Identifikation peroxisomaler Proteine durch quantitative SWATH-MS
Organelle proteomics is a valuable approach to identify novel proteins in a confined cellular compartment thereby increasing our understanding of the function of individual organelles in cellular metabolism or signaling. A major obstacle in annotating organelle proteomes by mass spectrometry(MS)-bas...
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| Veröffentlicht in: | Biospektrum 2024-11, Vol.30 (7), p.772-775 |
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| container_title | Biospektrum |
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| creator | Drews, Oliver Singin, Öznur Islinger, Markus |
| description | Organelle proteomics is a valuable approach to identify novel proteins in a confined cellular compartment thereby increasing our understanding of the function of individual organelles in cellular metabolism or signaling. A major obstacle in annotating organelle proteomes by mass spectrometry(MS)-based proteomics is to distinguish between true organelle constituents and contaminants co-purified during fractionation. Here we give a short summary on a recently developed quantitative SWATH-MS approach to characterize the proteome of peroxisomes from mouse liver. |
| doi_str_mv | 10.1007/s12268-024-2338-z |
| format | Article |
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| issn | 0947-0867 1868-6249 |
| language | ger |
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| source | SpringerNature Journals |
| subjects | Biochemistry Biomedical and Life Sciences Contaminants Human Genetics Life Sciences Mass spectroscopy Microbiology Organelles Pharmacology/Toxicology Proteomes Proteomics Wissenschaft Special |
| title | Identifikation peroxisomaler Proteine durch quantitative SWATH-MS |
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