Cloning and overexpression of the DREB30 gene enhances drought and osmotic stress tolerance in transgenic potato
Potato is an extremely important food crop due to its high nutritional and economic value; however, this crop suffers losses due to water-deficient conditions. The aim of the study was to analyze the expression profiles of the StDREB30 gene and to study the enzymatic antioxidant system of transgenic...
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| description | Potato is an extremely important food crop due to its high nutritional and economic value; however, this crop suffers losses due to water-deficient conditions. The aim of the study was to analyze the expression profiles of the StDREB30 gene and to study the enzymatic antioxidant system of transgenic potatoes under water-deficient situations. The results depicted that following drought and 20% polyethylene glycol 6000 stresses, transgenic plants showed vigorous growth, increased antioxidant activities, low malondialdehyde, and enhanced differential expression of StDREB30 in transgenics compared to control plants. Results suggest that StDREB30 overexpression mitigated the negative effects of water-deficit treatments by reducing oxidative damage in plants. Hence, the StDREB30 gene retains extensive potential for crop bioengineering to generate highly tolerant crop plants.
Transgenic potatoes overexpressing StDREB30 showed enhanced tolerance to water-deficient conditions.
StDREB30 expression was almost 30, 5, and 3 folds higher in transgenic roots, shoots, and leaves respectively compared to non-transgenic plants after drought stress.
StDREB30 expression was 10, 4, and 3 folds higher in transgenic roots, shoots, and leaves, respectively after 20% PEG stress.
Transgenic plants showed vigorous growth, high antioxidant activities, and low lipid peroxidation activities in drought conditions.
The StDREB30 gene is a promising candidate for developing elite cultivars of crop plants. |
| doi_str_mv | 10.1080/17429145.2024.2364656 |
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Transgenic potatoes overexpressing StDREB30 showed enhanced tolerance to water-deficient conditions.
StDREB30 expression was almost 30, 5, and 3 folds higher in transgenic roots, shoots, and leaves respectively compared to non-transgenic plants after drought stress.
StDREB30 expression was 10, 4, and 3 folds higher in transgenic roots, shoots, and leaves, respectively after 20% PEG stress.
Transgenic plants showed vigorous growth, high antioxidant activities, and low lipid peroxidation activities in drought conditions.
The StDREB30 gene is a promising candidate for developing elite cultivars of crop plants.</description><identifier>ISSN: 1742-9145</identifier><identifier>EISSN: 1742-9153</identifier><identifier>DOI: 10.1080/17429145.2024.2364656</identifier><language>eng</language><publisher>Philadelphia: Taylor & Francis</publisher><subject>Abiotic stress ; Agricultural production ; Agronomy ; antioxidant activities ; Antioxidants ; Bioengineering ; Cloning ; Crops ; Damage tolerance ; Drought ; drought tolerance ; Enzymes ; Extreme values ; Food ; Genes ; Nutrient deficiency ; Osmotic stress ; PEG stress ; Physiology ; Polyethylene glycol ; potato transformation ; Potatoes ; Proteins ; qRT PCR ; Salinity ; Signal transduction ; StDREB30 ; Transcription factors ; Transgenic plants ; Water damage</subject><ispartof>Journal of plant interactions, 2024-12, Vol.19 (1)</ispartof><rights>2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2024</rights><rights>2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution – Non-Commercial License http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c329t-1715d9dc90e8ec00cdf590007db50ba18fa43fefe5deb37d65f87ec138e469853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/17429145.2024.2364656$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/17429145.2024.2364656$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,2102,27502,27924,27925,59143,59144</link.rule.ids></links><search><creatorcontrib>Ul Ain-Ali, Qurat</creatorcontrib><creatorcontrib>Munir, Faiza</creatorcontrib><creatorcontrib>Tahir, Muhammad</creatorcontrib><creatorcontrib>Amir, Rabia</creatorcontrib><creatorcontrib>Gul, Alvina</creatorcontrib><title>Cloning and overexpression of the DREB30 gene enhances drought and osmotic stress tolerance in transgenic potato</title><title>Journal of plant interactions</title><description>Potato is an extremely important food crop due to its high nutritional and economic value; however, this crop suffers losses due to water-deficient conditions. The aim of the study was to analyze the expression profiles of the StDREB30 gene and to study the enzymatic antioxidant system of transgenic potatoes under water-deficient situations. The results depicted that following drought and 20% polyethylene glycol 6000 stresses, transgenic plants showed vigorous growth, increased antioxidant activities, low malondialdehyde, and enhanced differential expression of StDREB30 in transgenics compared to control plants. Results suggest that StDREB30 overexpression mitigated the negative effects of water-deficit treatments by reducing oxidative damage in plants. Hence, the StDREB30 gene retains extensive potential for crop bioengineering to generate highly tolerant crop plants.
Transgenic potatoes overexpressing StDREB30 showed enhanced tolerance to water-deficient conditions.
StDREB30 expression was almost 30, 5, and 3 folds higher in transgenic roots, shoots, and leaves respectively compared to non-transgenic plants after drought stress.
StDREB30 expression was 10, 4, and 3 folds higher in transgenic roots, shoots, and leaves, respectively after 20% PEG stress.
Transgenic plants showed vigorous growth, high antioxidant activities, and low lipid peroxidation activities in drought conditions.
The StDREB30 gene is a promising candidate for developing elite cultivars of crop plants.</description><subject>Abiotic stress</subject><subject>Agricultural production</subject><subject>Agronomy</subject><subject>antioxidant activities</subject><subject>Antioxidants</subject><subject>Bioengineering</subject><subject>Cloning</subject><subject>Crops</subject><subject>Damage tolerance</subject><subject>Drought</subject><subject>drought tolerance</subject><subject>Enzymes</subject><subject>Extreme values</subject><subject>Food</subject><subject>Genes</subject><subject>Nutrient deficiency</subject><subject>Osmotic stress</subject><subject>PEG stress</subject><subject>Physiology</subject><subject>Polyethylene glycol</subject><subject>potato transformation</subject><subject>Potatoes</subject><subject>Proteins</subject><subject>qRT PCR</subject><subject>Salinity</subject><subject>Signal transduction</subject><subject>StDREB30</subject><subject>Transcription factors</subject><subject>Transgenic plants</subject><subject>Water damage</subject><issn>1742-9145</issn><issn>1742-9153</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp9kUFP3DAQhSPUSqXAT6hkqedd7NgTJzfaLRQkJCRUzpY3Hu9mlfWktrfAvydpgCMnj2be92bkVxTfBF8KXvNzoVXZCAXLkpdqWcpKVVAdFcdTf9EIkJ_eawVfiq8p7ThXpdL6uBhWPYUubJgNjtE_jPg0REypo8DIs7xF9uv-8qfkbIMBGYatDS0m5iIdNts8Y2lPuWtZyhPJMvUYJxXrAstjlUZ0HA-UbabT4rO3fcKz1_ekeLi6_LO6Xtze_b5Z_bhdtLJs8kJoAa5xbcOxxpbz1nloOOfarYGvrai9VdKjR3C4ltpV4GuNrZA1qqqpQZ4UN7OvI7szQ-z2Nj4bsp3536C4MTaOV_donGhQr6F0tQblwFvtK4eukoACvJaj1_fZa4j094Apmx0dYhjPN1JICQqqWo8qmFVtpJQi-vetgpspKPMWlJmCMq9BjdzFzHXBU9zbR4q9M9k-9xT99JHdtOZDixfBmJs0</recordid><startdate>20241231</startdate><enddate>20241231</enddate><creator>Ul Ain-Ali, Qurat</creator><creator>Munir, Faiza</creator><creator>Tahir, Muhammad</creator><creator>Amir, Rabia</creator><creator>Gul, Alvina</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20241231</creationdate><title>Cloning and overexpression of the DREB30 gene enhances drought and osmotic stress tolerance in transgenic potato</title><author>Ul Ain-Ali, Qurat ; Munir, Faiza ; Tahir, Muhammad ; Amir, Rabia ; Gul, Alvina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-1715d9dc90e8ec00cdf590007db50ba18fa43fefe5deb37d65f87ec138e469853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Abiotic stress</topic><topic>Agricultural production</topic><topic>Agronomy</topic><topic>antioxidant activities</topic><topic>Antioxidants</topic><topic>Bioengineering</topic><topic>Cloning</topic><topic>Crops</topic><topic>Damage tolerance</topic><topic>Drought</topic><topic>drought tolerance</topic><topic>Enzymes</topic><topic>Extreme values</topic><topic>Food</topic><topic>Genes</topic><topic>Nutrient deficiency</topic><topic>Osmotic stress</topic><topic>PEG stress</topic><topic>Physiology</topic><topic>Polyethylene glycol</topic><topic>potato transformation</topic><topic>Potatoes</topic><topic>Proteins</topic><topic>qRT PCR</topic><topic>Salinity</topic><topic>Signal transduction</topic><topic>StDREB30</topic><topic>Transcription factors</topic><topic>Transgenic plants</topic><topic>Water damage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ul Ain-Ali, Qurat</creatorcontrib><creatorcontrib>Munir, Faiza</creatorcontrib><creatorcontrib>Tahir, Muhammad</creatorcontrib><creatorcontrib>Amir, Rabia</creatorcontrib><creatorcontrib>Gul, Alvina</creatorcontrib><collection>Access via Taylor & Francis (Open Access Collection)</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of plant interactions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ul Ain-Ali, Qurat</au><au>Munir, Faiza</au><au>Tahir, Muhammad</au><au>Amir, Rabia</au><au>Gul, Alvina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and overexpression of the DREB30 gene enhances drought and osmotic stress tolerance in transgenic potato</atitle><jtitle>Journal of plant interactions</jtitle><date>2024-12-31</date><risdate>2024</risdate><volume>19</volume><issue>1</issue><issn>1742-9145</issn><eissn>1742-9153</eissn><abstract>Potato is an extremely important food crop due to its high nutritional and economic value; however, this crop suffers losses due to water-deficient conditions. The aim of the study was to analyze the expression profiles of the StDREB30 gene and to study the enzymatic antioxidant system of transgenic potatoes under water-deficient situations. The results depicted that following drought and 20% polyethylene glycol 6000 stresses, transgenic plants showed vigorous growth, increased antioxidant activities, low malondialdehyde, and enhanced differential expression of StDREB30 in transgenics compared to control plants. Results suggest that StDREB30 overexpression mitigated the negative effects of water-deficit treatments by reducing oxidative damage in plants. Hence, the StDREB30 gene retains extensive potential for crop bioengineering to generate highly tolerant crop plants.
Transgenic potatoes overexpressing StDREB30 showed enhanced tolerance to water-deficient conditions.
StDREB30 expression was almost 30, 5, and 3 folds higher in transgenic roots, shoots, and leaves respectively compared to non-transgenic plants after drought stress.
StDREB30 expression was 10, 4, and 3 folds higher in transgenic roots, shoots, and leaves, respectively after 20% PEG stress.
Transgenic plants showed vigorous growth, high antioxidant activities, and low lipid peroxidation activities in drought conditions.
The StDREB30 gene is a promising candidate for developing elite cultivars of crop plants.</abstract><cop>Philadelphia</cop><pub>Taylor & Francis</pub><doi>10.1080/17429145.2024.2364656</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Abiotic stress Agricultural production Agronomy antioxidant activities Antioxidants Bioengineering Cloning Crops Damage tolerance Drought drought tolerance Enzymes Extreme values Food Genes Nutrient deficiency Osmotic stress PEG stress Physiology Polyethylene glycol potato transformation Potatoes Proteins qRT PCR Salinity Signal transduction StDREB30 Transcription factors Transgenic plants Water damage |
| title | Cloning and overexpression of the DREB30 gene enhances drought and osmotic stress tolerance in transgenic potato |
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