A facile and simple synthesis of a cytotoxic tocotrienol-based nanoemulsion against MCF-7 and A549 cancer cell lines
Tocotrienol is a subfamily of natural vitamin E with multiple biological activities, including antioxidants, antiproliferative, proapoptotic, antiangiogenic, and anti-inflammatory properties. Despite numerous biological activities, the application of tocotrienol is hampered by its poor solubility, r...
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| Veröffentlicht in: | Colloid and polymer science 2024-07, Vol.302 (7), p.1025-1035 |
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| creator | Rosyidah, A’liyatur Pratiwi, Riyona Desvy Muttaqien, Sjaikhurrizal El Rahmawati, Siti Irma Bayu, Asep Tan, Sui Ling Janet Gustini, Nunik Ahmadi, Peni Putra, Masteria Yunovilsa |
| description | Tocotrienol is a subfamily of natural vitamin E with multiple biological activities, including antioxidants, antiproliferative, proapoptotic, antiangiogenic, and anti-inflammatory properties. Despite numerous biological activities, the application of tocotrienol is hampered by its poor solubility, resulting in low bioavailability, and in turn, limits its therapeutic effectivity. To address these limitations, the present study focuses on the development of tocotrienol nanoemulsion, followed by an in vitro anticancer evaluation of the formula. The tocotrienol nanoemulsion was prepared by a combination of high-speed homogenization with ultrasonication, using food-grade canola oil and Tween 80 as oil phases and surfactants, respectively. The formulated nanoemulsion observed an encapsulation efficiency of 90.26% with particle size and zeta potential of 145 ± 0.06 nm and −25.27 ± 0.01 mV, respectively. The FTIR spectra show no interference between the active compounds and the excipients, indicating that tocotrienol was successfully loaded into the nanoemulsion. Besides, the tocotrienol nanoemulsions demonstrated higher antioxidant ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity than the free form of tocotrienol at the same concentration (
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| doi_str_mv | 10.1007/s00396-024-05245-y |
| format | Article |
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p
< 0.05). In the in vitro cytotoxicity studies, there was a decrease in cell viability observed against MCF-7 breast and A549 lung cancer cell lines for tocotrienol nanoemulsion. Overall, it suggests that nanoemulsion-based natural component delivery systems have substantial implications in developing and designing encapsulated biologically active systems. The potent cytotoxicity of tocotrienol-loaded nanoemulsion under aqueous phases provides insight into the development of nanoemulsion systems for enhancing the bioavailability and activity of tocotrienol as well as other lipophilic compounds in water systems, particularly for anticancer therapeutic.
Graphical Abstract</description><identifier>ISSN: 0303-402X</identifier><identifier>EISSN: 1435-1536</identifier><identifier>DOI: 10.1007/s00396-024-05245-y</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antioxidants ; Bioavailability ; Biological properties ; Cancer ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Complex Fluids and Microfluidics ; Cytotoxicity ; Encapsulation ; Food Science ; Free form ; Nanoemulsions ; Nanotechnology and Microengineering ; Physical Chemistry ; Polymer Sciences ; Scavenging ; Soft and Granular Matter ; Sulfonic acid ; Toxicity ; Zeta potential</subject><ispartof>Colloid and polymer science, 2024-07, Vol.302 (7), p.1025-1035</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-babd76eca4d33195b2b1561718bc151a78d05a3bdd05a2403857b542f93d76673</cites><orcidid>0000-0001-5317-466X ; 0000-0003-0865-3420 ; 0000-0003-3313-9909 ; 0000-0001-5376-7860 ; 0000-0002-5693-0501 ; 0000-0001-6249-2424 ; 0000-0002-9879-3293 ; 0000-0001-9239-3179 ; 0000-0001-8427-4364</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00396-024-05245-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00396-024-05245-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Rosyidah, A’liyatur</creatorcontrib><creatorcontrib>Pratiwi, Riyona Desvy</creatorcontrib><creatorcontrib>Muttaqien, Sjaikhurrizal El</creatorcontrib><creatorcontrib>Rahmawati, Siti Irma</creatorcontrib><creatorcontrib>Bayu, Asep</creatorcontrib><creatorcontrib>Tan, Sui Ling Janet</creatorcontrib><creatorcontrib>Gustini, Nunik</creatorcontrib><creatorcontrib>Ahmadi, Peni</creatorcontrib><creatorcontrib>Putra, Masteria Yunovilsa</creatorcontrib><title>A facile and simple synthesis of a cytotoxic tocotrienol-based nanoemulsion against MCF-7 and A549 cancer cell lines</title><title>Colloid and polymer science</title><addtitle>Colloid Polym Sci</addtitle><description>Tocotrienol is a subfamily of natural vitamin E with multiple biological activities, including antioxidants, antiproliferative, proapoptotic, antiangiogenic, and anti-inflammatory properties. Despite numerous biological activities, the application of tocotrienol is hampered by its poor solubility, resulting in low bioavailability, and in turn, limits its therapeutic effectivity. To address these limitations, the present study focuses on the development of tocotrienol nanoemulsion, followed by an in vitro anticancer evaluation of the formula. The tocotrienol nanoemulsion was prepared by a combination of high-speed homogenization with ultrasonication, using food-grade canola oil and Tween 80 as oil phases and surfactants, respectively. The formulated nanoemulsion observed an encapsulation efficiency of 90.26% with particle size and zeta potential of 145 ± 0.06 nm and −25.27 ± 0.01 mV, respectively. The FTIR spectra show no interference between the active compounds and the excipients, indicating that tocotrienol was successfully loaded into the nanoemulsion. Besides, the tocotrienol nanoemulsions demonstrated higher antioxidant ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity than the free form of tocotrienol at the same concentration (
p
< 0.05). In the in vitro cytotoxicity studies, there was a decrease in cell viability observed against MCF-7 breast and A549 lung cancer cell lines for tocotrienol nanoemulsion. Overall, it suggests that nanoemulsion-based natural component delivery systems have substantial implications in developing and designing encapsulated biologically active systems. The potent cytotoxicity of tocotrienol-loaded nanoemulsion under aqueous phases provides insight into the development of nanoemulsion systems for enhancing the bioavailability and activity of tocotrienol as well as other lipophilic compounds in water systems, particularly for anticancer therapeutic.
Graphical Abstract</description><subject>Antioxidants</subject><subject>Bioavailability</subject><subject>Biological properties</subject><subject>Cancer</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Complex Fluids and Microfluidics</subject><subject>Cytotoxicity</subject><subject>Encapsulation</subject><subject>Food Science</subject><subject>Free form</subject><subject>Nanoemulsions</subject><subject>Nanotechnology and Microengineering</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>Scavenging</subject><subject>Soft and Granular Matter</subject><subject>Sulfonic acid</subject><subject>Toxicity</subject><subject>Zeta potential</subject><issn>0303-402X</issn><issn>1435-1536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEURYMoWKt_wFXAdfTlczrLUqwKihsFdyGTydSUaVKTFJx_77QV3Lm6b3HPfXAQuqZwSwGquwzAa0WACQKSCUmGEzShgktCJVenaAIcOBHAPs7RRc5rABC1UhNU5rgz1vcOm9Di7Dfb8cxDKJ8u-4xjhw22Q4klfnuLS7SxJO9C7EljsmtxMCG6za7PPgZsVsaHXPDLYkmqw-BcihpbE6xL2Lq-x70PLl-is8702V395hS9L-_fFo_k-fXhaTF_JpZVUMYPTVspZ41oOae1bFhDpaIVnTWWSmqqWQvS8KbdBxPAZ7JqpGBdzUdOVXyKbo672xS_di4XvY67FMaXmoOqVS1YLcYWO7Zsijkn1-lt8huTBk1B7-3qo1092tUHu3oYIX6E8lgOK5f-pv-hfgDGbH1F</recordid><startdate>20240701</startdate><enddate>20240701</enddate><creator>Rosyidah, A’liyatur</creator><creator>Pratiwi, Riyona Desvy</creator><creator>Muttaqien, Sjaikhurrizal El</creator><creator>Rahmawati, Siti Irma</creator><creator>Bayu, Asep</creator><creator>Tan, Sui Ling Janet</creator><creator>Gustini, Nunik</creator><creator>Ahmadi, Peni</creator><creator>Putra, Masteria Yunovilsa</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0001-5317-466X</orcidid><orcidid>https://orcid.org/0000-0003-0865-3420</orcidid><orcidid>https://orcid.org/0000-0003-3313-9909</orcidid><orcidid>https://orcid.org/0000-0001-5376-7860</orcidid><orcidid>https://orcid.org/0000-0002-5693-0501</orcidid><orcidid>https://orcid.org/0000-0001-6249-2424</orcidid><orcidid>https://orcid.org/0000-0002-9879-3293</orcidid><orcidid>https://orcid.org/0000-0001-9239-3179</orcidid><orcidid>https://orcid.org/0000-0001-8427-4364</orcidid></search><sort><creationdate>20240701</creationdate><title>A facile and simple synthesis of a cytotoxic tocotrienol-based nanoemulsion against MCF-7 and A549 cancer cell lines</title><author>Rosyidah, A’liyatur ; Pratiwi, Riyona Desvy ; Muttaqien, Sjaikhurrizal El ; Rahmawati, Siti Irma ; Bayu, Asep ; Tan, Sui Ling Janet ; Gustini, Nunik ; Ahmadi, Peni ; Putra, Masteria Yunovilsa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-babd76eca4d33195b2b1561718bc151a78d05a3bdd05a2403857b542f93d76673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antioxidants</topic><topic>Bioavailability</topic><topic>Biological properties</topic><topic>Cancer</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Complex Fluids and Microfluidics</topic><topic>Cytotoxicity</topic><topic>Encapsulation</topic><topic>Food Science</topic><topic>Free form</topic><topic>Nanoemulsions</topic><topic>Nanotechnology and Microengineering</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>Scavenging</topic><topic>Soft and Granular Matter</topic><topic>Sulfonic acid</topic><topic>Toxicity</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosyidah, A’liyatur</creatorcontrib><creatorcontrib>Pratiwi, Riyona Desvy</creatorcontrib><creatorcontrib>Muttaqien, Sjaikhurrizal El</creatorcontrib><creatorcontrib>Rahmawati, Siti Irma</creatorcontrib><creatorcontrib>Bayu, Asep</creatorcontrib><creatorcontrib>Tan, Sui Ling Janet</creatorcontrib><creatorcontrib>Gustini, Nunik</creatorcontrib><creatorcontrib>Ahmadi, Peni</creatorcontrib><creatorcontrib>Putra, Masteria Yunovilsa</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Colloid and polymer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosyidah, A’liyatur</au><au>Pratiwi, Riyona Desvy</au><au>Muttaqien, Sjaikhurrizal El</au><au>Rahmawati, Siti Irma</au><au>Bayu, Asep</au><au>Tan, Sui Ling Janet</au><au>Gustini, Nunik</au><au>Ahmadi, Peni</au><au>Putra, Masteria Yunovilsa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A facile and simple synthesis of a cytotoxic tocotrienol-based nanoemulsion against MCF-7 and A549 cancer cell lines</atitle><jtitle>Colloid and polymer science</jtitle><stitle>Colloid Polym Sci</stitle><date>2024-07-01</date><risdate>2024</risdate><volume>302</volume><issue>7</issue><spage>1025</spage><epage>1035</epage><pages>1025-1035</pages><issn>0303-402X</issn><eissn>1435-1536</eissn><abstract>Tocotrienol is a subfamily of natural vitamin E with multiple biological activities, including antioxidants, antiproliferative, proapoptotic, antiangiogenic, and anti-inflammatory properties. Despite numerous biological activities, the application of tocotrienol is hampered by its poor solubility, resulting in low bioavailability, and in turn, limits its therapeutic effectivity. To address these limitations, the present study focuses on the development of tocotrienol nanoemulsion, followed by an in vitro anticancer evaluation of the formula. The tocotrienol nanoemulsion was prepared by a combination of high-speed homogenization with ultrasonication, using food-grade canola oil and Tween 80 as oil phases and surfactants, respectively. The formulated nanoemulsion observed an encapsulation efficiency of 90.26% with particle size and zeta potential of 145 ± 0.06 nm and −25.27 ± 0.01 mV, respectively. The FTIR spectra show no interference between the active compounds and the excipients, indicating that tocotrienol was successfully loaded into the nanoemulsion. Besides, the tocotrienol nanoemulsions demonstrated higher antioxidant ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity than the free form of tocotrienol at the same concentration (
p
< 0.05). In the in vitro cytotoxicity studies, there was a decrease in cell viability observed against MCF-7 breast and A549 lung cancer cell lines for tocotrienol nanoemulsion. Overall, it suggests that nanoemulsion-based natural component delivery systems have substantial implications in developing and designing encapsulated biologically active systems. The potent cytotoxicity of tocotrienol-loaded nanoemulsion under aqueous phases provides insight into the development of nanoemulsion systems for enhancing the bioavailability and activity of tocotrienol as well as other lipophilic compounds in water systems, particularly for anticancer therapeutic.
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| ispartof | Colloid and polymer science, 2024-07, Vol.302 (7), p.1025-1035 |
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| subjects | Antioxidants Bioavailability Biological properties Cancer Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Complex Fluids and Microfluidics Cytotoxicity Encapsulation Food Science Free form Nanoemulsions Nanotechnology and Microengineering Physical Chemistry Polymer Sciences Scavenging Soft and Granular Matter Sulfonic acid Toxicity Zeta potential |
| title | A facile and simple synthesis of a cytotoxic tocotrienol-based nanoemulsion against MCF-7 and A549 cancer cell lines |
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