Antibody-labeled gold nanoparticle based resonance Rayleigh scattering detection of S100B
Traumatic brain injury (TBI) is a sudden brain injury due to an external force that causes a large number of deaths and permanent disabilities every year. S100B has been recognized as a potential objective quantitative biomarker for screening the prognosis of TBI and severe head injury. In this arti...
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| Veröffentlicht in: | Analytical methods 2024-05, Vol.16 (19), p.374-38 |
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| description | Traumatic brain injury (TBI) is a sudden brain injury due to an external force that causes a large number of deaths and permanent disabilities every year. S100B has been recognized as a potential objective quantitative biomarker for screening the prognosis of TBI and severe head injury. In this article, an anti-S100B monoclonal antibody was immobilized on cysteamine (Cy) functionalized gold nanoparticles (AuNPs) by EDC-NHS chemistry, which enabled S100B resonance Rayleigh scattering (RRS) detection based on antibody-labeled gold nanoparticles. The prepared conjugates were characterized by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Based on the specific binding of the antibody and antigen, the RRS intensities at 381 nm and 541 nm wavelengths were significantly enhanced, and thus a dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established. The scattering intensity of the two overlapping peaks was proportional to the concentration of S100B in the range of 0.05-4.5 ng mL
−1
with a detection limit of 0.002 ng mL
−1
. The proposed DWO-RRS method is time-saving, simple, sensitive, and can be used to determine the concentration of S100B in human serum with satisfactory results, which has a promising application in the early diagnosis of TBI.
A dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established and can be used to determine the concentration of S100B in human serum with satisfactory results. |
| doi_str_mv | 10.1039/d4ay00335g |
| format | Article |
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−1
with a detection limit of 0.002 ng mL
−1
. The proposed DWO-RRS method is time-saving, simple, sensitive, and can be used to determine the concentration of S100B in human serum with satisfactory results, which has a promising application in the early diagnosis of TBI.
A dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established and can be used to determine the concentration of S100B in human serum with satisfactory results.</description><identifier>ISSN: 1759-9660</identifier><identifier>ISSN: 1759-9679</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/d4ay00335g</identifier><identifier>PMID: 38683678</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Antibodies ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; Biomarkers ; Brain ; Brain Injuries, Traumatic - blood ; Brain Injuries, Traumatic - diagnostic imaging ; Cysteamine ; Dynamic Light Scattering - methods ; Electron microscopy ; Electrophoresis ; Fourier transforms ; Gels ; Gold ; Gold - chemistry ; Head injuries ; Humans ; Infrared spectroscopy ; Light scattering ; Limit of Detection ; Metal Nanoparticles - chemistry ; Monoclonal antibodies ; Nanoparticles ; Photon correlation spectroscopy ; Polyacrylamide ; Rayleigh scattering ; Resonance ; Resonance scattering ; S100 Calcium Binding Protein beta Subunit - analysis ; S100 Calcium Binding Protein beta Subunit - blood ; S100b protein ; Scattering, Radiation ; Sodium ; Sodium dodecyl sulfate ; Sodium lauryl sulfate ; Sulfates ; Transmission electron microscopy ; Traumatic brain injury ; Wavelength ; Wavelengths</subject><ispartof>Analytical methods, 2024-05, Vol.16 (19), p.374-38</ispartof><rights>Copyright Royal Society of Chemistry 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-16ed06e554eea608e735fd1f565bc5f297fd1cb619c7ba9d8c0a9c85a6c3ff163</citedby><cites>FETCH-LOGICAL-c378t-16ed06e554eea608e735fd1f565bc5f297fd1cb619c7ba9d8c0a9c85a6c3ff163</cites><orcidid>0000-0001-8794-8491</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38683678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tiantian, Wang</creatorcontrib><creatorcontrib>Yonghui, Wang</creatorcontrib><creatorcontrib>Junbo, Li</creatorcontrib><title>Antibody-labeled gold nanoparticle based resonance Rayleigh scattering detection of S100B</title><title>Analytical methods</title><addtitle>Anal Methods</addtitle><description>Traumatic brain injury (TBI) is a sudden brain injury due to an external force that causes a large number of deaths and permanent disabilities every year. S100B has been recognized as a potential objective quantitative biomarker for screening the prognosis of TBI and severe head injury. In this article, an anti-S100B monoclonal antibody was immobilized on cysteamine (Cy) functionalized gold nanoparticles (AuNPs) by EDC-NHS chemistry, which enabled S100B resonance Rayleigh scattering (RRS) detection based on antibody-labeled gold nanoparticles. The prepared conjugates were characterized by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Based on the specific binding of the antibody and antigen, the RRS intensities at 381 nm and 541 nm wavelengths were significantly enhanced, and thus a dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established. The scattering intensity of the two overlapping peaks was proportional to the concentration of S100B in the range of 0.05-4.5 ng mL
−1
with a detection limit of 0.002 ng mL
−1
. The proposed DWO-RRS method is time-saving, simple, sensitive, and can be used to determine the concentration of S100B in human serum with satisfactory results, which has a promising application in the early diagnosis of TBI.
A dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established and can be used to determine the concentration of S100B in human serum with satisfactory results.</description><subject>Antibodies</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biomarkers</subject><subject>Brain</subject><subject>Brain Injuries, Traumatic - blood</subject><subject>Brain Injuries, Traumatic - diagnostic imaging</subject><subject>Cysteamine</subject><subject>Dynamic Light Scattering - methods</subject><subject>Electron microscopy</subject><subject>Electrophoresis</subject><subject>Fourier transforms</subject><subject>Gels</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Head injuries</subject><subject>Humans</subject><subject>Infrared spectroscopy</subject><subject>Light scattering</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Monoclonal antibodies</subject><subject>Nanoparticles</subject><subject>Photon correlation spectroscopy</subject><subject>Polyacrylamide</subject><subject>Rayleigh scattering</subject><subject>Resonance</subject><subject>Resonance scattering</subject><subject>S100 Calcium Binding Protein beta Subunit - analysis</subject><subject>S100 Calcium Binding Protein beta Subunit - blood</subject><subject>S100b protein</subject><subject>Scattering, Radiation</subject><subject>Sodium</subject><subject>Sodium dodecyl sulfate</subject><subject>Sodium lauryl sulfate</subject><subject>Sulfates</subject><subject>Transmission electron microscopy</subject><subject>Traumatic brain injury</subject><subject>Wavelength</subject><subject>Wavelengths</subject><issn>1759-9660</issn><issn>1759-9679</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c9LwzAUB_AgipvTi3el4EWEarI0aXKcU6cwEPxx2KmkyWvt6JqZtIf-90Y3J3hK8t6Hx-MbhE4JviaYyhuTqB5jSlm5h4YkZTKWPJX7uzvHA3Tk_RJjLiknh2hABReUp2KIFpOmrXJr-rhWOdRgotLWJmpUY9fKtZWuIcqVD3UH3oayhuhF9TVU5UfktWpbcFVTRgZa0G1lm8gW0SvB-PYYHRSq9nCyPUfo_eH-bfoYz59nT9PJPNY0FW1MOBjMgbEEQHEsIKWsMKRgnOWaFWOZhpfOOZE6zZU0QmMltWCKa1oUhNMRutzMXTv72YFvs1XlNdS1asB2PqM4kemYEkYDvfhHl7ZzTdguqLBBIphIgrraKO2s9w6KbO2qlXJ9RnD2HXh2l0wWP4HPAj7fjuzyFZgd_U04gLMNcF7vun8_Rr8AmHCFFw</recordid><startdate>20240516</startdate><enddate>20240516</enddate><creator>Tiantian, Wang</creator><creator>Yonghui, Wang</creator><creator>Junbo, Li</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8794-8491</orcidid></search><sort><creationdate>20240516</creationdate><title>Antibody-labeled gold nanoparticle based resonance Rayleigh scattering detection of S100B</title><author>Tiantian, Wang ; Yonghui, Wang ; Junbo, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-16ed06e554eea608e735fd1f565bc5f297fd1cb619c7ba9d8c0a9c85a6c3ff163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibodies</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biomarkers</topic><topic>Brain</topic><topic>Brain Injuries, Traumatic - blood</topic><topic>Brain Injuries, Traumatic - diagnostic imaging</topic><topic>Cysteamine</topic><topic>Dynamic Light Scattering - methods</topic><topic>Electron microscopy</topic><topic>Electrophoresis</topic><topic>Fourier transforms</topic><topic>Gels</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Head injuries</topic><topic>Humans</topic><topic>Infrared spectroscopy</topic><topic>Light scattering</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Monoclonal antibodies</topic><topic>Nanoparticles</topic><topic>Photon correlation spectroscopy</topic><topic>Polyacrylamide</topic><topic>Rayleigh scattering</topic><topic>Resonance</topic><topic>Resonance scattering</topic><topic>S100 Calcium Binding Protein beta Subunit - analysis</topic><topic>S100 Calcium Binding Protein beta Subunit - blood</topic><topic>S100b protein</topic><topic>Scattering, Radiation</topic><topic>Sodium</topic><topic>Sodium dodecyl sulfate</topic><topic>Sodium lauryl sulfate</topic><topic>Sulfates</topic><topic>Transmission electron microscopy</topic><topic>Traumatic brain injury</topic><topic>Wavelength</topic><topic>Wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tiantian, Wang</creatorcontrib><creatorcontrib>Yonghui, Wang</creatorcontrib><creatorcontrib>Junbo, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tiantian, Wang</au><au>Yonghui, Wang</au><au>Junbo, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibody-labeled gold nanoparticle based resonance Rayleigh scattering detection of S100B</atitle><jtitle>Analytical methods</jtitle><addtitle>Anal Methods</addtitle><date>2024-05-16</date><risdate>2024</risdate><volume>16</volume><issue>19</issue><spage>374</spage><epage>38</epage><pages>374-38</pages><issn>1759-9660</issn><issn>1759-9679</issn><eissn>1759-9679</eissn><abstract>Traumatic brain injury (TBI) is a sudden brain injury due to an external force that causes a large number of deaths and permanent disabilities every year. S100B has been recognized as a potential objective quantitative biomarker for screening the prognosis of TBI and severe head injury. In this article, an anti-S100B monoclonal antibody was immobilized on cysteamine (Cy) functionalized gold nanoparticles (AuNPs) by EDC-NHS chemistry, which enabled S100B resonance Rayleigh scattering (RRS) detection based on antibody-labeled gold nanoparticles. The prepared conjugates were characterized by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Based on the specific binding of the antibody and antigen, the RRS intensities at 381 nm and 541 nm wavelengths were significantly enhanced, and thus a dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established. The scattering intensity of the two overlapping peaks was proportional to the concentration of S100B in the range of 0.05-4.5 ng mL
−1
with a detection limit of 0.002 ng mL
−1
. The proposed DWO-RRS method is time-saving, simple, sensitive, and can be used to determine the concentration of S100B in human serum with satisfactory results, which has a promising application in the early diagnosis of TBI.
A dual wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was established and can be used to determine the concentration of S100B in human serum with satisfactory results.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>38683678</pmid><doi>10.1039/d4ay00335g</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8794-8491</orcidid></addata></record> |
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| subjects | Antibodies Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Biomarkers Brain Brain Injuries, Traumatic - blood Brain Injuries, Traumatic - diagnostic imaging Cysteamine Dynamic Light Scattering - methods Electron microscopy Electrophoresis Fourier transforms Gels Gold Gold - chemistry Head injuries Humans Infrared spectroscopy Light scattering Limit of Detection Metal Nanoparticles - chemistry Monoclonal antibodies Nanoparticles Photon correlation spectroscopy Polyacrylamide Rayleigh scattering Resonance Resonance scattering S100 Calcium Binding Protein beta Subunit - analysis S100 Calcium Binding Protein beta Subunit - blood S100b protein Scattering, Radiation Sodium Sodium dodecyl sulfate Sodium lauryl sulfate Sulfates Transmission electron microscopy Traumatic brain injury Wavelength Wavelengths |
| title | Antibody-labeled gold nanoparticle based resonance Rayleigh scattering detection of S100B |
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