A Secreted Fluorescent Reporter Targeted to Pituitary Growth Hormone Cells in Transgenic Mice

In stable transfection experiments in the GH-producing GC cell line, a construct containing the entire signal peptide and the first 22 residues of human GH linked in frame with enhanced green fluorescent protein (eGFP), produced brightly fluorescent cells with a granular distribution of eGFP. This e...

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Veröffentlicht in:	Endocrinology (Philadelphia) 2000-12, Vol.141 (12), p.4681-4689
Hauptverfasser:	Magoulas, C, McGuinness, L, Balthasar, N, Carmignac, D. F, Sesay, A. K, Mathers, K. E, Christian, H, Candeil, L, Bonnefont, X, Mollard, P, Robinson, I. C. A. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcium (intracellular) Calcium ions Clusters Electron microscopy Flow cytometry Fluorescence Green fluorescent protein Growth hormones Microscopy Pituitary Scanning microscopy Secretory vesicles Transfection Transgenes Transgenic animals Transgenic mice
Online-Zugang:	Volltext
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