Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae)
One of the challenges in the management of Kinosternon scorpioides is related to egg quality, since mineral deficiencies affect egg production. The aim of this study was to evaluate the ultrastructure and mineral composition of eggshells, and their relation to K. scorpioides embryonic skeletogenesis...
Gespeichert in:
| Veröffentlicht in: | Zoomorphology 2024-12, Vol.143 (3), p.757-766 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 766 |
|---|---|
| container_issue | 3 |
| container_start_page | 757 |
| container_title | Zoomorphology |
| container_volume | 143 |
| creator | Leal, Cassiane Braga, Brenda Cardoso, Deise Ferreira, Ana Cássia Oliveira-Bahia, Verônica Marques, José Ribamar Guimarães, Diva Anelie |
| description | One of the challenges in the management of
Kinosternon scorpioides
is related to egg quality, since mineral deficiencies affect egg production. The aim of this study was to evaluate the ultrastructure and mineral composition of eggshells, and their relation to
K. scorpioides
embryonic skeletogenesis. Eggs were collected, incubated and distributed into three groups: 1- pre-ossification (PO); 2- bone ossification (BO); and 3- complete ossification (CO). Nine samples were included in the PO and BO groups, and six in the CO group, for a total of 24 eggs. The morphological and mineral analyses of the eggs were performed by scanning electron microscopy coupled with energy-dispersive spectroscopy. The embryos from the BO and CO groups were cleared with potassium hydroxide and stained with alcian blue and alizarin red to observe osteogenesis. The results showed a significant difference (
p
|
| doi_str_mv | 10.1007/s00435-024-00676-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_3121480004</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3121480004</sourcerecordid><originalsourceid>FETCH-LOGICAL-c200t-d6315d48c89331707b3983a4fadb4613283834ddc66383513732cfd91e75bc2a3</originalsourceid><addsrcrecordid>eNp9kD9PwzAQxS0EEqXwBZgsscAQOPvcJGVDqPwRSCwgsVmu7RRXaRx8ycDCZyehSN2Y7nR6793Tj7FTAZcCoLgiAIWzDKTKAPIiz8QemwiFMpOo3vfZBAoJmRT4fsiOiNYAQuUKJ-x7sVrRh69r3tddMtSl3nZ98tw0jm9C45OpuY2bNlLoQmy461NoVrz78DxS5-PKN54C8Vjxp9CMp9QMMrIxtSEG54mfv3rqejeE0fVOFJzxF8fsoDI1-ZO_OWVvd4vX24fs-eX-8fbmObMSoMtcjmLmVGnLOaIooFjivESjKuOWKhcoSyxROWfzfFhmAguUtnJz4YvZ0kqDU3a2zW1T_OyHOnod-9QMLzUKKVQJI8Apk1uVTZEo-Uq3KWxM-tIC9MhZbznrgbP-5azFYMKtidqRjE-76H9cP3cTgr0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3121480004</pqid></control><display><type>article</type><title>Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae)</title><source>SpringerNature Journals</source><creator>Leal, Cassiane ; Braga, Brenda ; Cardoso, Deise ; Ferreira, Ana Cássia ; Oliveira-Bahia, Verônica ; Marques, José Ribamar ; Guimarães, Diva Anelie</creator><creatorcontrib>Leal, Cassiane ; Braga, Brenda ; Cardoso, Deise ; Ferreira, Ana Cássia ; Oliveira-Bahia, Verônica ; Marques, José Ribamar ; Guimarães, Diva Anelie</creatorcontrib><description>One of the challenges in the management of
Kinosternon scorpioides
is related to egg quality, since mineral deficiencies affect egg production. The aim of this study was to evaluate the ultrastructure and mineral composition of eggshells, and their relation to
K. scorpioides
embryonic skeletogenesis. Eggs were collected, incubated and distributed into three groups: 1- pre-ossification (PO); 2- bone ossification (BO); and 3- complete ossification (CO). Nine samples were included in the PO and BO groups, and six in the CO group, for a total of 24 eggs. The morphological and mineral analyses of the eggs were performed by scanning electron microscopy coupled with energy-dispersive spectroscopy. The embryos from the BO and CO groups were cleared with potassium hydroxide and stained with alcian blue and alizarin red to observe osteogenesis. The results showed a significant difference (
p
< 0.05) in the amount of shell calcium between the PO (63.14%) and CO (41.95%) groups, as well as in the shell thickness between the same groups (PO: 305.38 ± 60.27 μm; CO: 197.67 ± 40.28 μm), and BO (289.56 ± 55.87 μm) and CO groups. It is suggested that the gradual mobilization of calcium by the embryo is reflected in the decrease in shell thickness. In addition, the first structures to ossify were the dermatocranium and mandible. The CO group, in which the individuals all had established bone structures, had the lowest calcium concentration of eggshells. Approximately 21% of the shell calcium was absorbed, which suggests participation in the embryo skeletogenesis. Understanding calcium dynamics as they relate to shell quality and embryonic development of
K. scorpioides
highlights the importance of including this mineral in the diet of breeding females and, consequently, in improving offspring production.</description><identifier>ISSN: 0720-213X</identifier><identifier>EISSN: 1432-234X</identifier><identifier>DOI: 10.1007/s00435-024-00676-1</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animal Anatomy ; Animal Systematics/Taxonomy/Biogeography ; Biomedical and Life Sciences ; Bone composition ; Calcium ; Calcium mobilization ; Calcium signalling ; Developmental Biology ; Egg production ; Egg shells ; Eggs ; Electron microscopy ; Embryogenesis ; Embryonic development ; Embryonic growth stage ; Embryos ; Evolutionary Biology ; Histology ; Hydroxides ; Kinosternon scorpioides ; Life Sciences ; Mineral composition ; Minerals ; Morphology ; Offspring ; Ossification ; Osteogenesis ; Potassium ; Potassium hydroxide ; Scanning electron microscopy ; Skeletogenesis ; Spectroscopy ; Ultrastructure</subject><ispartof>Zoomorphology, 2024-12, Vol.143 (3), p.757-766</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c200t-d6315d48c89331707b3983a4fadb4613283834ddc66383513732cfd91e75bc2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00435-024-00676-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00435-024-00676-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Leal, Cassiane</creatorcontrib><creatorcontrib>Braga, Brenda</creatorcontrib><creatorcontrib>Cardoso, Deise</creatorcontrib><creatorcontrib>Ferreira, Ana Cássia</creatorcontrib><creatorcontrib>Oliveira-Bahia, Verônica</creatorcontrib><creatorcontrib>Marques, José Ribamar</creatorcontrib><creatorcontrib>Guimarães, Diva Anelie</creatorcontrib><title>Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae)</title><title>Zoomorphology</title><addtitle>Zoomorphology</addtitle><description>One of the challenges in the management of
Kinosternon scorpioides
is related to egg quality, since mineral deficiencies affect egg production. The aim of this study was to evaluate the ultrastructure and mineral composition of eggshells, and their relation to
K. scorpioides
embryonic skeletogenesis. Eggs were collected, incubated and distributed into three groups: 1- pre-ossification (PO); 2- bone ossification (BO); and 3- complete ossification (CO). Nine samples were included in the PO and BO groups, and six in the CO group, for a total of 24 eggs. The morphological and mineral analyses of the eggs were performed by scanning electron microscopy coupled with energy-dispersive spectroscopy. The embryos from the BO and CO groups were cleared with potassium hydroxide and stained with alcian blue and alizarin red to observe osteogenesis. The results showed a significant difference (
p
< 0.05) in the amount of shell calcium between the PO (63.14%) and CO (41.95%) groups, as well as in the shell thickness between the same groups (PO: 305.38 ± 60.27 μm; CO: 197.67 ± 40.28 μm), and BO (289.56 ± 55.87 μm) and CO groups. It is suggested that the gradual mobilization of calcium by the embryo is reflected in the decrease in shell thickness. In addition, the first structures to ossify were the dermatocranium and mandible. The CO group, in which the individuals all had established bone structures, had the lowest calcium concentration of eggshells. Approximately 21% of the shell calcium was absorbed, which suggests participation in the embryo skeletogenesis. Understanding calcium dynamics as they relate to shell quality and embryonic development of
K. scorpioides
highlights the importance of including this mineral in the diet of breeding females and, consequently, in improving offspring production.</description><subject>Animal Anatomy</subject><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Biomedical and Life Sciences</subject><subject>Bone composition</subject><subject>Calcium</subject><subject>Calcium mobilization</subject><subject>Calcium signalling</subject><subject>Developmental Biology</subject><subject>Egg production</subject><subject>Egg shells</subject><subject>Eggs</subject><subject>Electron microscopy</subject><subject>Embryogenesis</subject><subject>Embryonic development</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Evolutionary Biology</subject><subject>Histology</subject><subject>Hydroxides</subject><subject>Kinosternon scorpioides</subject><subject>Life Sciences</subject><subject>Mineral composition</subject><subject>Minerals</subject><subject>Morphology</subject><subject>Offspring</subject><subject>Ossification</subject><subject>Osteogenesis</subject><subject>Potassium</subject><subject>Potassium hydroxide</subject><subject>Scanning electron microscopy</subject><subject>Skeletogenesis</subject><subject>Spectroscopy</subject><subject>Ultrastructure</subject><issn>0720-213X</issn><issn>1432-234X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kD9PwzAQxS0EEqXwBZgsscAQOPvcJGVDqPwRSCwgsVmu7RRXaRx8ycDCZyehSN2Y7nR6793Tj7FTAZcCoLgiAIWzDKTKAPIiz8QemwiFMpOo3vfZBAoJmRT4fsiOiNYAQuUKJ-x7sVrRh69r3tddMtSl3nZ98tw0jm9C45OpuY2bNlLoQmy461NoVrz78DxS5-PKN54C8Vjxp9CMp9QMMrIxtSEG54mfv3rqejeE0fVOFJzxF8fsoDI1-ZO_OWVvd4vX24fs-eX-8fbmObMSoMtcjmLmVGnLOaIooFjivESjKuOWKhcoSyxROWfzfFhmAguUtnJz4YvZ0kqDU3a2zW1T_OyHOnod-9QMLzUKKVQJI8Apk1uVTZEo-Uq3KWxM-tIC9MhZbznrgbP-5azFYMKtidqRjE-76H9cP3cTgr0</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Leal, Cassiane</creator><creator>Braga, Brenda</creator><creator>Cardoso, Deise</creator><creator>Ferreira, Ana Cássia</creator><creator>Oliveira-Bahia, Verônica</creator><creator>Marques, José Ribamar</creator><creator>Guimarães, Diva Anelie</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20241201</creationdate><title>Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae)</title><author>Leal, Cassiane ; Braga, Brenda ; Cardoso, Deise ; Ferreira, Ana Cássia ; Oliveira-Bahia, Verônica ; Marques, José Ribamar ; Guimarães, Diva Anelie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c200t-d6315d48c89331707b3983a4fadb4613283834ddc66383513732cfd91e75bc2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal Anatomy</topic><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Biomedical and Life Sciences</topic><topic>Bone composition</topic><topic>Calcium</topic><topic>Calcium mobilization</topic><topic>Calcium signalling</topic><topic>Developmental Biology</topic><topic>Egg production</topic><topic>Egg shells</topic><topic>Eggs</topic><topic>Electron microscopy</topic><topic>Embryogenesis</topic><topic>Embryonic development</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Evolutionary Biology</topic><topic>Histology</topic><topic>Hydroxides</topic><topic>Kinosternon scorpioides</topic><topic>Life Sciences</topic><topic>Mineral composition</topic><topic>Minerals</topic><topic>Morphology</topic><topic>Offspring</topic><topic>Ossification</topic><topic>Osteogenesis</topic><topic>Potassium</topic><topic>Potassium hydroxide</topic><topic>Scanning electron microscopy</topic><topic>Skeletogenesis</topic><topic>Spectroscopy</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leal, Cassiane</creatorcontrib><creatorcontrib>Braga, Brenda</creatorcontrib><creatorcontrib>Cardoso, Deise</creatorcontrib><creatorcontrib>Ferreira, Ana Cássia</creatorcontrib><creatorcontrib>Oliveira-Bahia, Verônica</creatorcontrib><creatorcontrib>Marques, José Ribamar</creatorcontrib><creatorcontrib>Guimarães, Diva Anelie</creatorcontrib><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Zoomorphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leal, Cassiane</au><au>Braga, Brenda</au><au>Cardoso, Deise</au><au>Ferreira, Ana Cássia</au><au>Oliveira-Bahia, Verônica</au><au>Marques, José Ribamar</au><au>Guimarães, Diva Anelie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae)</atitle><jtitle>Zoomorphology</jtitle><stitle>Zoomorphology</stitle><date>2024-12-01</date><risdate>2024</risdate><volume>143</volume><issue>3</issue><spage>757</spage><epage>766</epage><pages>757-766</pages><issn>0720-213X</issn><eissn>1432-234X</eissn><abstract>One of the challenges in the management of
Kinosternon scorpioides
is related to egg quality, since mineral deficiencies affect egg production. The aim of this study was to evaluate the ultrastructure and mineral composition of eggshells, and their relation to
K. scorpioides
embryonic skeletogenesis. Eggs were collected, incubated and distributed into three groups: 1- pre-ossification (PO); 2- bone ossification (BO); and 3- complete ossification (CO). Nine samples were included in the PO and BO groups, and six in the CO group, for a total of 24 eggs. The morphological and mineral analyses of the eggs were performed by scanning electron microscopy coupled with energy-dispersive spectroscopy. The embryos from the BO and CO groups were cleared with potassium hydroxide and stained with alcian blue and alizarin red to observe osteogenesis. The results showed a significant difference (
p
< 0.05) in the amount of shell calcium between the PO (63.14%) and CO (41.95%) groups, as well as in the shell thickness between the same groups (PO: 305.38 ± 60.27 μm; CO: 197.67 ± 40.28 μm), and BO (289.56 ± 55.87 μm) and CO groups. It is suggested that the gradual mobilization of calcium by the embryo is reflected in the decrease in shell thickness. In addition, the first structures to ossify were the dermatocranium and mandible. The CO group, in which the individuals all had established bone structures, had the lowest calcium concentration of eggshells. Approximately 21% of the shell calcium was absorbed, which suggests participation in the embryo skeletogenesis. Understanding calcium dynamics as they relate to shell quality and embryonic development of
K. scorpioides
highlights the importance of including this mineral in the diet of breeding females and, consequently, in improving offspring production.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00435-024-00676-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0720-213X |
| ispartof | Zoomorphology, 2024-12, Vol.143 (3), p.757-766 |
| issn | 0720-213X 1432-234X |
| language | eng |
| recordid | cdi_proquest_journals_3121480004 |
| source | SpringerNature Journals |
| subjects | Animal Anatomy Animal Systematics/Taxonomy/Biogeography Biomedical and Life Sciences Bone composition Calcium Calcium mobilization Calcium signalling Developmental Biology Egg production Egg shells Eggs Electron microscopy Embryogenesis Embryonic development Embryonic growth stage Embryos Evolutionary Biology Histology Hydroxides Kinosternon scorpioides Life Sciences Mineral composition Minerals Morphology Offspring Ossification Osteogenesis Potassium Potassium hydroxide Scanning electron microscopy Skeletogenesis Spectroscopy Ultrastructure |
| title | Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T22%3A23%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Eggshell%20ultrastructure%20and%20mineral%20composition%20during%20the%20osteogenesis%20of%20Kinosternon%20scorpioides%20(Testudines:%20Kinosternidae)&rft.jtitle=Zoomorphology&rft.au=Leal,%20Cassiane&rft.date=2024-12-01&rft.volume=143&rft.issue=3&rft.spage=757&rft.epage=766&rft.pages=757-766&rft.issn=0720-213X&rft.eissn=1432-234X&rft_id=info:doi/10.1007/s00435-024-00676-1&rft_dat=%3Cproquest_cross%3E3121480004%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3121480004&rft_id=info:pmid/&rfr_iscdi=true |