Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing
This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfect...
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| Veröffentlicht in: | CYTA: journal of food 2024-12, Vol.22 (1) |
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| creator | He, Miao Ma, Lingyan Lv, Wentao Xiao, Yingping Yang, Hua Wang, Xiaofan Zhao, Jiangchao Lan, Hangzhen Wang, Wen Xiao, Xingning |
| description | This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p |
| doi_str_mv | 10.1080/19476337.2024.2351906 |
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In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p < .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p < .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection altered the composition of bacterial communities.</description><identifier>ISSN: 1947-6337</identifier><identifier>EISSN: 1947-6345</identifier><identifier>DOI: 10.1080/19476337.2024.2351906</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>16S rDNA amplicon sequencing ; bacterial contamination ; Community composition ; Composition ; Contamination ; Cutlery ; Decontamination ; Discriminant analysis ; Disinfectants ; Disinfection ; Effectiveness ; Food contamination ; Hooks ; Knives ; Meat ; Microbial contamination ; Microbiomes ; Microorganisms ; NaClO disinfection ; Pork ; rRNA 16S ; Sodium hypochlorite ; Species richness ; Transport vehicles ; wholesaler</subject><ispartof>CYTA: journal of food, 2024-12, Vol.22 (1)</ispartof><rights>2024 The Author(s). Published with license by Taylor & Francis Group, LLC. 2024</rights><rights>2024 The Author(s). Published with license by Taylor & Francis Group, LLC. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-20b63a886e45e2d205b3664db520bae26b498031b9f0ce440cce16b815e071e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/19476337.2024.2351906$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/19476337.2024.2351906$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,864,885,2102,27502,27924,27925,59143,59144</link.rule.ids><backlink>$$Uhttps://www.osti.gov/biblio/2352527$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Miao</creatorcontrib><creatorcontrib>Ma, Lingyan</creatorcontrib><creatorcontrib>Lv, Wentao</creatorcontrib><creatorcontrib>Xiao, Yingping</creatorcontrib><creatorcontrib>Yang, Hua</creatorcontrib><creatorcontrib>Wang, Xiaofan</creatorcontrib><creatorcontrib>Zhao, Jiangchao</creatorcontrib><creatorcontrib>Lan, Hangzhen</creatorcontrib><creatorcontrib>Wang, Wen</creatorcontrib><creatorcontrib>Xiao, Xingning</creatorcontrib><title>Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing</title><title>CYTA: journal of food</title><description>This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p < .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p < .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection altered the composition of bacterial communities.</description><subject>16S rDNA amplicon sequencing</subject><subject>bacterial contamination</subject><subject>Community composition</subject><subject>Composition</subject><subject>Contamination</subject><subject>Cutlery</subject><subject>Decontamination</subject><subject>Discriminant analysis</subject><subject>Disinfectants</subject><subject>Disinfection</subject><subject>Effectiveness</subject><subject>Food contamination</subject><subject>Hooks</subject><subject>Knives</subject><subject>Meat</subject><subject>Microbial contamination</subject><subject>Microbiomes</subject><subject>Microorganisms</subject><subject>NaClO disinfection</subject><subject>Pork</subject><subject>rRNA 16S</subject><subject>Sodium hypochlorite</subject><subject>Species richness</subject><subject>Transport vehicles</subject><subject>wholesaler</subject><issn>1947-6337</issn><issn>1947-6345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNp9UU1v1DAQjRBIlMJPQLLgvFt_J7lRFWgrVSC1cLYcZ7zrbWIvttNq7_zwOpvSIyePnt-8NzOvqj4SvCa4wWek5bVkrF5TTPmaMkFaLF9VJzO-koyL1y81q99W71LaYSxFTeVJ9fcWHkAPzm_Q6EwMndMDMmEcJ-_yYa72Ibnsgkfa96h3yXkL5giAtc5oc0DwoIdJHzHn0eM2DJD0AGgf4j0adbyHnFDexjBttojIOxS__jhHCf5M4E2xfl-9sXpI8OH5Pa1-f__26-JqdfPz8vri_GZlOJV5RXEnmW4aCVwA7SkWHZOS950oPxqo7HjbYEa61mIDnGNjgMiuIQJwTYCy0-p60e2D3ql9dGW2gwraqSMQ4kbpmJ0ZQLXF0UqqaQuYC0xbazsDljFCa2E6W7Q-LVohZaeScRnM1gTvy3FUyYAKWhfS54W0j6Esm7LahSn6sqNihJfZJZWksMTCKvdPKYJ9GY1gNSes_iWs5oTVc8Kl78vSVyIJcdSPIQ69yvowhGijLqedbf4r8QSw-q38</recordid><startdate>20241231</startdate><enddate>20241231</enddate><creator>He, Miao</creator><creator>Ma, Lingyan</creator><creator>Lv, Wentao</creator><creator>Xiao, Yingping</creator><creator>Yang, Hua</creator><creator>Wang, Xiaofan</creator><creator>Zhao, Jiangchao</creator><creator>Lan, Hangzhen</creator><creator>Wang, Wen</creator><creator>Xiao, Xingning</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Informa UK Limited</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7T7</scope><scope>7U7</scope><scope>7X2</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M2O</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>OTOTI</scope><scope>DOA</scope></search><sort><creationdate>20241231</creationdate><title>Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing</title><author>He, Miao ; Ma, Lingyan ; Lv, Wentao ; Xiao, Yingping ; Yang, Hua ; Wang, Xiaofan ; Zhao, Jiangchao ; Lan, Hangzhen ; Wang, Wen ; Xiao, Xingning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-20b63a886e45e2d205b3664db520bae26b498031b9f0ce440cce16b815e071e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>16S rDNA amplicon sequencing</topic><topic>bacterial contamination</topic><topic>Community composition</topic><topic>Composition</topic><topic>Contamination</topic><topic>Cutlery</topic><topic>Decontamination</topic><topic>Discriminant analysis</topic><topic>Disinfectants</topic><topic>Disinfection</topic><topic>Effectiveness</topic><topic>Food contamination</topic><topic>Hooks</topic><topic>Knives</topic><topic>Meat</topic><topic>Microbial contamination</topic><topic>Microbiomes</topic><topic>Microorganisms</topic><topic>NaClO disinfection</topic><topic>Pork</topic><topic>rRNA 16S</topic><topic>Sodium hypochlorite</topic><topic>Species richness</topic><topic>Transport vehicles</topic><topic>wholesaler</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Miao</creatorcontrib><creatorcontrib>Ma, Lingyan</creatorcontrib><creatorcontrib>Lv, Wentao</creatorcontrib><creatorcontrib>Xiao, Yingping</creatorcontrib><creatorcontrib>Yang, Hua</creatorcontrib><creatorcontrib>Wang, Xiaofan</creatorcontrib><creatorcontrib>Zhao, Jiangchao</creatorcontrib><creatorcontrib>Lan, Hangzhen</creatorcontrib><creatorcontrib>Wang, Wen</creatorcontrib><creatorcontrib>Xiao, Xingning</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Research Library</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>OSTI.GOV</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>CYTA: journal of food</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Miao</au><au>Ma, Lingyan</au><au>Lv, Wentao</au><au>Xiao, Yingping</au><au>Yang, Hua</au><au>Wang, Xiaofan</au><au>Zhao, Jiangchao</au><au>Lan, Hangzhen</au><au>Wang, Wen</au><au>Xiao, Xingning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing</atitle><jtitle>CYTA: journal of food</jtitle><date>2024-12-31</date><risdate>2024</risdate><volume>22</volume><issue>1</issue><issn>1947-6337</issn><eissn>1947-6345</eissn><abstract>This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p < .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p < .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection altered the composition of bacterial communities.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/19476337.2024.2351906</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 16S rDNA amplicon sequencing bacterial contamination Community composition Composition Contamination Cutlery Decontamination Discriminant analysis Disinfectants Disinfection Effectiveness Food contamination Hooks Knives Meat Microbial contamination Microbiomes Microorganisms NaClO disinfection Pork rRNA 16S Sodium hypochlorite Species richness Transport vehicles wholesaler |
| title | Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing |
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