Decidual prolactin-related protein: heterologous expression and characterization

As a first step in understanding the role of decidual PRL-related protein (dPRP) during pregnancy, we have generated recombinant dPRP protein. In this report, we present data on the generation, purification, and characterization of recombinant dPRP protein. The dPRP complementary DNA was subcloned i...

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Veröffentlicht in:	Endocrinology (Philadelphia) 1996-12, Vol.137 (12), p.5558-5566
Hauptverfasser:	Rasmussen, C A, Hashizume, K, Orwig, K E, Xu, L, Soares, M J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid sequence Amino acids Animals CAP37 protein Cell proliferation Cell size CHO Cells Cricetinae Decidua Drug Interactions Electrophoretic mobility Electroporation Extracellular matrix Heparin Heparin - pharmacology Immunoreactivity Liquid chromatography Lymphoma Mice Mice, Nude Neovascularization, Pathologic - pathology Neovascularization, Physiologic - drug effects Polyclonal antibodies Pregnancy Prolactin Prolactin - analogs & derivatives Prolactin - isolation & purification Prolactin - metabolism Prolactin - pharmacology Protein purification Proteins Rats Rats, Inbred Strains Recombinant Proteins Sheep Size exclusion chromatography Ultrafiltration
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