Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections
Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous my...
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| Veröffentlicht in: | Lasers in medical science 2025-01, Vol.40 (1), p.9, Article 9 |
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| creator | Dang, Yang Yang, Runping Jia, Tao Liu, Chengcheng Geng, Songmei |
| description | Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous mycobacteria,
Mycobacterium abscessus
, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against
M.abscessus
ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using H
2
DCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on
M.abscessus
in a concentration- and light-dose-dependent manner. Complete inactivation of
M.abscessus
was achieved with 100 μM curcumin and 48 J cm
−
2
light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5.32 and 9.53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that
M.abscessus
could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for
M.abscessus
. |
| doi_str_mv | 10.1007/s10103-024-04263-0 |
| format | Article |
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Mycobacterium abscessus
, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against
M.abscessus
ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using H
2
DCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on
M.abscessus
in a concentration- and light-dose-dependent manner. Complete inactivation of
M.abscessus
was achieved with 100 μM curcumin and 48 J cm
−
2
light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5.32 and 9.53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that
M.abscessus
could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for
M.abscessus
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Mycobacterium abscessus
, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against
M.abscessus
ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using H
2
DCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on
M.abscessus
in a concentration- and light-dose-dependent manner. Complete inactivation of
M.abscessus
was achieved with 100 μM curcumin and 48 J cm
−
2
light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5.32 and 9.53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that
M.abscessus
could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for
M.abscessus
.</description><subject>Antimicrobial activity</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>Curcumin</subject><subject>Damage</subject><subject>Deactivation</subject><subject>Dentistry</subject><subject>Dermatology</subject><subject>Iodides</subject><subject>Lasers</subject><subject>Luminous intensity</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mycobacterium abscessus</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photodynamic therapy</subject><subject>Photonics</subject><subject>Propidium iodide</subject><subject>Quantum Optics</subject><subject>Reactive oxygen species</subject><subject>Transmission electron microscopy</subject><subject>Tuberculosis</subject><issn>1435-604X</issn><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><recordid>eNp9UctKxDAULaKgjv6Aq4Dr6k3TtFN3MvgCxY2Cu3CbhxOdJjVJhfkg_9PoCLpydc8N53HJKYojCicUoD2NFCiwEqq6hLpqMtoq9mjNeNlA_bT9B-8W-zG-ANC2oWyv-FhMQU6DdeWglcWkFUGX7GBl8L3FFRmXPnm1dpifSFrqgOOaGB-IdSiTfcdk3TO5W0vf510HOw0E-yh1jFM8I0jG4Acbv0g4Zoxy-S133pVp6nVOX_kpkuHXIafGV-tygtE5wrt4UOwYXEV9-DNnxePlxcPiury9v7pZnN-WsgJIZVejVh3yDhXymjcGDGbPOZNtpZhpK6YYn0uOLccKaaOo4dCi4mbeU5CczYrjjW8-9G3SMYkXPwWXIwWjnHZ101VtZlUbVv6jGIM2Ygx2wLAWFMRXHWJTh8h1iO86BGQR24hiJrtnHX6t_1F9AmIHk90</recordid><startdate>20250107</startdate><enddate>20250107</enddate><creator>Dang, Yang</creator><creator>Yang, Runping</creator><creator>Jia, Tao</creator><creator>Liu, Chengcheng</creator><creator>Geng, Songmei</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>H8D</scope><scope>K9.</scope><scope>L7M</scope><scope>NAPCQ</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0001-8731-6267</orcidid></search><sort><creationdate>20250107</creationdate><title>Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections</title><author>Dang, Yang ; Yang, Runping ; Jia, Tao ; Liu, Chengcheng ; Geng, Songmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c200t-94aed9a59ada5456f0fabac83c72d3f723d358c5a75a2a16d1f507ad5f8b10c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Antimicrobial activity</topic><topic>Cell membranes</topic><topic>Cell walls</topic><topic>Curcumin</topic><topic>Damage</topic><topic>Deactivation</topic><topic>Dentistry</topic><topic>Dermatology</topic><topic>Iodides</topic><topic>Lasers</topic><topic>Luminous intensity</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mycobacterium abscessus</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photodynamic therapy</topic><topic>Photonics</topic><topic>Propidium iodide</topic><topic>Quantum Optics</topic><topic>Reactive oxygen species</topic><topic>Transmission electron microscopy</topic><topic>Tuberculosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dang, Yang</creatorcontrib><creatorcontrib>Yang, Runping</creatorcontrib><creatorcontrib>Jia, Tao</creatorcontrib><creatorcontrib>Liu, Chengcheng</creatorcontrib><creatorcontrib>Geng, Songmei</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dang, Yang</au><au>Yang, Runping</au><au>Jia, Tao</au><au>Liu, Chengcheng</au><au>Geng, Songmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><date>2025-01-07</date><risdate>2025</risdate><volume>40</volume><issue>1</issue><spage>9</spage><pages>9-</pages><artnum>9</artnum><issn>1435-604X</issn><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous mycobacteria,
Mycobacterium abscessus
, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against
M.abscessus
ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using H
2
DCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on
M.abscessus
in a concentration- and light-dose-dependent manner. Complete inactivation of
M.abscessus
was achieved with 100 μM curcumin and 48 J cm
−
2
light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5.32 and 9.53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that
M.abscessus
could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for
M.abscessus
.</abstract><cop>London</cop><pub>Springer London</pub><doi>10.1007/s10103-024-04263-0</doi><orcidid>https://orcid.org/0000-0001-8731-6267</orcidid></addata></record> |
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| subjects | Antimicrobial activity Cell membranes Cell walls Curcumin Damage Deactivation Dentistry Dermatology Iodides Lasers Luminous intensity Medicine Medicine & Public Health Mycobacterium abscessus Optical Devices Optics Original Article Photodynamic therapy Photonics Propidium iodide Quantum Optics Reactive oxygen species Transmission electron microscopy Tuberculosis |
| title | Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections |
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