Development and characterization of new microsatellite markers in Panax ginseng (C.A. Meyer) from BAC end sequences
Panax ginseng, commonly known as Korean ginseng, is a valued source of herbal medicine in Korea and China. We have developed and characterized 35 microsatellite markers in P. ginseng from available BAC end sequences. Characterization of these 35 SSR primer pairs in 14 cultivars of P. ginseng showed...
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| Veröffentlicht in: | Conservation genetics 2010-06, Vol.11 (3), p.1223-1225 |
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| container_title | Conservation genetics |
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| creator | Van Dan, Nguyen Ramchiary, Nirala Choi, Su Ryun Uhm, Tae Sik Yang, Tae-Jin Ahn, In-Ok Lim, Yong Pyo |
| description | Panax ginseng, commonly known as Korean ginseng, is a valued source of herbal medicine in Korea and China. We have developed and characterized 35 microsatellite markers in P. ginseng from available BAC end sequences. Characterization of these 35 SSR primer pairs in 14 cultivars of P. ginseng showed 12 primer pairs to be polymorphic and 19 primer pairs to be monomorphic, while the remaining four primer pairs did not produce any product. The number of alleles amplified ranged from 4 to 8 per primer pair, with an average of six alleles per locus. The expected and observed heterozygosities ranged from 0.7500 to 0.9678 and 0.5645 to 0.7109, respectively. None of these loci deviated from Hardy-Weinberg equilibrium. All of the functional primer pairs of P. ginseng showed cross-species transferability with Panax quinquefolium. The cross-species transferable markers could be used for ginseng cultivar identification, for genomic studies, including mapping of specific trait QTL/genes, and for measuring conservation of ginseng.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s10592-009-9924-y |
| format | Article |
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Meyer) from BAC end sequences</title><source>SpringerLink Journals - AutoHoldings</source><creator>Van Dan, Nguyen ; Ramchiary, Nirala ; Choi, Su Ryun ; Uhm, Tae Sik ; Yang, Tae-Jin ; Ahn, In-Ok ; Lim, Yong Pyo</creator><creatorcontrib>Van Dan, Nguyen ; Ramchiary, Nirala ; Choi, Su Ryun ; Uhm, Tae Sik ; Yang, Tae-Jin ; Ahn, In-Ok ; Lim, Yong Pyo</creatorcontrib><description>Panax ginseng, commonly known as Korean ginseng, is a valued source of herbal medicine in Korea and China. We have developed and characterized 35 microsatellite markers in P. ginseng from available BAC end sequences. Characterization of these 35 SSR primer pairs in 14 cultivars of P. ginseng showed 12 primer pairs to be polymorphic and 19 primer pairs to be monomorphic, while the remaining four primer pairs did not produce any product. The number of alleles amplified ranged from 4 to 8 per primer pair, with an average of six alleles per locus. The expected and observed heterozygosities ranged from 0.7500 to 0.9678 and 0.5645 to 0.7109, respectively. None of these loci deviated from Hardy-Weinberg equilibrium. All of the functional primer pairs of P. ginseng showed cross-species transferability with Panax quinquefolium. 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| subjects | Conservation biology Cultivars Flowers & plants Genetic markers Genomics Herbal medicine Plant populations Population genetics |
| title | Development and characterization of new microsatellite markers in Panax ginseng (C.A. Meyer) from BAC end sequences |
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