Examining the response of Botrytis cinerea to aqueous ozone: inactivation kinetics, structure, physiology, and growth in different strawberries cultivars
This work aimed to study the kinetics and mechanisms of Botrytis cinerea conidia inactivation by aqueous ozone treatments (2.5 ± 0.8 mg O 3 L −1 and 4.2 ± 0.2 mg O 3 L −1 , 0.5-16 min), using conventional plate count technique, flow cytometry analysis (FCM), and transmission electron microscopy (TEM...
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| creator | Romero Bernal, Angela Raffellini, Silvia Jaramillo-Sánchez, Gabriela Alzamora, Stella Maris Gómez, Paula Luisina |
| description | This work aimed to study the kinetics and mechanisms of Botrytis cinerea conidia inactivation by aqueous ozone treatments (2.5 ± 0.8 mg O
3
L
−1
and 4.2 ± 0.2 mg O
3
L
−1
, 0.5-16 min), using conventional plate count technique, flow cytometry analysis (FCM), and transmission electron microscopy (TEM). The effect of treatments on the mold incidence on two artificially contaminated strawberries cultivars over refrigerated storage (6 ± 1 °C) was also investigated. The inactivation pattern of B. cinerea conidial suspensions depended on initial level of the microorganism, dissolved ozone concentration and treatment time. Non-linear survival curves were obtained and fitted successfully with the Weibullian model. FCM and TEM analysis revealed alterations in the physiological state and ultrastructure of ozonated conidia that depended on the ozone dose. Ozonation (4.4 mg O
3
L
−1
, 5-10 min) reduced B. cinerea incidence throughout storage in strawberries of cv. Fortuna, but no effect was found in cv. Aroma. |
| doi_str_mv | 10.1080/19476337.2024.2339446 |
| format | Article |
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3
L
−1
and 4.2 ± 0.2 mg O
3
L
−1
, 0.5-16 min), using conventional plate count technique, flow cytometry analysis (FCM), and transmission electron microscopy (TEM). The effect of treatments on the mold incidence on two artificially contaminated strawberries cultivars over refrigerated storage (6 ± 1 °C) was also investigated. The inactivation pattern of B. cinerea conidial suspensions depended on initial level of the microorganism, dissolved ozone concentration and treatment time. Non-linear survival curves were obtained and fitted successfully with the Weibullian model. FCM and TEM analysis revealed alterations in the physiological state and ultrastructure of ozonated conidia that depended on the ozone dose. Ozonation (4.4 mg O
3
L
−1
, 5-10 min) reduced B. cinerea incidence throughout storage in strawberries of cv. Fortuna, but no effect was found in cv. Aroma.</description><identifier>ISSN: 1947-6337</identifier><identifier>EISSN: 1947-6345</identifier><identifier>DOI: 10.1080/19476337.2024.2339446</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>aqueous ozone ; Aroma ; Berries ; Botrytis cinerea ; Cold storage ; Conidia ; Cultivars ; Deactivation ; decay ; Flow cytometry ; Inactivation ; Kinetics ; Ozonation ; Ozone ; Physiological effects ; Physiology ; single conidia analysis ; Strawberries ; strawberry ; Transmission electron microscopy ; Ultrastructure</subject><ispartof>CYTA: journal of food, 2024-12, Vol.22 (1)</ispartof><rights>2024 Buenos Aires University- National Scientific and Technical Research Council Argentina. Published with license by Taylor & Francis Group, LLC. 2024</rights><rights>2024 Buenos Aires University- National Scientific and Technical Research Council Argentina. Published with license by Taylor & Francis Group, LLC. This work is licensed under the Creative Commons Attribution – Non-Commercial License http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c399t-c3648b12786560f0bbc518c9b32b39268939ba6ccf2272b11864be5e3931687a3</cites><orcidid>0000-0001-5865-6561 ; 0000-0003-3630-042X ; 0000-0001-9026-4912 ; 0000-0001-9128-1984</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/19476337.2024.2339446$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/19476337.2024.2339446$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,2100,27500,27922,27923,59141,59142</link.rule.ids></links><search><creatorcontrib>Romero Bernal, Angela</creatorcontrib><creatorcontrib>Raffellini, Silvia</creatorcontrib><creatorcontrib>Jaramillo-Sánchez, Gabriela</creatorcontrib><creatorcontrib>Alzamora, Stella Maris</creatorcontrib><creatorcontrib>Gómez, Paula Luisina</creatorcontrib><title>Examining the response of Botrytis cinerea to aqueous ozone: inactivation kinetics, structure, physiology, and growth in different strawberries cultivars</title><title>CYTA: journal of food</title><description>This work aimed to study the kinetics and mechanisms of Botrytis cinerea conidia inactivation by aqueous ozone treatments (2.5 ± 0.8 mg O
3
L
−1
and 4.2 ± 0.2 mg O
3
L
−1
, 0.5-16 min), using conventional plate count technique, flow cytometry analysis (FCM), and transmission electron microscopy (TEM). The effect of treatments on the mold incidence on two artificially contaminated strawberries cultivars over refrigerated storage (6 ± 1 °C) was also investigated. The inactivation pattern of B. cinerea conidial suspensions depended on initial level of the microorganism, dissolved ozone concentration and treatment time. Non-linear survival curves were obtained and fitted successfully with the Weibullian model. FCM and TEM analysis revealed alterations in the physiological state and ultrastructure of ozonated conidia that depended on the ozone dose. Ozonation (4.4 mg O
3
L
−1
, 5-10 min) reduced B. cinerea incidence throughout storage in strawberries of cv. Fortuna, but no effect was found in cv. Aroma.</description><subject>aqueous ozone</subject><subject>Aroma</subject><subject>Berries</subject><subject>Botrytis cinerea</subject><subject>Cold storage</subject><subject>Conidia</subject><subject>Cultivars</subject><subject>Deactivation</subject><subject>decay</subject><subject>Flow cytometry</subject><subject>Inactivation</subject><subject>Kinetics</subject><subject>Ozonation</subject><subject>Ozone</subject><subject>Physiological effects</subject><subject>Physiology</subject><subject>single conidia analysis</subject><subject>Strawberries</subject><subject>strawberry</subject><subject>Transmission electron microscopy</subject><subject>Ultrastructure</subject><issn>1947-6337</issn><issn>1947-6345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNp9kVGP1CAUhRujievqTzAh8XVmlkJLqU_qZl032cQXfSYXCjOMHRgv1LH-E_-t1Fn30RcgN-d8BzhV9bqmm5pKelX3TSc47zaMsmbDOO-bRjypLpb5WvCmffp45t3z6kVKe0pF2zFxUf2--QkHH3zYkryzBG06xpAsiY58iBnn7BMxPli0QHIk8H2ycUok_orBviU-gMn-B2QfA_lWZNmbtCIp42TyhHZFjrs5-TjG7bwiEAayxXjKu2Ikg3euYENe5HDSFtHbEjaNCxHTy-qZgzHZVw_7ZfX1482X60_r-8-3d9fv79eG930uq2ikrlknRSuoo1qbtpam15xp3jMhe95rEMY4xjqm61qKRtvW8p7XQnbAL6u7M3eIsFdH9AfAWUXw6u8g4lYBlneNVnWgBzlIp3ugDTMGBjpIyaWDAQxjrLDenFlHjOWnUlb7OGEo11e8boRoBJO8qNqzymBMCa17TK2pWhpV_xpVS6PqodHie3f2-eAiHuAUcRxUhnmM6BCC8UvMfxF_AAOjq3w</recordid><startdate>20241231</startdate><enddate>20241231</enddate><creator>Romero Bernal, Angela</creator><creator>Raffellini, Silvia</creator><creator>Jaramillo-Sánchez, Gabriela</creator><creator>Alzamora, Stella Maris</creator><creator>Gómez, Paula Luisina</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7T7</scope><scope>7U7</scope><scope>7X2</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M2O</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5865-6561</orcidid><orcidid>https://orcid.org/0000-0003-3630-042X</orcidid><orcidid>https://orcid.org/0000-0001-9026-4912</orcidid><orcidid>https://orcid.org/0000-0001-9128-1984</orcidid></search><sort><creationdate>20241231</creationdate><title>Examining the response of Botrytis cinerea to aqueous ozone: inactivation kinetics, structure, physiology, and growth in different strawberries cultivars</title><author>Romero Bernal, Angela ; Raffellini, Silvia ; Jaramillo-Sánchez, Gabriela ; Alzamora, Stella Maris ; Gómez, Paula Luisina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-c3648b12786560f0bbc518c9b32b39268939ba6ccf2272b11864be5e3931687a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>aqueous ozone</topic><topic>Aroma</topic><topic>Berries</topic><topic>Botrytis cinerea</topic><topic>Cold storage</topic><topic>Conidia</topic><topic>Cultivars</topic><topic>Deactivation</topic><topic>decay</topic><topic>Flow cytometry</topic><topic>Inactivation</topic><topic>Kinetics</topic><topic>Ozonation</topic><topic>Ozone</topic><topic>Physiological effects</topic><topic>Physiology</topic><topic>single conidia analysis</topic><topic>Strawberries</topic><topic>strawberry</topic><topic>Transmission electron microscopy</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Romero Bernal, Angela</creatorcontrib><creatorcontrib>Raffellini, Silvia</creatorcontrib><creatorcontrib>Jaramillo-Sánchez, Gabriela</creatorcontrib><creatorcontrib>Alzamora, Stella Maris</creatorcontrib><creatorcontrib>Gómez, Paula Luisina</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Research Library</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>CYTA: journal of food</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Romero Bernal, Angela</au><au>Raffellini, Silvia</au><au>Jaramillo-Sánchez, Gabriela</au><au>Alzamora, Stella Maris</au><au>Gómez, Paula Luisina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Examining the response of Botrytis cinerea to aqueous ozone: inactivation kinetics, structure, physiology, and growth in different strawberries cultivars</atitle><jtitle>CYTA: journal of food</jtitle><date>2024-12-31</date><risdate>2024</risdate><volume>22</volume><issue>1</issue><issn>1947-6337</issn><eissn>1947-6345</eissn><abstract>This work aimed to study the kinetics and mechanisms of Botrytis cinerea conidia inactivation by aqueous ozone treatments (2.5 ± 0.8 mg O
3
L
−1
and 4.2 ± 0.2 mg O
3
L
−1
, 0.5-16 min), using conventional plate count technique, flow cytometry analysis (FCM), and transmission electron microscopy (TEM). The effect of treatments on the mold incidence on two artificially contaminated strawberries cultivars over refrigerated storage (6 ± 1 °C) was also investigated. The inactivation pattern of B. cinerea conidial suspensions depended on initial level of the microorganism, dissolved ozone concentration and treatment time. Non-linear survival curves were obtained and fitted successfully with the Weibullian model. FCM and TEM analysis revealed alterations in the physiological state and ultrastructure of ozonated conidia that depended on the ozone dose. Ozonation (4.4 mg O
3
L
−1
, 5-10 min) reduced B. cinerea incidence throughout storage in strawberries of cv. Fortuna, but no effect was found in cv. Aroma.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/19476337.2024.2339446</doi><orcidid>https://orcid.org/0000-0001-5865-6561</orcidid><orcidid>https://orcid.org/0000-0003-3630-042X</orcidid><orcidid>https://orcid.org/0000-0001-9026-4912</orcidid><orcidid>https://orcid.org/0000-0001-9128-1984</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; Alma/SFX Local Collection |
| subjects | aqueous ozone Aroma Berries Botrytis cinerea Cold storage Conidia Cultivars Deactivation decay Flow cytometry Inactivation Kinetics Ozonation Ozone Physiological effects Physiology single conidia analysis Strawberries strawberry Transmission electron microscopy Ultrastructure |
| title | Examining the response of Botrytis cinerea to aqueous ozone: inactivation kinetics, structure, physiology, and growth in different strawberries cultivars |
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