Candida parapsilosis CMGB-YT Biosurfactant for Treatment of Heavy Metal- and Microbial-Contaminated Wastewater

During the last few decades, water pollution has become a growing concern at international level. To date, only a few Candida parapsilosis strains were successfully used in environmental remediation. In the present article, the strain C. parapsilosis CMGB-YT was studied for its ability to assimilate...

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Veröffentlicht in:Processes 2024-07, Vol.12 (7), p.1471
Hauptverfasser: Csutak, Ortansa Elisabeta, Nicula, Nicoleta-Oana, Lungulescu, Eduard-Marius, Marinescu, Virgil Emanuel, Gifu, Ioana Catalina, Corbu, Viorica Maria
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container_end_page
container_issue 7
container_start_page 1471
container_title Processes
container_volume 12
creator Csutak, Ortansa Elisabeta
Nicula, Nicoleta-Oana
Lungulescu, Eduard-Marius
Marinescu, Virgil Emanuel
Gifu, Ioana Catalina
Corbu, Viorica Maria
description During the last few decades, water pollution has become a growing concern at international level. To date, only a few Candida parapsilosis strains were successfully used in environmental remediation. In the present article, the strain C. parapsilosis CMGB-YT was studied for its ability to assimilate hydrophobic substrates and to produce biosurfactants with antimicrobial activity and positive effects on heavy metal removal from contaminated wastewaters. The strain C. parapsilosis CMGB-YT was grown on yeast peptone (YP) media with 1% n-decane, n-dodecane, n-tetradecane, n-hexadecane, as well as commercial sunflower and olive oils. The production of the biosurfactant was evaluated using the emulsification index (E24%). The surface properties and emulsifying stability of the biosurfactant were determined. The effect of the biosurfactant on the cell growth of two strains of Rhodotorula mucilaginosa and on their removal capacity of lead (0.032 g/L) and cadmium (0.030 g/L) ions from synthetic wastewater were also studied. The antimicrobial potential of 20 mg/mL and 40 mg/mL biosurfactant was established in the presence of pathogenic Candida krusei strains. C. parapsilosis CMGB-YT assimilated n-hexadecane with good rates over 216 h and produced an anionic glycolipidic biosurfactant with stable E24% towards long-chain carbon compounds at different temperatures, with an alkaline pH and high salinity (10% NaCl). The biosurfactant reduced the surface tension to 53.58 ± 0.42 mN/m, while the critical micellar concentration (CMC) was reached at 4.2% biosurfactant. The crude biosurfactant (5%) enhanced R. mucilaginosa growth in heavy metal-contaminated wastewater, increased chemical oxygen demand (COD) removal of up to 80%, and improved Cd2+ removal by 10%. Additionally, the concentrated biosurfactant effectively prevented Candida krusei biofilm formation. In conclusion, the biosurfactant produced by C. parapsilosis CMGB-YT demonstrates promising potential for the efficient treatment of wastewater contaminated with heavy metals and microbial pathogens.
doi_str_mv 10.3390/pr12071471
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To date, only a few Candida parapsilosis strains were successfully used in environmental remediation. In the present article, the strain C. parapsilosis CMGB-YT was studied for its ability to assimilate hydrophobic substrates and to produce biosurfactants with antimicrobial activity and positive effects on heavy metal removal from contaminated wastewaters. The strain C. parapsilosis CMGB-YT was grown on yeast peptone (YP) media with 1% n-decane, n-dodecane, n-tetradecane, n-hexadecane, as well as commercial sunflower and olive oils. The production of the biosurfactant was evaluated using the emulsification index (E24%). The surface properties and emulsifying stability of the biosurfactant were determined. The effect of the biosurfactant on the cell growth of two strains of Rhodotorula mucilaginosa and on their removal capacity of lead (0.032 g/L) and cadmium (0.030 g/L) ions from synthetic wastewater were also studied. The antimicrobial potential of 20 mg/mL and 40 mg/mL biosurfactant was established in the presence of pathogenic Candida krusei strains. C. parapsilosis CMGB-YT assimilated n-hexadecane with good rates over 216 h and produced an anionic glycolipidic biosurfactant with stable E24% towards long-chain carbon compounds at different temperatures, with an alkaline pH and high salinity (10% NaCl). The biosurfactant reduced the surface tension to 53.58 ± 0.42 mN/m, while the critical micellar concentration (CMC) was reached at 4.2% biosurfactant. The crude biosurfactant (5%) enhanced R. mucilaginosa growth in heavy metal-contaminated wastewater, increased chemical oxygen demand (COD) removal of up to 80%, and improved Cd2+ removal by 10%. Additionally, the concentrated biosurfactant effectively prevented Candida krusei biofilm formation. 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The antimicrobial potential of 20 mg/mL and 40 mg/mL biosurfactant was established in the presence of pathogenic Candida krusei strains. C. parapsilosis CMGB-YT assimilated n-hexadecane with good rates over 216 h and produced an anionic glycolipidic biosurfactant with stable E24% towards long-chain carbon compounds at different temperatures, with an alkaline pH and high salinity (10% NaCl). The biosurfactant reduced the surface tension to 53.58 ± 0.42 mN/m, while the critical micellar concentration (CMC) was reached at 4.2% biosurfactant. The crude biosurfactant (5%) enhanced R. mucilaginosa growth in heavy metal-contaminated wastewater, increased chemical oxygen demand (COD) removal of up to 80%, and improved Cd2+ removal by 10%. Additionally, the concentrated biosurfactant effectively prevented Candida krusei biofilm formation. 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To date, only a few Candida parapsilosis strains were successfully used in environmental remediation. In the present article, the strain C. parapsilosis CMGB-YT was studied for its ability to assimilate hydrophobic substrates and to produce biosurfactants with antimicrobial activity and positive effects on heavy metal removal from contaminated wastewaters. The strain C. parapsilosis CMGB-YT was grown on yeast peptone (YP) media with 1% n-decane, n-dodecane, n-tetradecane, n-hexadecane, as well as commercial sunflower and olive oils. The production of the biosurfactant was evaluated using the emulsification index (E24%). The surface properties and emulsifying stability of the biosurfactant were determined. The effect of the biosurfactant on the cell growth of two strains of Rhodotorula mucilaginosa and on their removal capacity of lead (0.032 g/L) and cadmium (0.030 g/L) ions from synthetic wastewater were also studied. The antimicrobial potential of 20 mg/mL and 40 mg/mL biosurfactant was established in the presence of pathogenic Candida krusei strains. C. parapsilosis CMGB-YT assimilated n-hexadecane with good rates over 216 h and produced an anionic glycolipidic biosurfactant with stable E24% towards long-chain carbon compounds at different temperatures, with an alkaline pH and high salinity (10% NaCl). The biosurfactant reduced the surface tension to 53.58 ± 0.42 mN/m, while the critical micellar concentration (CMC) was reached at 4.2% biosurfactant. The crude biosurfactant (5%) enhanced R. mucilaginosa growth in heavy metal-contaminated wastewater, increased chemical oxygen demand (COD) removal of up to 80%, and improved Cd2+ removal by 10%. Additionally, the concentrated biosurfactant effectively prevented Candida krusei biofilm formation. 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source MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Antimicrobial activity
Aquatic resources
Assimilation
Bioremediation
Biosurfactants
Cadmium
Candida krusei
Candida parapsilosis
Carbon
Carbon compounds
Chemical oxygen demand
Dodecane
Emulsification
Enzymes
Fatty acids
Fungi
Genomes
Heavy metals
Hexadecane
Hydrocarbons
Hydrophobicity
Metabolism
Microbial contamination
Microorganisms
Olive oil
Peptones
Purification
Romania
Sewage
Sodium chloride
Substrates
Surface properties
Surface stability
Surface tension
Surfactants
Tetradecane
United Kingdom
Vegetable oils
Wastewater treatment
Water pollution
Yeast
title Candida parapsilosis CMGB-YT Biosurfactant for Treatment of Heavy Metal- and Microbial-Contaminated Wastewater
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