Staphylococcus aureus lytic bacteriophage: isolation and application evaluation
Staphylococcus aureus is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this rega...
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| Veröffentlicht in: | Journal für Verbraucherschutz und Lebensmittelsicherheit 2024, Vol.19 (2), p.235-243 |
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| creator | Lambuk, Fatmawati Mazlan, Nurzafirah Lesen, Dalene Nillian, Elexson Thung, Tze Young |
| description | Staphylococcus aureus
is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efficacy in combating
S. aureus
biofilms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both
S. aureus
and methicillin-resistant
S. aureus
strains. Belonging to the
Myoviridae
family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and effectively degraded established biofilms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in significant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm
2
, and 3.2 log CFU/cm
2
, respectively). These findings underscore the potential of phage mSA4 as a natural biocontrol agent against
S. aureus
.
Graphical abstract |
| doi_str_mv | 10.1007/s00003-024-01479-8 |
| format | Article |
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is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efficacy in combating
S. aureus
biofilms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both
S. aureus
and methicillin-resistant
S. aureus
strains. Belonging to the
Myoviridae
family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and effectively degraded established biofilms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in significant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm
2
, and 3.2 log CFU/cm
2
, respectively). These findings underscore the potential of phage mSA4 as a natural biocontrol agent against
S. aureus
.
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is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efficacy in combating
S. aureus
biofilms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both
S. aureus
and methicillin-resistant
S. aureus
strains. Belonging to the
Myoviridae
family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and effectively degraded established biofilms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in significant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm
2
, and 3.2 log CFU/cm
2
, respectively). These findings underscore the potential of phage mSA4 as a natural biocontrol agent against
S. aureus
.
Graphical abstract</description><subject>Agriculture</subject><subject>Biofilms</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Burst size</subject><subject>Chemistry/Food Science</subject><subject>Drug resistance</subject><subject>Food</subject><subject>Food contamination</subject><subject>Food poisoning</subject><subject>Food Science</subject><subject>Foodborne pathogens</subject><subject>Host range</subject><subject>Latent period</subject><subject>Lettuce</subject><subject>Life Sciences</subject><subject>Methicillin</subject><subject>Performance evaluation</subject><subject>Phages</subject><subject>Plant Genetics and Genomics</subject><subject>Research Article</subject><subject>Staphylococcus aureus</subject><issn>1661-5751</issn><issn>1661-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYMouK5-AU8Fz9WZpE1Sb7L4Dxb2oJ7DNJvudqlNTVphv711q3hzLm8G3nsDP8YuEa4RQN1EGEekwLMUMFNFqo_YDKXENNdSHf_uKsdTdhbjDiBXWokZW7301G33jbfe2iEmNAQ3SrPva5uUZHsXat9taeNukzr6hvratwm164S6rqntdLtPaobDes5OKmqiu_jROXt7uH9dPKXL1ePz4m6ZWq6gT6VAXhXCceK6wAwVOC5Qg6hUoQrKBc_KUkmnEQlkTmUG6xyFsAhakpVizq6m3i74j8HF3uz8ENrxpRGgkOeKQza6-OSywccYXGW6UL9T2BsE8w3OTODMCM4cwBk9hsQUiqO53bjwV_1P6gu6pXAQ</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Lambuk, Fatmawati</creator><creator>Mazlan, Nurzafirah</creator><creator>Lesen, Dalene</creator><creator>Nillian, Elexson</creator><creator>Thung, Tze Young</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-2275-1422</orcidid></search><sort><creationdate>2024</creationdate><title>Staphylococcus aureus lytic bacteriophage: isolation and application evaluation</title><author>Lambuk, Fatmawati ; Mazlan, Nurzafirah ; Lesen, Dalene ; Nillian, Elexson ; Thung, Tze Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-6312f93e2a28914170e231803f7979a5324bb76e811a065ab40d5133c1086ac63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agriculture</topic><topic>Biofilms</topic><topic>Biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Burst size</topic><topic>Chemistry/Food Science</topic><topic>Drug resistance</topic><topic>Food</topic><topic>Food contamination</topic><topic>Food poisoning</topic><topic>Food Science</topic><topic>Foodborne pathogens</topic><topic>Host range</topic><topic>Latent period</topic><topic>Lettuce</topic><topic>Life Sciences</topic><topic>Methicillin</topic><topic>Performance evaluation</topic><topic>Phages</topic><topic>Plant Genetics and Genomics</topic><topic>Research Article</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lambuk, Fatmawati</creatorcontrib><creatorcontrib>Mazlan, Nurzafirah</creatorcontrib><creatorcontrib>Lesen, Dalene</creatorcontrib><creatorcontrib>Nillian, Elexson</creatorcontrib><creatorcontrib>Thung, Tze Young</creatorcontrib><collection>CrossRef</collection><jtitle>Journal für Verbraucherschutz und Lebensmittelsicherheit</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lambuk, Fatmawati</au><au>Mazlan, Nurzafirah</au><au>Lesen, Dalene</au><au>Nillian, Elexson</au><au>Thung, Tze Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcus aureus lytic bacteriophage: isolation and application evaluation</atitle><jtitle>Journal für Verbraucherschutz und Lebensmittelsicherheit</jtitle><stitle>J Consum Prot Food Saf</stitle><date>2024</date><risdate>2024</risdate><volume>19</volume><issue>2</issue><spage>235</spage><epage>243</epage><pages>235-243</pages><issn>1661-5751</issn><eissn>1661-5867</eissn><abstract>Staphylococcus aureus
is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efficacy in combating
S. aureus
biofilms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both
S. aureus
and methicillin-resistant
S. aureus
strains. Belonging to the
Myoviridae
family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and effectively degraded established biofilms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in significant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm
2
, and 3.2 log CFU/cm
2
, respectively). These findings underscore the potential of phage mSA4 as a natural biocontrol agent against
S. aureus
.
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| subjects | Agriculture Biofilms Biological control Biomedical and Life Sciences Biotechnology Burst size Chemistry/Food Science Drug resistance Food Food contamination Food poisoning Food Science Foodborne pathogens Host range Latent period Lettuce Life Sciences Methicillin Performance evaluation Phages Plant Genetics and Genomics Research Article Staphylococcus aureus |
| title | Staphylococcus aureus lytic bacteriophage: isolation and application evaluation |
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