34 Successive Bouts of Exercise Influences the Inflammatory Cytokine Response in Thoroughbred Horses Regardless of Recovery Aid Supplementation

Abstract In equine sports, the rate of recovery after an exhaustive exercise session greatly affects the ability of the animal to perform subsequently, especially if the next exercise session occurs quickly. Carnitine participates in fatty acid delivery to the mitochondria and may facilitate muscle recovery post-exercise. The objective of the study was to determine the effects of a commercially available carnitine supplement (Renew, Platinum Performance, Buellton, CA) on adult The ability of Thoroughbred horses (16 geldings, 2 mares, 6.69 ± 0.49 years, 524.81 ± 10.07 kg, 5.66 ± 0.12 BCS) to perform successive bouts of exhaustive exercise. All horses participated in a moderate workload conditioning program for 6-weeks before testing. Horses received a ration of applesauce (30 mL, CON, n = 8) or ration of applesauce containing 19.6 g of the product (30 mL, AID, n = 8) 1 h before performing an incremental standardized exercise test (iSET) to exhaustion (day 1). Heart rate (HR) and gait biomechanics were recorded throughout the duration of the iSET. Blood was collected at 0, 10 min, 1, 4 and 6 h relative to exercise for the measurement of lactate and interleukin (IL) 1b, 8 and 10 mRNA content. for the measurement of lactate and interleukin (IL) 1b, IL8 and IL10 mRNA content. The AID was administered again at 1 h post-iSET after collection of the blood sample. All horses were rested for 24 h before repeating the iSET (day 3) with performance data and sample collection. The AID did not affect time to maximum HR, time to reach V200 or total gallop time during the iSET on day 1 or day 3. The rate of HR decline post iSET was slower (P < 0.05) for all horses on day 3 compared with day 1. Blood lactate concentration was not affected by AID or day. The right front limb posterior fetlock angle at full stance phase increased (P < 0.05) with speed for both CON and AID supplemented horses on day 1. Horses receiving AID demonstrated an increase (P < 0.05) in this measure at day 3 while no significant differences (P > 0.05) in posterior angle were detected in CON. Administration of AID did not affect whole blood IL1b, IL8 or IL10 mRNA content on day 1 or day 3. Consolidation of the data demonstrates increased (P < 0.05) expression of IL1b and IL8 mRNA during post-exercise recovery time on day 1 that was absent on day 3. Expression of IL10 was altered (P < 0.05) over time post-exercise on both days. Results of the experiment demonstrate that successive bouts of exercise affe