Site-Directed Mutagenesis of Two-Domain Laccase ScaSL for Obtaining a Biocatalyst with Improved Characteristics

Analysis of the structure of two-domain laccase ScaSL from Streptomyces carpinensis VKM Ac-1300 (with a middle-redox potential) revealed determinants that could affect the increased potential of ScaSL. Site-directed mutagenesis of the ScaSL laccase was carried out, and mutants H286A, H286T, H286W, a...

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Veröffentlicht in:	Catalysts 2024-10, Vol.14 (10), p.694
Hauptverfasser:	Trubitsina, Liubov, Trubitsin, Ivan, Gabdulkhakov, Azat, Abdullatypov, Azat, Larionova, Anna, Petrakova, Marina, Trubitsyn, Vladimir, Egorov, Konstantin, Tishchenko, Svetlana, Leontievsky, Alexey, Alferov, Sergey, Ponamoreva, Olga
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidic oxides Amino acids Analysis Copper Crystal structure Crystals Decoloring Dyes Electrode potentials Electron transfer Enzyme activity Enzymes Laccase Ligands Mutagenesis Mutation Oxidases Oxidation Proteins Structure Thermal stability
Online-Zugang:	Volltext
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