Comparison of mice from two different vendors for dry eye disease mouse model
Aims/Purpose: The purpose of the study was to compare corneal surface damage at baseline and 20 days after induction of dry eye disease (DED) in mice from two different vendors: Janvier Labs, France, and The Jackson Laboratory, USA. Methods: 120 C57BL/6JRj female mice (Janvier Labs, Saint‐Berthevin,...
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| Veröffentlicht in: | Acta ophthalmologica (Oxford, England) England), 2025-01, Vol.103 (S284), p.n/a |
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| creator | Thapa, Rubina Lappeteläinen, Birgitta Kolehmainen, Anni Koponen, Anna Mari Partanen, Päivi Lappalainen, Eerik Härkönen, Kirsti Pakarinen, Oona Varis, Sanni Haapaniemi, Anne Mari Pietikäinen, Piia Vähätupa, Maria |
| description | Aims/Purpose: The purpose of the study was to compare corneal surface damage at baseline and 20 days after induction of dry eye disease (DED) in mice from two different vendors: Janvier Labs, France, and The Jackson Laboratory, USA.
Methods: 120 C57BL/6JRj female mice (Janvier Labs, Saint‐Berthevin, France) and 120 C57BL/6J female mice (The Jackson Laboratory, Bar Harbor, ME USA) were received at our facility and corneal fluorescein staining (CFS) was performed to evaluate corneal surface damage at baseline. 40 mice with CFS score ranging from 0‐2 were selected for DED study. DED was induced in 8‐week‐old mice by administering scopolamine (500 μg/mouse/dose) subcutaneously three times per day for 20 days. Mice were housed in the dry eye inducing chamber (DEIC, 12l/min airflow and 13 % humidity on average). Mice were treated topically with Ikervis® or phosphate‐buffered saline (PBS) twice daily (5 μl/eye). Corneal surface damage was assessed using CFS on day 13 and day 20, and with rose bengal staining (RBS) on day 21.
Results: The baseline CFS score (median of 2) did not differ in mice from Janvier and Jackson. DED was successfully induced in mice from both vendors. However, Janvier mice developed more severe corneal damage on day 13 (median score 3 vs 2, respectively; p |
| doi_str_mv | 10.1111/aos.17207 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_3157031296</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3157031296</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1027-8c4631d9efe4e0e286ccf0c05a73b258006589bc6d5657818f979282e87415843</originalsourceid><addsrcrecordid>eNp1kE1PwzAMhiMEEmNw4B9E4sShW5I2Hz1OEwykoR0AiVvUpY7UqW1K0jL13xNWxA0fbEt-_Np6EbqlZEFjLAsXFlQyIs_QjErOk1QKdf7X849LdBXCgRBBhchm6GXtmq7wVXAtdhY3lQFsvWtwf3S4rKwFD22Pv6AtnQ_YOo9LP2IYIU4DFAFw44ZTLqG-Rhe2qAPc_NY5en98eFs_Jdvd5nm92iaGEiYTZTKR0jIHCxkQYEoYY4khvJDpnnEVv-Mq3xtRcsGlosrmMmeKgZIZ5SpL5-hu0u28-xwg9PrgBt_GkzqlXJKUslxE6n6ijHcheLC681VT-FFTon_c0tEtfXIrssuJPVY1jP-DerV7nTa-AWF2alI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3157031296</pqid></control><display><type>article</type><title>Comparison of mice from two different vendors for dry eye disease mouse model</title><source>Wiley Online Library - AutoHoldings Journals</source><creator>Thapa, Rubina ; Lappeteläinen, Birgitta ; Kolehmainen, Anni ; Koponen, Anna Mari ; Partanen, Päivi ; Lappalainen, Eerik ; Härkönen, Kirsti ; Pakarinen, Oona ; Varis, Sanni ; Haapaniemi, Anne Mari ; Pietikäinen, Piia ; Vähätupa, Maria</creator><creatorcontrib>Thapa, Rubina ; Lappeteläinen, Birgitta ; Kolehmainen, Anni ; Koponen, Anna Mari ; Partanen, Päivi ; Lappalainen, Eerik ; Härkönen, Kirsti ; Pakarinen, Oona ; Varis, Sanni ; Haapaniemi, Anne Mari ; Pietikäinen, Piia ; Vähätupa, Maria</creatorcontrib><description>Aims/Purpose: The purpose of the study was to compare corneal surface damage at baseline and 20 days after induction of dry eye disease (DED) in mice from two different vendors: Janvier Labs, France, and The Jackson Laboratory, USA.
Methods: 120 C57BL/6JRj female mice (Janvier Labs, Saint‐Berthevin, France) and 120 C57BL/6J female mice (The Jackson Laboratory, Bar Harbor, ME USA) were received at our facility and corneal fluorescein staining (CFS) was performed to evaluate corneal surface damage at baseline. 40 mice with CFS score ranging from 0‐2 were selected for DED study. DED was induced in 8‐week‐old mice by administering scopolamine (500 μg/mouse/dose) subcutaneously three times per day for 20 days. Mice were housed in the dry eye inducing chamber (DEIC, 12l/min airflow and 13 % humidity on average). Mice were treated topically with Ikervis® or phosphate‐buffered saline (PBS) twice daily (5 μl/eye). Corneal surface damage was assessed using CFS on day 13 and day 20, and with rose bengal staining (RBS) on day 21.
Results: The baseline CFS score (median of 2) did not differ in mice from Janvier and Jackson. DED was successfully induced in mice from both vendors. However, Janvier mice developed more severe corneal damage on day 13 (median score 3 vs 2, respectively; p < 0.05). CFS score in Jackson mice did not reach statistical difference on day 20 as compared to baseline (median score 1 vs 2, respectively; p = 0.21). Mice treated with Ikervis® did not differ from PBS‐treated mice in corneal surface damage as assessed using CFS on Day 13 and 20 and RBS on Day 21. RBS staining results from PBS‐treated mice were similar in Janvier and Jackson mice.
Conclusions: The corneal damage at the baseline was similar in both vendors’ mice. The successful induction of dry eye disease was evident in both vendors’ mice based on day 13 CFS data. However, the phenotype persisted only with Janvier mice. This dosing regimen of Ikervis® failed to show any therapeutic effect as assessed from CFS and RBS scoring in mice from either of the vendors.</description><identifier>ISSN: 1755-375X</identifier><identifier>EISSN: 1755-3768</identifier><identifier>DOI: 10.1111/aos.17207</identifier><language>eng</language><publisher>Malden: Wiley Subscription Services, Inc</publisher><subject>Cornea ; Dosage ; Eye diseases ; Phenotypes ; Scopolamine</subject><ispartof>Acta ophthalmologica (Oxford, England), 2025-01, Vol.103 (S284), p.n/a</ispartof><rights>2025 The Authors Acta Ophthalmologica © 2025 Acta Ophthalmologica Scandinavica Foundation</rights><rights>Copyright © 2025 Acta Ophthalmologica Scandinavica Foundation</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faos.17207$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45551</link.rule.ids></links><search><creatorcontrib>Thapa, Rubina</creatorcontrib><creatorcontrib>Lappeteläinen, Birgitta</creatorcontrib><creatorcontrib>Kolehmainen, Anni</creatorcontrib><creatorcontrib>Koponen, Anna Mari</creatorcontrib><creatorcontrib>Partanen, Päivi</creatorcontrib><creatorcontrib>Lappalainen, Eerik</creatorcontrib><creatorcontrib>Härkönen, Kirsti</creatorcontrib><creatorcontrib>Pakarinen, Oona</creatorcontrib><creatorcontrib>Varis, Sanni</creatorcontrib><creatorcontrib>Haapaniemi, Anne Mari</creatorcontrib><creatorcontrib>Pietikäinen, Piia</creatorcontrib><creatorcontrib>Vähätupa, Maria</creatorcontrib><title>Comparison of mice from two different vendors for dry eye disease mouse model</title><title>Acta ophthalmologica (Oxford, England)</title><description>Aims/Purpose: The purpose of the study was to compare corneal surface damage at baseline and 20 days after induction of dry eye disease (DED) in mice from two different vendors: Janvier Labs, France, and The Jackson Laboratory, USA.
Methods: 120 C57BL/6JRj female mice (Janvier Labs, Saint‐Berthevin, France) and 120 C57BL/6J female mice (The Jackson Laboratory, Bar Harbor, ME USA) were received at our facility and corneal fluorescein staining (CFS) was performed to evaluate corneal surface damage at baseline. 40 mice with CFS score ranging from 0‐2 were selected for DED study. DED was induced in 8‐week‐old mice by administering scopolamine (500 μg/mouse/dose) subcutaneously three times per day for 20 days. Mice were housed in the dry eye inducing chamber (DEIC, 12l/min airflow and 13 % humidity on average). Mice were treated topically with Ikervis® or phosphate‐buffered saline (PBS) twice daily (5 μl/eye). Corneal surface damage was assessed using CFS on day 13 and day 20, and with rose bengal staining (RBS) on day 21.
Results: The baseline CFS score (median of 2) did not differ in mice from Janvier and Jackson. DED was successfully induced in mice from both vendors. However, Janvier mice developed more severe corneal damage on day 13 (median score 3 vs 2, respectively; p < 0.05). CFS score in Jackson mice did not reach statistical difference on day 20 as compared to baseline (median score 1 vs 2, respectively; p = 0.21). Mice treated with Ikervis® did not differ from PBS‐treated mice in corneal surface damage as assessed using CFS on Day 13 and 20 and RBS on Day 21. RBS staining results from PBS‐treated mice were similar in Janvier and Jackson mice.
Conclusions: The corneal damage at the baseline was similar in both vendors’ mice. The successful induction of dry eye disease was evident in both vendors’ mice based on day 13 CFS data. However, the phenotype persisted only with Janvier mice. This dosing regimen of Ikervis® failed to show any therapeutic effect as assessed from CFS and RBS scoring in mice from either of the vendors.</description><subject>Cornea</subject><subject>Dosage</subject><subject>Eye diseases</subject><subject>Phenotypes</subject><subject>Scopolamine</subject><issn>1755-375X</issn><issn>1755-3768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><recordid>eNp1kE1PwzAMhiMEEmNw4B9E4sShW5I2Hz1OEwykoR0AiVvUpY7UqW1K0jL13xNWxA0fbEt-_Np6EbqlZEFjLAsXFlQyIs_QjErOk1QKdf7X849LdBXCgRBBhchm6GXtmq7wVXAtdhY3lQFsvWtwf3S4rKwFD22Pv6AtnQ_YOo9LP2IYIU4DFAFw44ZTLqG-Rhe2qAPc_NY5en98eFs_Jdvd5nm92iaGEiYTZTKR0jIHCxkQYEoYY4khvJDpnnEVv-Mq3xtRcsGlosrmMmeKgZIZ5SpL5-hu0u28-xwg9PrgBt_GkzqlXJKUslxE6n6ijHcheLC681VT-FFTon_c0tEtfXIrssuJPVY1jP-DerV7nTa-AWF2alI</recordid><startdate>202501</startdate><enddate>202501</enddate><creator>Thapa, Rubina</creator><creator>Lappeteläinen, Birgitta</creator><creator>Kolehmainen, Anni</creator><creator>Koponen, Anna Mari</creator><creator>Partanen, Päivi</creator><creator>Lappalainen, Eerik</creator><creator>Härkönen, Kirsti</creator><creator>Pakarinen, Oona</creator><creator>Varis, Sanni</creator><creator>Haapaniemi, Anne Mari</creator><creator>Pietikäinen, Piia</creator><creator>Vähätupa, Maria</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>202501</creationdate><title>Comparison of mice from two different vendors for dry eye disease mouse model</title><author>Thapa, Rubina ; Lappeteläinen, Birgitta ; Kolehmainen, Anni ; Koponen, Anna Mari ; Partanen, Päivi ; Lappalainen, Eerik ; Härkönen, Kirsti ; Pakarinen, Oona ; Varis, Sanni ; Haapaniemi, Anne Mari ; Pietikäinen, Piia ; Vähätupa, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1027-8c4631d9efe4e0e286ccf0c05a73b258006589bc6d5657818f979282e87415843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Cornea</topic><topic>Dosage</topic><topic>Eye diseases</topic><topic>Phenotypes</topic><topic>Scopolamine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thapa, Rubina</creatorcontrib><creatorcontrib>Lappeteläinen, Birgitta</creatorcontrib><creatorcontrib>Kolehmainen, Anni</creatorcontrib><creatorcontrib>Koponen, Anna Mari</creatorcontrib><creatorcontrib>Partanen, Päivi</creatorcontrib><creatorcontrib>Lappalainen, Eerik</creatorcontrib><creatorcontrib>Härkönen, Kirsti</creatorcontrib><creatorcontrib>Pakarinen, Oona</creatorcontrib><creatorcontrib>Varis, Sanni</creatorcontrib><creatorcontrib>Haapaniemi, Anne Mari</creatorcontrib><creatorcontrib>Pietikäinen, Piia</creatorcontrib><creatorcontrib>Vähätupa, Maria</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Acta ophthalmologica (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thapa, Rubina</au><au>Lappeteläinen, Birgitta</au><au>Kolehmainen, Anni</au><au>Koponen, Anna Mari</au><au>Partanen, Päivi</au><au>Lappalainen, Eerik</au><au>Härkönen, Kirsti</au><au>Pakarinen, Oona</au><au>Varis, Sanni</au><au>Haapaniemi, Anne Mari</au><au>Pietikäinen, Piia</au><au>Vähätupa, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of mice from two different vendors for dry eye disease mouse model</atitle><jtitle>Acta ophthalmologica (Oxford, England)</jtitle><date>2025-01</date><risdate>2025</risdate><volume>103</volume><issue>S284</issue><epage>n/a</epage><issn>1755-375X</issn><eissn>1755-3768</eissn><abstract>Aims/Purpose: The purpose of the study was to compare corneal surface damage at baseline and 20 days after induction of dry eye disease (DED) in mice from two different vendors: Janvier Labs, France, and The Jackson Laboratory, USA.
Methods: 120 C57BL/6JRj female mice (Janvier Labs, Saint‐Berthevin, France) and 120 C57BL/6J female mice (The Jackson Laboratory, Bar Harbor, ME USA) were received at our facility and corneal fluorescein staining (CFS) was performed to evaluate corneal surface damage at baseline. 40 mice with CFS score ranging from 0‐2 were selected for DED study. DED was induced in 8‐week‐old mice by administering scopolamine (500 μg/mouse/dose) subcutaneously three times per day for 20 days. Mice were housed in the dry eye inducing chamber (DEIC, 12l/min airflow and 13 % humidity on average). Mice were treated topically with Ikervis® or phosphate‐buffered saline (PBS) twice daily (5 μl/eye). Corneal surface damage was assessed using CFS on day 13 and day 20, and with rose bengal staining (RBS) on day 21.
Results: The baseline CFS score (median of 2) did not differ in mice from Janvier and Jackson. DED was successfully induced in mice from both vendors. However, Janvier mice developed more severe corneal damage on day 13 (median score 3 vs 2, respectively; p < 0.05). CFS score in Jackson mice did not reach statistical difference on day 20 as compared to baseline (median score 1 vs 2, respectively; p = 0.21). Mice treated with Ikervis® did not differ from PBS‐treated mice in corneal surface damage as assessed using CFS on Day 13 and 20 and RBS on Day 21. RBS staining results from PBS‐treated mice were similar in Janvier and Jackson mice.
Conclusions: The corneal damage at the baseline was similar in both vendors’ mice. The successful induction of dry eye disease was evident in both vendors’ mice based on day 13 CFS data. However, the phenotype persisted only with Janvier mice. This dosing regimen of Ikervis® failed to show any therapeutic effect as assessed from CFS and RBS scoring in mice from either of the vendors.</abstract><cop>Malden</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/aos.17207</doi><tpages>1</tpages></addata></record> |
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| subjects | Cornea Dosage Eye diseases Phenotypes Scopolamine |
| title | Comparison of mice from two different vendors for dry eye disease mouse model |
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