Metabolomics analysis reveals the protective effect of fructooligosaccharide on abnormal metabolism of liver in Megalobrama amblycephala induced by Aeromonas hydrophila
Fructooligosaccharide (FOS) is a prebiotic and it can be used as a substitute for antibiotics in the breeding industry. However, the ability of FOS as an antibiotic alternative to protect fish against Aeromonas hydrophila ( A . hydrophila ) infection remains largely unknown. The purpose of this stud...
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description | Fructooligosaccharide (FOS) is a prebiotic and it can be used as a substitute for antibiotics in the breeding industry. However, the ability of FOS as an antibiotic alternative to protect fish against
Aeromonas hydrophila
(
A
.
hydrophila
) infection remains largely unknown. The purpose of this study was to investigate the protective effect of FOS on the liver of fish infected with
A
.
hydrophila
by metabolomics. Three groups were set up including control group (G1),
A
.
hydrophila
group (G2), and
A
.
hydrophila
+FOS group (G3) in the experiment. LC-MS-based metabolomic method was used to study the changes of metabolites in the liver of
Megalobrama amblycephala
(
M
.
amblycephala
), and to identify different metabolites and related metabolic pathways. Metabolomic analysis indicated that fish infected with
A
.
hydrophila
caused alterations of metabolic profiles in liver. Specifically the
A
.
hydrophila
causes a significant reduction in lipid metabolism, led to oxidative stress, and energy expenditure. The specific metabolic pathways include taurine and hypotaurine metabolism, purine metabolism, and FoxO signaling pathway. The addition of FOS to the diet reversed the liver metabolic abnormalities caused by
A
.
hydrophila
. The expression of related metabolites acetyl phosphate,
d
-glyceric acid, 2′-deoxyinosine 5′-monophosphate, and adenosine 5′-monophosphate were significantly reversed by dietary FOS compared with
A
.
hydrophila
infection. These differential metabolites were mainly involved in lipid metabolism, oxidative stress, energy metabolism, inflammation. In conclusion, dietary FOS significantly improved liver metabolic abnormalities caused by
A
.
hydrophila
, our study provide new evidence supporting that FOS can be used to remit the abnormal metabolism of liver caused by
A
.
hydrophila.
Graphical abstract |
doi_str_mv | 10.1007/s10499-023-01264-8 |
format | Article |
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Aeromonas hydrophila
(
A
.
hydrophila
) infection remains largely unknown. The purpose of this study was to investigate the protective effect of FOS on the liver of fish infected with
A
.
hydrophila
by metabolomics. Three groups were set up including control group (G1),
A
.
hydrophila
group (G2), and
A
.
hydrophila
+FOS group (G3) in the experiment. LC-MS-based metabolomic method was used to study the changes of metabolites in the liver of
Megalobrama amblycephala
(
M
.
amblycephala
), and to identify different metabolites and related metabolic pathways. Metabolomic analysis indicated that fish infected with
A
.
hydrophila
caused alterations of metabolic profiles in liver. Specifically the
A
.
hydrophila
causes a significant reduction in lipid metabolism, led to oxidative stress, and energy expenditure. The specific metabolic pathways include taurine and hypotaurine metabolism, purine metabolism, and FoxO signaling pathway. The addition of FOS to the diet reversed the liver metabolic abnormalities caused by
A
.
hydrophila
. The expression of related metabolites acetyl phosphate,
d
-glyceric acid, 2′-deoxyinosine 5′-monophosphate, and adenosine 5′-monophosphate were significantly reversed by dietary FOS compared with
A
.
hydrophila
infection. These differential metabolites were mainly involved in lipid metabolism, oxidative stress, energy metabolism, inflammation. In conclusion, dietary FOS significantly improved liver metabolic abnormalities caused by
A
.
hydrophila
, our study provide new evidence supporting that FOS can be used to remit the abnormal metabolism of liver caused by
A
.
hydrophila.
Graphical abstract</description><identifier>ISSN: 0967-6120</identifier><identifier>EISSN: 1573-143X</identifier><identifier>DOI: 10.1007/s10499-023-01264-8</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Aeromonas hydrophila ; Antibiotics ; Biological stress ; Biomedical and Life Sciences ; Breeding ; Fish ; Freshwater & Marine Ecology ; Life Sciences ; Lipids ; Liver ; Malformations ; Megalobrama amblycephala ; Metabolism ; Metabolites ; Oxidative stress ; Phosphates ; Purines ; Zoology</subject><ispartof>Aquaculture international, 2024-04, Vol.32 (2), p.2185-2202</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-830ca5af0b707ebd125dc4e09cee2c8a9899debe2363b4a2f3a4d2cf2fe480073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10499-023-01264-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10499-023-01264-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Ma, Jianshuang</creatorcontrib><creatorcontrib>Xu, Ruiyi</creatorcontrib><creatorcontrib>Wang, Bingke</creatorcontrib><creatorcontrib>Qi, Qian</creatorcontrib><creatorcontrib>Zhang, Chunnuan</creatorcontrib><title>Metabolomics analysis reveals the protective effect of fructooligosaccharide on abnormal metabolism of liver in Megalobrama amblycephala induced by Aeromonas hydrophila</title><title>Aquaculture international</title><addtitle>Aquacult Int</addtitle><description>Fructooligosaccharide (FOS) is a prebiotic and it can be used as a substitute for antibiotics in the breeding industry. However, the ability of FOS as an antibiotic alternative to protect fish against
Aeromonas hydrophila
(
A
.
hydrophila
) infection remains largely unknown. The purpose of this study was to investigate the protective effect of FOS on the liver of fish infected with
A
.
hydrophila
by metabolomics. Three groups were set up including control group (G1),
A
.
hydrophila
group (G2), and
A
.
hydrophila
+FOS group (G3) in the experiment. LC-MS-based metabolomic method was used to study the changes of metabolites in the liver of
Megalobrama amblycephala
(
M
.
amblycephala
), and to identify different metabolites and related metabolic pathways. Metabolomic analysis indicated that fish infected with
A
.
hydrophila
caused alterations of metabolic profiles in liver. Specifically the
A
.
hydrophila
causes a significant reduction in lipid metabolism, led to oxidative stress, and energy expenditure. The specific metabolic pathways include taurine and hypotaurine metabolism, purine metabolism, and FoxO signaling pathway. The addition of FOS to the diet reversed the liver metabolic abnormalities caused by
A
.
hydrophila
. The expression of related metabolites acetyl phosphate,
d
-glyceric acid, 2′-deoxyinosine 5′-monophosphate, and adenosine 5′-monophosphate were significantly reversed by dietary FOS compared with
A
.
hydrophila
infection. These differential metabolites were mainly involved in lipid metabolism, oxidative stress, energy metabolism, inflammation. In conclusion, dietary FOS significantly improved liver metabolic abnormalities caused by
A
.
hydrophila
, our study provide new evidence supporting that FOS can be used to remit the abnormal metabolism of liver caused by
A
.
hydrophila.
Graphical abstract</description><subject>Aeromonas hydrophila</subject><subject>Antibiotics</subject><subject>Biological stress</subject><subject>Biomedical and Life Sciences</subject><subject>Breeding</subject><subject>Fish</subject><subject>Freshwater & Marine Ecology</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Liver</subject><subject>Malformations</subject><subject>Megalobrama amblycephala</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Oxidative stress</subject><subject>Phosphates</subject><subject>Purines</subject><subject>Zoology</subject><issn>0967-6120</issn><issn>1573-143X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc1qHDEQhEVIIJtNXiAnQc7jtKTZ-Tka45-ATS4J5CZamtaOjGa0lmYN80Z-zMiZQG45qUFfFd1VjH0WcCEA2q9ZQN33FUhVgZBNXXVv2E4cWlWJWv16y3bQN23VCAnv2YecHwFAtbXYsZcHWtDEECdvM8cZw5p95omeCUPmy0j8lOJCdvHPxMm5MvHouEtnu8QY_DFmtHbE5AficeZo5pgmDHzajH2eXvlQ5In7mT_QEUM0CSfkOJmwWjqNGLD8DWdLAzcrv6QUpzhj5uM6pHgafcCP7J0rG9Gnv--e_by5_nF1V91_v_12dXlfWdnCUnUKLB7QgWmhJTMIeRhsTdBbImk77Lu-H8iQVI0yNUqnsB6kddJR3ZUk1Z592XzL2U9nyot-jOdUcslageobWYKThZIbZVPMOZHTp-QnTKsWoF8b0VsjujSi_zSiuyJSmygXeD5S-mf9H9VvO9OUSA</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Ma, Jianshuang</creator><creator>Xu, Ruiyi</creator><creator>Wang, Bingke</creator><creator>Qi, Qian</creator><creator>Zhang, Chunnuan</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>20240401</creationdate><title>Metabolomics analysis reveals the protective effect of fructooligosaccharide on abnormal metabolism of liver in Megalobrama amblycephala induced by Aeromonas hydrophila</title><author>Ma, Jianshuang ; Xu, Ruiyi ; Wang, Bingke ; Qi, Qian ; Zhang, Chunnuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-830ca5af0b707ebd125dc4e09cee2c8a9899debe2363b4a2f3a4d2cf2fe480073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aeromonas hydrophila</topic><topic>Antibiotics</topic><topic>Biological stress</topic><topic>Biomedical and Life Sciences</topic><topic>Breeding</topic><topic>Fish</topic><topic>Freshwater & Marine Ecology</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Liver</topic><topic>Malformations</topic><topic>Megalobrama amblycephala</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Oxidative stress</topic><topic>Phosphates</topic><topic>Purines</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Jianshuang</creatorcontrib><creatorcontrib>Xu, Ruiyi</creatorcontrib><creatorcontrib>Wang, Bingke</creatorcontrib><creatorcontrib>Qi, Qian</creatorcontrib><creatorcontrib>Zhang, Chunnuan</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Aquaculture international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Jianshuang</au><au>Xu, Ruiyi</au><au>Wang, Bingke</au><au>Qi, Qian</au><au>Zhang, Chunnuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolomics analysis reveals the protective effect of fructooligosaccharide on abnormal metabolism of liver in Megalobrama amblycephala induced by Aeromonas hydrophila</atitle><jtitle>Aquaculture international</jtitle><stitle>Aquacult Int</stitle><date>2024-04-01</date><risdate>2024</risdate><volume>32</volume><issue>2</issue><spage>2185</spage><epage>2202</epage><pages>2185-2202</pages><issn>0967-6120</issn><eissn>1573-143X</eissn><abstract>Fructooligosaccharide (FOS) is a prebiotic and it can be used as a substitute for antibiotics in the breeding industry. However, the ability of FOS as an antibiotic alternative to protect fish against
Aeromonas hydrophila
(
A
.
hydrophila
) infection remains largely unknown. The purpose of this study was to investigate the protective effect of FOS on the liver of fish infected with
A
.
hydrophila
by metabolomics. Three groups were set up including control group (G1),
A
.
hydrophila
group (G2), and
A
.
hydrophila
+FOS group (G3) in the experiment. LC-MS-based metabolomic method was used to study the changes of metabolites in the liver of
Megalobrama amblycephala
(
M
.
amblycephala
), and to identify different metabolites and related metabolic pathways. Metabolomic analysis indicated that fish infected with
A
.
hydrophila
caused alterations of metabolic profiles in liver. Specifically the
A
.
hydrophila
causes a significant reduction in lipid metabolism, led to oxidative stress, and energy expenditure. The specific metabolic pathways include taurine and hypotaurine metabolism, purine metabolism, and FoxO signaling pathway. The addition of FOS to the diet reversed the liver metabolic abnormalities caused by
A
.
hydrophila
. The expression of related metabolites acetyl phosphate,
d
-glyceric acid, 2′-deoxyinosine 5′-monophosphate, and adenosine 5′-monophosphate were significantly reversed by dietary FOS compared with
A
.
hydrophila
infection. These differential metabolites were mainly involved in lipid metabolism, oxidative stress, energy metabolism, inflammation. In conclusion, dietary FOS significantly improved liver metabolic abnormalities caused by
A
.
hydrophila
, our study provide new evidence supporting that FOS can be used to remit the abnormal metabolism of liver caused by
A
.
hydrophila.
Graphical abstract</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s10499-023-01264-8</doi><tpages>18</tpages></addata></record> |
fulltext | fulltext |
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language | eng |
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source | SpringerLink Journals - AutoHoldings |
subjects | Aeromonas hydrophila Antibiotics Biological stress Biomedical and Life Sciences Breeding Fish Freshwater & Marine Ecology Life Sciences Lipids Liver Malformations Megalobrama amblycephala Metabolism Metabolites Oxidative stress Phosphates Purines Zoology |
title | Metabolomics analysis reveals the protective effect of fructooligosaccharide on abnormal metabolism of liver in Megalobrama amblycephala induced by Aeromonas hydrophila |
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