Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep
Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it indu...
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| Veröffentlicht in: | Animal biotechnology 2024-11, Vol.35 (1), p.2269428-2269428 |
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| creator | Singh, Rohit Singh, Karam Pal Singh, Rajendra Singh, Vidya Kumar, Pawan Varshney, Rajat Yadav, Akanksha Mote, Akash Gangwar, Mukesh Prasath, N. Babu |
| description | Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7
th
days postinoculation (DPI) and in thyroid and pituitary glands at 15
th
DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals. |
| doi_str_mv | 10.1080/10495398.2023.2269428 |
| format | Article |
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th
days postinoculation (DPI) and in thyroid and pituitary glands at 15
th
DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals.</description><identifier>ISSN: 1049-5398</identifier><identifier>EISSN: 1532-2378</identifier><identifier>DOI: 10.1080/10495398.2023.2269428</identifier><identifier>PMID: 37850824</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Adrenal glands ; Animals ; Antigens ; Apoptosis ; Bluetongue ; Bluetongue - diagnosis ; Bluetongue virus ; BTV-1 ; Caspase-3 ; Endocrine glands ; Endocrine Glands - pathology ; Epithelial cells ; Epithelium ; Female ; histopathology ; Immunohistochemistry ; Indian local pregnant sheep ; Load distribution ; Pathogenesis ; Pituitary (anterior) ; Pregnancy ; Real time ; real-time PCR ; Sheep ; Sheep Diseases ; Thyroid ; Thyroid gland ; Tropism</subject><ispartof>Animal biotechnology, 2024-11, Vol.35 (1), p.2269428-2269428</ispartof><rights>2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2023</rights><rights>2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c455t-434cb11831840a52caa2395f8922442d29790b56adbda93ab717b3f87c0a887d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,862,2098,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37850824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Rohit</creatorcontrib><creatorcontrib>Singh, Karam Pal</creatorcontrib><creatorcontrib>Singh, Rajendra</creatorcontrib><creatorcontrib>Singh, Vidya</creatorcontrib><creatorcontrib>Kumar, Pawan</creatorcontrib><creatorcontrib>Varshney, Rajat</creatorcontrib><creatorcontrib>Yadav, Akanksha</creatorcontrib><creatorcontrib>Mote, Akash</creatorcontrib><creatorcontrib>Gangwar, Mukesh</creatorcontrib><creatorcontrib>Prasath, N. Babu</creatorcontrib><title>Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep</title><title>Animal biotechnology</title><addtitle>Anim Biotechnol</addtitle><description>Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7
th
days postinoculation (DPI) and in thyroid and pituitary glands at 15
th
DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals.</description><subject>Adrenal glands</subject><subject>Animals</subject><subject>Antigens</subject><subject>Apoptosis</subject><subject>Bluetongue</subject><subject>Bluetongue - diagnosis</subject><subject>Bluetongue virus</subject><subject>BTV-1</subject><subject>Caspase-3</subject><subject>Endocrine glands</subject><subject>Endocrine Glands - pathology</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Female</subject><subject>histopathology</subject><subject>Immunohistochemistry</subject><subject>Indian local pregnant sheep</subject><subject>Load distribution</subject><subject>Pathogenesis</subject><subject>Pituitary (anterior)</subject><subject>Pregnancy</subject><subject>Real time</subject><subject>real-time PCR</subject><subject>Sheep</subject><subject>Sheep Diseases</subject><subject>Thyroid</subject><subject>Thyroid gland</subject><subject>Tropism</subject><issn>1049-5398</issn><issn>1532-2378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNp9ksuOFCEUhitG44yjj6AhceOmWq4D7DQTL51Mogtdk1NAVTOhoYWqNvMaPrH0ZWbhwhUEvvOf2991rwleEazwe4K5FkyrFcWUrSi91pyqJ90lEYz2lEn1tN0b0x-gi-5FrXcYE6oYft5dtG-BFeWX3Z_vxcewDQnKPQpp7-scJphDTqj4vYdYUcp7H9EO5k2OeQoWIrI5Vf9r8cn6ivKIhrj4Oadp8WgfylJ70rRGb486IaF545FPLtsSkkdThOSOcbvipwRpRuvkAiRUN97vXnbPxpbXvzqfV93Pz59-3Hztb799Wd98vO0tF2LuOeN2IEQxojgGQS0AZVqMSlPKOXVUS40HcQ1ucKAZDJLIgY1KWgxKSceuuvVJ12W4M7sStm0GJkMwx4dcJgNlDjZ6A86NQipCBQeu8Ki1okRIbp1iUgtoWu9OWruS21zqbLahWh9bpz4v1VAllSRYEt3Qt_-gd3kpqXVqGBGYC8aPlDhRtuRaix8fCyTYHAxgHgxgDgYwZwO0uDdn9WXYevcY9bDxBnw4AW1BuWzhdy7RmRnuYy5jgWTDoY7_5vgLAHPAtQ</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Singh, Rohit</creator><creator>Singh, Karam Pal</creator><creator>Singh, Rajendra</creator><creator>Singh, Vidya</creator><creator>Kumar, Pawan</creator><creator>Varshney, Rajat</creator><creator>Yadav, Akanksha</creator><creator>Mote, Akash</creator><creator>Gangwar, Mukesh</creator><creator>Prasath, N. 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Babu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep</atitle><jtitle>Animal biotechnology</jtitle><addtitle>Anim Biotechnol</addtitle><date>2024-11</date><risdate>2024</risdate><volume>35</volume><issue>1</issue><spage>2269428</spage><epage>2269428</epage><pages>2269428-2269428</pages><issn>1049-5398</issn><eissn>1532-2378</eissn><abstract>Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7
th
days postinoculation (DPI) and in thyroid and pituitary glands at 15
th
DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>37850824</pmid><doi>10.1080/10495398.2023.2269428</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Animal biotechnology, 2024-11, Vol.35 (1), p.2269428-2269428 |
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| subjects | Adrenal glands Animals Antigens Apoptosis Bluetongue Bluetongue - diagnosis Bluetongue virus BTV-1 Caspase-3 Endocrine glands Endocrine Glands - pathology Epithelial cells Epithelium Female histopathology Immunohistochemistry Indian local pregnant sheep Load distribution Pathogenesis Pituitary (anterior) Pregnancy Real time real-time PCR Sheep Sheep Diseases Thyroid Thyroid gland Tropism |
| title | Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep |
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