Directed Mutagenesis for Arginine Substitution of a Phaseolus acutifolius Recombinant Lectin Disrupts Its Cytotoxic Activity

Recently, we reported that a recombinant Tepary bean ( ) lectin (rTBL-1) induces apoptosis in colon cancer cell lines and that cytotoxicity was related to differential recognition of β1-6 branched -glycans. Sequencing analysis and resolution of the rTBL-1 3D structure suggest that glycan specificity...

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Veröffentlicht in:	International journal of molecular sciences 2024-12, Vol.25 (24), p.13258
Hauptverfasser:	Martínez-Alarcón, Dania, Castro-Guillén, José Luis, Fitches, Elaine, Gatehouse, John A, Przyborski, Stefan, Moreno-Celis, Ulisses, Blanco-Labra, Alejandro, García-Gasca, Teresa
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Analysis Apoptosis Arginine Arginine - chemistry Carbohydrates Cell cycle Chromatography Colon cancer Colorectal cancer Cytotoxicity Epidermal growth factor ErbB Receptors - chemistry ErbB Receptors - genetics ErbB Receptors - metabolism Fermentation HT29 Cells Humans Lectins Legumes Molecular Docking Simulation Molecular weight Mutagenesis Mutagenesis, Site-Directed Phaseolus - genetics Plant Lectins - chemistry Plant Lectins - genetics Plant Lectins - pharmacology Polysaccharides Polysaccharides - chemistry Polysaccharides - pharmacology Protein Binding Proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism
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