultate pozitive la screening-ul prin Etest pentru producerea de MBL. Analiza PCR a arătat ca 46 dintre izolate (44 P. aeruginosa si 2 A. baumannii) aveau gena blaVIM. La tulpinile de P. aeruginosa, secvenţierea a demonstrat prezenţa a doi integroni de clasa 1 care conţin gena blaVIM: IntI1-aacA7-blaVIM-2-qacEΔ1, la 43 tulpini, respectiv IntI1-aacA7-ΔblaVIM-ΔcmlA1- qacEΔ1, la o singură tulpină. La tulpinile de A. baumannii gena blaVIM-2 nu a fost detectată într-un integron de clasa 1. Tiparea prin RAPD (Random amplified polymorphic DNA typing) a tulpinilor de P. aeruginosa producătoare de VIM-2 a evidenţiat prezenţa unui tip RAPD major, în toate cele cinci spitale. Detectarea precoce şi supravegherea unor astfel de izolate trebuie insoţită de măsuri riguroase de control al infecţiei, în vederea limitării răspândirii MBL în unitatile spitalicesti</abstract><cop>Târgu Mureș</cop><pub>Versita</pub><doi>10.2478/rrlm-2013-0035</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acinetobacter baumannii metallo-beta-lactamase metalo-beta-lactamaze Pseudomonas aeruginosa VIM-2 |
| title | Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania |
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