Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation
To identify the cause of mass mortality of adult Macrobrachium rosenbergii in a farm in Gaoyou City, Jiangsu Province, China, a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying M. rosenbergii and identified as Aeromonas dhakensis by purification culture, biochemical characte...
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| Veröffentlicht in: | Journal of oceanology and limnology 2024-03, Vol.42 (2), p.664-675 |
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| creator | Peng, Xin Tu, Haihui Yao, Xinyi Lan, Xuan Zhong, Zhenxiao Luo, Jinping Tang, Qiongying Yi, Shaokui Xia, Zhenglong Yang, Guoliang |
| description | To identify the cause of mass mortality of adult
Macrobrachium rosenbergii
in a farm in Gaoyou City, Jiangsu Province, China, a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying
M. rosenbergii
and identified as
Aeromonas dhakensis
by purification culture, biochemical characterization, and 16S rRNA and
gyrB
gene sequence analysis. The results of the challenge test revealed that the strain was highly pathogenic and the 50% lethal dose (LD
50
) in 72 h to
M. rosenbergii
was 1.54×10
5
CFU/mL. The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes, including
ascV, aexT, aer, act, lip, ompAI, gcaT, acg
, and
exu
, supporting the strong virulence of strain DKQ-1 to
M. rosenbergii
. Histopathological observation of the hepatopancreas, gills, and intestines indicated that DKQ-1 injection into
M. rosenbergii
could cause serious tissue damage, which further supported the strong virulence of this strain. In addition, a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics, resistant to 9 kinds of antibiotics, and had intermediate resistance to spectinomycin and kanamycin. This study is the first report of
A. dhakensis
isolated from
M. rosenbergii
and provided a reference for the pathogen identification of bacterial diseases in
M. rosenbergii
, and for the prevention and treatment caused by
A. dhakensis
. |
| doi_str_mv | 10.1007/s00343-023-3038-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_3040116084</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3040116084</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-9906c3994a6ab6571a0c9e5f0af57d11cb53f1fb5f736c71225fede9043e85d3</originalsourceid><addsrcrecordid>eNp1UctqwzAQNKWFhjQf0Jug17pdWZYfxxD6CKT0kruQ5VWi1JFSyQnkS_q7lZNATz3tzjAzyzJJck_hiQKUzwGA5SyFjKUMWJXCVTLK-IB4Rq_jDnWRcg7VbTIJYQMAGVQZcD5KfubBdbI3zj4S06LtjTbqgqVtycH4fYdWIVmhxUjJ7hhMIE6TnezXLrJGkSl6t3VWBtKu5RfaQWEs-ZDKu8ZLtTb7LfEuoG3Qr4w5Ra9N6N0ppHOreLQjrgnoD6frd8mNll3AyWWOk-Xry3L2ni4-3-az6SJVjBZ9WtdQKFbXuSxkU_CSSlA1cg1S87KlVDWcaaobrktWqJJmGdfYYg05w4q3bJw8nGN33n3vMfRi4_Y-PhkEgxwoLaDKo4qeVfGdEDxqsfNmK_1RUBBDA-LcgIgNiKEBAdGTnT0hau0K_V_y_6Zf-2SMfw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3040116084</pqid></control><display><type>article</type><title>Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation</title><source>Alma/SFX Local Collection</source><source>SpringerLink Journals - AutoHoldings</source><creator>Peng, Xin ; Tu, Haihui ; Yao, Xinyi ; Lan, Xuan ; Zhong, Zhenxiao ; Luo, Jinping ; Tang, Qiongying ; Yi, Shaokui ; Xia, Zhenglong ; Yang, Guoliang</creator><creatorcontrib>Peng, Xin ; Tu, Haihui ; Yao, Xinyi ; Lan, Xuan ; Zhong, Zhenxiao ; Luo, Jinping ; Tang, Qiongying ; Yi, Shaokui ; Xia, Zhenglong ; Yang, Guoliang</creatorcontrib><description>To identify the cause of mass mortality of adult
Macrobrachium rosenbergii
in a farm in Gaoyou City, Jiangsu Province, China, a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying
M. rosenbergii
and identified as
Aeromonas dhakensis
by purification culture, biochemical characterization, and 16S rRNA and
gyrB
gene sequence analysis. The results of the challenge test revealed that the strain was highly pathogenic and the 50% lethal dose (LD
50
) in 72 h to
M. rosenbergii
was 1.54×10
5
CFU/mL. The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes, including
ascV, aexT, aer, act, lip, ompAI, gcaT, acg
, and
exu
, supporting the strong virulence of strain DKQ-1 to
M. rosenbergii
. Histopathological observation of the hepatopancreas, gills, and intestines indicated that DKQ-1 injection into
M. rosenbergii
could cause serious tissue damage, which further supported the strong virulence of this strain. In addition, a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics, resistant to 9 kinds of antibiotics, and had intermediate resistance to spectinomycin and kanamycin. This study is the first report of
A. dhakensis
isolated from
M. rosenbergii
and provided a reference for the pathogen identification of bacterial diseases in
M. rosenbergii
, and for the prevention and treatment caused by
A. dhakensis
.</description><identifier>ISSN: 2096-5508</identifier><identifier>EISSN: 2523-3521</identifier><identifier>DOI: 10.1007/s00343-023-3038-0</identifier><language>eng</language><publisher>Heidelberg: Science Press</publisher><subject>Aeromonas dhakensis ; Antibiotics ; Bacterial diseases ; DNA topoisomerase ; Earth and Environmental Science ; Earth Sciences ; Genes ; Gills ; Hepatopancreas ; Histopathology ; Intestine ; Intestines ; Kanamycin ; Lethal dose ; Macrobrachium rosenbergii ; Mass mortality ; Mortality causes ; Oceanography ; Research Paper ; rRNA 16S ; Sequence analysis ; Spectinomycin ; Toxicity tests ; Virulence ; Water purification</subject><ispartof>Journal of oceanology and limnology, 2024-03, Vol.42 (2), p.664-675</ispartof><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2024</rights><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2024.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-9906c3994a6ab6571a0c9e5f0af57d11cb53f1fb5f736c71225fede9043e85d3</citedby><cites>FETCH-LOGICAL-c316t-9906c3994a6ab6571a0c9e5f0af57d11cb53f1fb5f736c71225fede9043e85d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00343-023-3038-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00343-023-3038-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Peng, Xin</creatorcontrib><creatorcontrib>Tu, Haihui</creatorcontrib><creatorcontrib>Yao, Xinyi</creatorcontrib><creatorcontrib>Lan, Xuan</creatorcontrib><creatorcontrib>Zhong, Zhenxiao</creatorcontrib><creatorcontrib>Luo, Jinping</creatorcontrib><creatorcontrib>Tang, Qiongying</creatorcontrib><creatorcontrib>Yi, Shaokui</creatorcontrib><creatorcontrib>Xia, Zhenglong</creatorcontrib><creatorcontrib>Yang, Guoliang</creatorcontrib><title>Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation</title><title>Journal of oceanology and limnology</title><addtitle>J. Ocean. Limnol</addtitle><description>To identify the cause of mass mortality of adult
Macrobrachium rosenbergii
in a farm in Gaoyou City, Jiangsu Province, China, a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying
M. rosenbergii
and identified as
Aeromonas dhakensis
by purification culture, biochemical characterization, and 16S rRNA and
gyrB
gene sequence analysis. The results of the challenge test revealed that the strain was highly pathogenic and the 50% lethal dose (LD
50
) in 72 h to
M. rosenbergii
was 1.54×10
5
CFU/mL. The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes, including
ascV, aexT, aer, act, lip, ompAI, gcaT, acg
, and
exu
, supporting the strong virulence of strain DKQ-1 to
M. rosenbergii
. Histopathological observation of the hepatopancreas, gills, and intestines indicated that DKQ-1 injection into
M. rosenbergii
could cause serious tissue damage, which further supported the strong virulence of this strain. In addition, a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics, resistant to 9 kinds of antibiotics, and had intermediate resistance to spectinomycin and kanamycin. This study is the first report of
A. dhakensis
isolated from
M. rosenbergii
and provided a reference for the pathogen identification of bacterial diseases in
M. rosenbergii
, and for the prevention and treatment caused by
A. dhakensis
.</description><subject>Aeromonas dhakensis</subject><subject>Antibiotics</subject><subject>Bacterial diseases</subject><subject>DNA topoisomerase</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Genes</subject><subject>Gills</subject><subject>Hepatopancreas</subject><subject>Histopathology</subject><subject>Intestine</subject><subject>Intestines</subject><subject>Kanamycin</subject><subject>Lethal dose</subject><subject>Macrobrachium rosenbergii</subject><subject>Mass mortality</subject><subject>Mortality causes</subject><subject>Oceanography</subject><subject>Research Paper</subject><subject>rRNA 16S</subject><subject>Sequence analysis</subject><subject>Spectinomycin</subject><subject>Toxicity tests</subject><subject>Virulence</subject><subject>Water purification</subject><issn>2096-5508</issn><issn>2523-3521</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1UctqwzAQNKWFhjQf0Jug17pdWZYfxxD6CKT0kruQ5VWi1JFSyQnkS_q7lZNATz3tzjAzyzJJck_hiQKUzwGA5SyFjKUMWJXCVTLK-IB4Rq_jDnWRcg7VbTIJYQMAGVQZcD5KfubBdbI3zj4S06LtjTbqgqVtycH4fYdWIVmhxUjJ7hhMIE6TnezXLrJGkSl6t3VWBtKu5RfaQWEs-ZDKu8ZLtTb7LfEuoG3Qr4w5Ra9N6N0ppHOreLQjrgnoD6frd8mNll3AyWWOk-Xry3L2ni4-3-az6SJVjBZ9WtdQKFbXuSxkU_CSSlA1cg1S87KlVDWcaaobrktWqJJmGdfYYg05w4q3bJw8nGN33n3vMfRi4_Y-PhkEgxwoLaDKo4qeVfGdEDxqsfNmK_1RUBBDA-LcgIgNiKEBAdGTnT0hau0K_V_y_6Zf-2SMfw</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Peng, Xin</creator><creator>Tu, Haihui</creator><creator>Yao, Xinyi</creator><creator>Lan, Xuan</creator><creator>Zhong, Zhenxiao</creator><creator>Luo, Jinping</creator><creator>Tang, Qiongying</creator><creator>Yi, Shaokui</creator><creator>Xia, Zhenglong</creator><creator>Yang, Guoliang</creator><general>Science Press</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QL</scope><scope>7SN</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H96</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20240301</creationdate><title>Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation</title><author>Peng, Xin ; Tu, Haihui ; Yao, Xinyi ; Lan, Xuan ; Zhong, Zhenxiao ; Luo, Jinping ; Tang, Qiongying ; Yi, Shaokui ; Xia, Zhenglong ; Yang, Guoliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-9906c3994a6ab6571a0c9e5f0af57d11cb53f1fb5f736c71225fede9043e85d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aeromonas dhakensis</topic><topic>Antibiotics</topic><topic>Bacterial diseases</topic><topic>DNA topoisomerase</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Genes</topic><topic>Gills</topic><topic>Hepatopancreas</topic><topic>Histopathology</topic><topic>Intestine</topic><topic>Intestines</topic><topic>Kanamycin</topic><topic>Lethal dose</topic><topic>Macrobrachium rosenbergii</topic><topic>Mass mortality</topic><topic>Mortality causes</topic><topic>Oceanography</topic><topic>Research Paper</topic><topic>rRNA 16S</topic><topic>Sequence analysis</topic><topic>Spectinomycin</topic><topic>Toxicity tests</topic><topic>Virulence</topic><topic>Water purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Xin</creatorcontrib><creatorcontrib>Tu, Haihui</creatorcontrib><creatorcontrib>Yao, Xinyi</creatorcontrib><creatorcontrib>Lan, Xuan</creatorcontrib><creatorcontrib>Zhong, Zhenxiao</creatorcontrib><creatorcontrib>Luo, Jinping</creatorcontrib><creatorcontrib>Tang, Qiongying</creatorcontrib><creatorcontrib>Yi, Shaokui</creatorcontrib><creatorcontrib>Xia, Zhenglong</creatorcontrib><creatorcontrib>Yang, Guoliang</creatorcontrib><collection>CrossRef</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of oceanology and limnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Xin</au><au>Tu, Haihui</au><au>Yao, Xinyi</au><au>Lan, Xuan</au><au>Zhong, Zhenxiao</au><au>Luo, Jinping</au><au>Tang, Qiongying</au><au>Yi, Shaokui</au><au>Xia, Zhenglong</au><au>Yang, Guoliang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation</atitle><jtitle>Journal of oceanology and limnology</jtitle><stitle>J. Ocean. Limnol</stitle><date>2024-03-01</date><risdate>2024</risdate><volume>42</volume><issue>2</issue><spage>664</spage><epage>675</epage><pages>664-675</pages><issn>2096-5508</issn><eissn>2523-3521</eissn><abstract>To identify the cause of mass mortality of adult
Macrobrachium rosenbergii
in a farm in Gaoyou City, Jiangsu Province, China, a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying
M. rosenbergii
and identified as
Aeromonas dhakensis
by purification culture, biochemical characterization, and 16S rRNA and
gyrB
gene sequence analysis. The results of the challenge test revealed that the strain was highly pathogenic and the 50% lethal dose (LD
50
) in 72 h to
M. rosenbergii
was 1.54×10
5
CFU/mL. The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes, including
ascV, aexT, aer, act, lip, ompAI, gcaT, acg
, and
exu
, supporting the strong virulence of strain DKQ-1 to
M. rosenbergii
. Histopathological observation of the hepatopancreas, gills, and intestines indicated that DKQ-1 injection into
M. rosenbergii
could cause serious tissue damage, which further supported the strong virulence of this strain. In addition, a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics, resistant to 9 kinds of antibiotics, and had intermediate resistance to spectinomycin and kanamycin. This study is the first report of
A. dhakensis
isolated from
M. rosenbergii
and provided a reference for the pathogen identification of bacterial diseases in
M. rosenbergii
, and for the prevention and treatment caused by
A. dhakensis
.</abstract><cop>Heidelberg</cop><pub>Science Press</pub><doi>10.1007/s00343-023-3038-0</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2096-5508 |
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| issn | 2096-5508 2523-3521 |
| language | eng |
| recordid | cdi_proquest_journals_3040116084 |
| source | Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Aeromonas dhakensis Antibiotics Bacterial diseases DNA topoisomerase Earth and Environmental Science Earth Sciences Genes Gills Hepatopancreas Histopathology Intestine Intestines Kanamycin Lethal dose Macrobrachium rosenbergii Mass mortality Mortality causes Oceanography Research Paper rRNA 16S Sequence analysis Spectinomycin Toxicity tests Virulence Water purification |
| title | Isolation, identification, and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation |
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