Nociceptin Induces Rec8 Phosphorylation and Meiosis in Postnatal Murine Testes
Phosphorylated Rec8, a key component of cohesin, mediates the association and disassociation, “dynamics,” of chromosomes occurring in synaptonemal complex formation, crossover recombination, and sister chromatid cohesion during meiosis. Yet, the extrinsic factors triggering meiotic chromosome dynami...
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| Veröffentlicht in: | Endocrinology (Philadelphia) 2013-08, Vol.154 (8), p.2891-2899 |
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| description | Phosphorylated Rec8, a key component of cohesin, mediates the association and disassociation, “dynamics,” of chromosomes occurring in synaptonemal complex formation, crossover recombination, and sister chromatid cohesion during meiosis. Yet, the extrinsic factors triggering meiotic chromosome dynamics remain elusive. We have recently found that nociceptin, known as a neuropeptide, is up-regulated by follicle-stimulating hormone in Sertoli cells in postnatal murine testes; however, very little is known about the functional role of nociceptin in spermatogenesis. Here, we show that nociceptin induces Rec8 phosphorylation, triggering chromosome dynamics, in spermatocytes during meiosis in postnatal murine testes. The nociceptin receptor Oprl-1 is exclusively expressed in the plasma membrane of testicular germ cells, mostly spermatocytes. Treatment of testes with nociceptin resulted in a rapid phosphorylation of Rec8. Injection of nociceptin into mice stimulated Rec8 phosphorylation and meiotic chromosome dynamics in testes, whereas injection of nocistatin, a specific inhibitor of nociceptin, abolished them. These findings suggest that nociceptin is a novel extrinsic factor that plays a crucial role in the progress of meiosis. |
| doi_str_mv | 10.1210/en.2012-1977 |
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Yet, the extrinsic factors triggering meiotic chromosome dynamics remain elusive. We have recently found that nociceptin, known as a neuropeptide, is up-regulated by follicle-stimulating hormone in Sertoli cells in postnatal murine testes; however, very little is known about the functional role of nociceptin in spermatogenesis. Here, we show that nociceptin induces Rec8 phosphorylation, triggering chromosome dynamics, in spermatocytes during meiosis in postnatal murine testes. The nociceptin receptor Oprl-1 is exclusively expressed in the plasma membrane of testicular germ cells, mostly spermatocytes. Treatment of testes with nociceptin resulted in a rapid phosphorylation of Rec8. Injection of nociceptin into mice stimulated Rec8 phosphorylation and meiotic chromosome dynamics in testes, whereas injection of nocistatin, a specific inhibitor of nociceptin, abolished them. These findings suggest that nociceptin is a novel extrinsic factor that plays a crucial role in the progress of meiosis.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2012-1977</identifier><identifier>PMID: 23720425</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Chevy Chase, MD: Endocrine Society</publisher><subject>Analgesics, Opioid - pharmacology ; Animals ; Biological and medical sciences ; Blotting, Western ; Cell Cycle Proteins ; Cell division ; Cell Membrane - metabolism ; Chromosome Pairing - drug effects ; Chromosomes ; Cohesin ; Complex formation ; Dynamics ; Follicle-stimulating hormone ; Fundamental and applied biological sciences. Psychology ; Gametocytes ; Germ cells ; Immunohistochemistry ; Injection ; Male ; Meiosis ; Meiosis - drug effects ; Mice ; Mice, Inbred C57BL ; Nociceptin ; Nociceptin Receptor ; Nuclear Proteins - metabolism ; Opioid Peptides - pharmacology ; Phosphoproteins - metabolism ; Phosphorylation ; Phosphorylation - drug effects ; Postpartum period ; Receptors, Opioid - metabolism ; Sertoli cells ; Sertoli Cells - drug effects ; Sertoli Cells - metabolism ; Spermatocytes ; Spermatocytes - metabolism ; Spermatogenesis ; Synaptonemal complex ; Testes ; Testis - drug effects ; Testis - metabolism ; Vasodilator Agents - pharmacology ; Vertebrates: endocrinology</subject><ispartof>Endocrinology (Philadelphia), 2013-08, Vol.154 (8), p.2891-2899</ispartof><rights>Copyright © 2013 by The Endocrine Society</rights><rights>Copyright © 2013 by The Endocrine Society 2013</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-45626f9768ff98131018ff5bc19a50e839265ad8bce814f157e74b59f44260113</citedby><cites>FETCH-LOGICAL-c554t-45626f9768ff98131018ff5bc19a50e839265ad8bce814f157e74b59f44260113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27574374$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23720425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eto, Ko</creatorcontrib><creatorcontrib>Shiotsuki, Masahiro</creatorcontrib><creatorcontrib>Abe, Shin-ichi</creatorcontrib><title>Nociceptin Induces Rec8 Phosphorylation and Meiosis in Postnatal Murine Testes</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Phosphorylated Rec8, a key component of cohesin, mediates the association and disassociation, “dynamics,” of chromosomes occurring in synaptonemal complex formation, crossover recombination, and sister chromatid cohesion during meiosis. Yet, the extrinsic factors triggering meiotic chromosome dynamics remain elusive. We have recently found that nociceptin, known as a neuropeptide, is up-regulated by follicle-stimulating hormone in Sertoli cells in postnatal murine testes; however, very little is known about the functional role of nociceptin in spermatogenesis. Here, we show that nociceptin induces Rec8 phosphorylation, triggering chromosome dynamics, in spermatocytes during meiosis in postnatal murine testes. The nociceptin receptor Oprl-1 is exclusively expressed in the plasma membrane of testicular germ cells, mostly spermatocytes. Treatment of testes with nociceptin resulted in a rapid phosphorylation of Rec8. Injection of nociceptin into mice stimulated Rec8 phosphorylation and meiotic chromosome dynamics in testes, whereas injection of nocistatin, a specific inhibitor of nociceptin, abolished them. These findings suggest that nociceptin is a novel extrinsic factor that plays a crucial role in the progress of meiosis.</description><subject>Analgesics, Opioid - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Cycle Proteins</subject><subject>Cell division</subject><subject>Cell Membrane - metabolism</subject><subject>Chromosome Pairing - drug effects</subject><subject>Chromosomes</subject><subject>Cohesin</subject><subject>Complex formation</subject><subject>Dynamics</subject><subject>Follicle-stimulating hormone</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gametocytes</subject><subject>Germ cells</subject><subject>Immunohistochemistry</subject><subject>Injection</subject><subject>Male</subject><subject>Meiosis</subject><subject>Meiosis - drug effects</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Nociceptin</subject><subject>Nociceptin Receptor</subject><subject>Nuclear Proteins - metabolism</subject><subject>Opioid Peptides - pharmacology</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorylation</subject><subject>Phosphorylation - drug effects</subject><subject>Postpartum period</subject><subject>Receptors, Opioid - metabolism</subject><subject>Sertoli cells</subject><subject>Sertoli Cells - drug effects</subject><subject>Sertoli Cells - metabolism</subject><subject>Spermatocytes</subject><subject>Spermatocytes - metabolism</subject><subject>Spermatogenesis</subject><subject>Synaptonemal complex</subject><subject>Testes</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Vasodilator Agents - pharmacology</subject><subject>Vertebrates: endocrinology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtLAzEURoMotlZ3rmVAxI1Tc_OYzCxFfIGPInU9pJk7dEqbjMnMwn9vSqvdKASSwOH77j2EnAIdAwN6jXbMKLAUCqX2yBAKIVMFiu6TIaXAU8WYGpCjEBbxK4Tgh2TAuGJUMDkkr6_ONAbbrrHJk616gyF5R5Mnk7kL7dz5r6XuGmcTbavkBRsXmpBEduJCZ3Wnl8lL7xuLyRRDh-GYHNR6GfBke4_Ix_3d9PYxfX57eLq9eU6NlKJLhcxYVhcqy-u6yIEDhfiSMwOFlhRzXrBM6iqfGcxB1CAVKjGTRS0EyygAH5HzTW7r3Wcfq8uF672NlSUHTjMWBfBIXW0o410IHuuy9c1K-68SaLmWV6It1_LKtbyIn21D-9kKq1_4x1YELraADkYva6-tacKOU1IJHs-IXG4417f_VabbSr4h0VbOrEW2HkPYbfPnoN9dhZIW</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Eto, Ko</creator><creator>Shiotsuki, Masahiro</creator><creator>Abe, Shin-ichi</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20130801</creationdate><title>Nociceptin Induces Rec8 Phosphorylation and Meiosis in Postnatal Murine Testes</title><author>Eto, Ko ; Shiotsuki, Masahiro ; Abe, Shin-ichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-45626f9768ff98131018ff5bc19a50e839265ad8bce814f157e74b59f44260113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analgesics, Opioid - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Cycle Proteins</topic><topic>Cell division</topic><topic>Cell Membrane - metabolism</topic><topic>Chromosome Pairing - drug effects</topic><topic>Chromosomes</topic><topic>Cohesin</topic><topic>Complex formation</topic><topic>Dynamics</topic><topic>Follicle-stimulating hormone</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gametocytes</topic><topic>Germ cells</topic><topic>Immunohistochemistry</topic><topic>Injection</topic><topic>Male</topic><topic>Meiosis</topic><topic>Meiosis - drug effects</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Nociceptin</topic><topic>Nociceptin Receptor</topic><topic>Nuclear Proteins - metabolism</topic><topic>Opioid Peptides - pharmacology</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorylation</topic><topic>Phosphorylation - drug effects</topic><topic>Postpartum period</topic><topic>Receptors, Opioid - metabolism</topic><topic>Sertoli cells</topic><topic>Sertoli Cells - drug effects</topic><topic>Sertoli Cells - metabolism</topic><topic>Spermatocytes</topic><topic>Spermatocytes - metabolism</topic><topic>Spermatogenesis</topic><topic>Synaptonemal complex</topic><topic>Testes</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Vasodilator Agents - pharmacology</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eto, Ko</creatorcontrib><creatorcontrib>Shiotsuki, Masahiro</creatorcontrib><creatorcontrib>Abe, Shin-ichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eto, Ko</au><au>Shiotsuki, Masahiro</au><au>Abe, Shin-ichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nociceptin Induces Rec8 Phosphorylation and Meiosis in Postnatal Murine Testes</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>154</volume><issue>8</issue><spage>2891</spage><epage>2899</epage><pages>2891-2899</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>Phosphorylated Rec8, a key component of cohesin, mediates the association and disassociation, “dynamics,” of chromosomes occurring in synaptonemal complex formation, crossover recombination, and sister chromatid cohesion during meiosis. Yet, the extrinsic factors triggering meiotic chromosome dynamics remain elusive. We have recently found that nociceptin, known as a neuropeptide, is up-regulated by follicle-stimulating hormone in Sertoli cells in postnatal murine testes; however, very little is known about the functional role of nociceptin in spermatogenesis. Here, we show that nociceptin induces Rec8 phosphorylation, triggering chromosome dynamics, in spermatocytes during meiosis in postnatal murine testes. The nociceptin receptor Oprl-1 is exclusively expressed in the plasma membrane of testicular germ cells, mostly spermatocytes. Treatment of testes with nociceptin resulted in a rapid phosphorylation of Rec8. Injection of nociceptin into mice stimulated Rec8 phosphorylation and meiotic chromosome dynamics in testes, whereas injection of nocistatin, a specific inhibitor of nociceptin, abolished them. These findings suggest that nociceptin is a novel extrinsic factor that plays a crucial role in the progress of meiosis.</abstract><cop>Chevy Chase, MD</cop><pub>Endocrine Society</pub><pmid>23720425</pmid><doi>10.1210/en.2012-1977</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Journals@Ovid Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analgesics, Opioid - pharmacology Animals Biological and medical sciences Blotting, Western Cell Cycle Proteins Cell division Cell Membrane - metabolism Chromosome Pairing - drug effects Chromosomes Cohesin Complex formation Dynamics Follicle-stimulating hormone Fundamental and applied biological sciences. Psychology Gametocytes Germ cells Immunohistochemistry Injection Male Meiosis Meiosis - drug effects Mice Mice, Inbred C57BL Nociceptin Nociceptin Receptor Nuclear Proteins - metabolism Opioid Peptides - pharmacology Phosphoproteins - metabolism Phosphorylation Phosphorylation - drug effects Postpartum period Receptors, Opioid - metabolism Sertoli cells Sertoli Cells - drug effects Sertoli Cells - metabolism Spermatocytes Spermatocytes - metabolism Spermatogenesis Synaptonemal complex Testes Testis - drug effects Testis - metabolism Vasodilator Agents - pharmacology Vertebrates: endocrinology |
| title | Nociceptin Induces Rec8 Phosphorylation and Meiosis in Postnatal Murine Testes |
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