Solid-State Fermentation of Hyperactive Pectinase by the Novel Strain Aspergillus sp. CM96

Pectinase, a kind of hydrolase, mainly contains polygalacturonase, pectinase, and pectin lyase, which can hydrolyze pectin to generate galacturonide and is widely used in industry. At present, pectinase’s activity is still relatively low. Hyperactive pectinase was produced with solid-phase fermentat...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Processes 2024-03, Vol.12 (3), p.615
Hauptverfasser: Chen, Huiling, Wan, Meimei, Liu, Yang, Yang, Guanghua, Cai, Zhiqiang
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 3
container_start_page 615
container_title Processes
container_volume 12
creator Chen, Huiling
Wan, Meimei
Liu, Yang
Yang, Guanghua
Cai, Zhiqiang
description Pectinase, a kind of hydrolase, mainly contains polygalacturonase, pectinase, and pectin lyase, which can hydrolyze pectin to generate galacturonide and is widely used in industry. At present, pectinase’s activity is still relatively low. Hyperactive pectinase was produced with solid-phase fermentation and a tray bioreactor using the novel strain Aspergillus sp. CM96 in this study. This pectinase’s activity can reach 17,000 U·g−1 after fermentation with a tray bioreactor, an increase of 86% compared to that obtained using flask liquid fermentation. The pectinase was purified and its characteristics were explored. Additionally, during pectinase fermentation, the activities of protease, glucanase, and cellulase were also determined to reach 7000, 8000, and 3000 U·g−1. The enzyme mixture was used to improve substrate digestion efficiency in 144 Soviet white pigs after adding a 0.05% cocktail enzyme for 38 days. The results showed that the average daily gain (ADG) increased by 139.41 ± 1.04 g·day−1, while the average daily feed intake (ADFI) and the feed conversion rate (FCR) decreased by 19.82 ± 1.64 g·day−1 and 0.07 ± 0.01 in 38 days, which indicated that the addition of hyperactive pectinase from the strain CM96 can increase nutrient digestibility and improve feed efficiency.
doi_str_mv 10.3390/pr12030615
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_3003410667</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3003410667</sourcerecordid><originalsourceid>FETCH-LOGICAL-c254t-31dc4987e8486f3bf60fdc1a8c9273fc8f95fee4ef8bc76573e2e6e743de9d983</originalsourceid><addsrcrecordid>eNpNkEtLAzEcxIMoWGovfoKAN2FrHrt5HEuxtlAfsHrxsqTZf3TLdrMmaaHf3pUKOpeZw48ZGISuKZlyrsldHygjnAhanKERY0xmWlJ5_i9fokmMWzJIU64KMULvpW-bOiuTSYAXEHbQDbHxHfYOL489BGNTcwD8AoN3JgLeHHH6BPzkD9DiMgXTdHgWB_Kjadt9xLGf4vmjFlfowpk2wuTXx-htcf86X2br54fVfLbOLCvylHFa21wrCSpXwvGNE8TVlhplNZPcWeV04QBycGpjpSgkBwYCZM5r0LVWfIxuTr198F97iKna-n3ohsmKE8JzSoSQA3V7omzwMQZwVR-anQnHipLq577q7z7-DdAiYdw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3003410667</pqid></control><display><type>article</type><title>Solid-State Fermentation of Hyperactive Pectinase by the Novel Strain Aspergillus sp. CM96</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><creator>Chen, Huiling ; Wan, Meimei ; Liu, Yang ; Yang, Guanghua ; Cai, Zhiqiang</creator><creatorcontrib>Chen, Huiling ; Wan, Meimei ; Liu, Yang ; Yang, Guanghua ; Cai, Zhiqiang</creatorcontrib><description>Pectinase, a kind of hydrolase, mainly contains polygalacturonase, pectinase, and pectin lyase, which can hydrolyze pectin to generate galacturonide and is widely used in industry. At present, pectinase’s activity is still relatively low. Hyperactive pectinase was produced with solid-phase fermentation and a tray bioreactor using the novel strain Aspergillus sp. CM96 in this study. This pectinase’s activity can reach 17,000 U·g−1 after fermentation with a tray bioreactor, an increase of 86% compared to that obtained using flask liquid fermentation. The pectinase was purified and its characteristics were explored. Additionally, during pectinase fermentation, the activities of protease, glucanase, and cellulase were also determined to reach 7000, 8000, and 3000 U·g−1. The enzyme mixture was used to improve substrate digestion efficiency in 144 Soviet white pigs after adding a 0.05% cocktail enzyme for 38 days. The results showed that the average daily gain (ADG) increased by 139.41 ± 1.04 g·day−1, while the average daily feed intake (ADFI) and the feed conversion rate (FCR) decreased by 19.82 ± 1.64 g·day−1 and 0.07 ± 0.01 in 38 days, which indicated that the addition of hyperactive pectinase from the strain CM96 can increase nutrient digestibility and improve feed efficiency.</description><identifier>ISSN: 2227-9717</identifier><identifier>EISSN: 2227-9717</identifier><identifier>DOI: 10.3390/pr12030615</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Aspergillus ; Bioreactors ; Carbon ; Cellulase ; Chemical bonds ; Digestibility ; Enzymes ; Feed efficiency ; Feeds ; Fermentation ; Livestock ; Nitrogen ; Pectin ; Pectin lyase ; Pectinase ; Polygalacturonase ; Poultry ; Proteins ; Solid phases ; Solid state fermentation ; Soybeans ; Substrates ; Temperature</subject><ispartof>Processes, 2024-03, Vol.12 (3), p.615</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c254t-31dc4987e8486f3bf60fdc1a8c9273fc8f95fee4ef8bc76573e2e6e743de9d983</cites><orcidid>0000-0002-9180-675X ; 0000-0002-0987-3671</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Chen, Huiling</creatorcontrib><creatorcontrib>Wan, Meimei</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Yang, Guanghua</creatorcontrib><creatorcontrib>Cai, Zhiqiang</creatorcontrib><title>Solid-State Fermentation of Hyperactive Pectinase by the Novel Strain Aspergillus sp. CM96</title><title>Processes</title><description>Pectinase, a kind of hydrolase, mainly contains polygalacturonase, pectinase, and pectin lyase, which can hydrolyze pectin to generate galacturonide and is widely used in industry. At present, pectinase’s activity is still relatively low. Hyperactive pectinase was produced with solid-phase fermentation and a tray bioreactor using the novel strain Aspergillus sp. CM96 in this study. This pectinase’s activity can reach 17,000 U·g−1 after fermentation with a tray bioreactor, an increase of 86% compared to that obtained using flask liquid fermentation. The pectinase was purified and its characteristics were explored. Additionally, during pectinase fermentation, the activities of protease, glucanase, and cellulase were also determined to reach 7000, 8000, and 3000 U·g−1. The enzyme mixture was used to improve substrate digestion efficiency in 144 Soviet white pigs after adding a 0.05% cocktail enzyme for 38 days. The results showed that the average daily gain (ADG) increased by 139.41 ± 1.04 g·day−1, while the average daily feed intake (ADFI) and the feed conversion rate (FCR) decreased by 19.82 ± 1.64 g·day−1 and 0.07 ± 0.01 in 38 days, which indicated that the addition of hyperactive pectinase from the strain CM96 can increase nutrient digestibility and improve feed efficiency.</description><subject>Aspergillus</subject><subject>Bioreactors</subject><subject>Carbon</subject><subject>Cellulase</subject><subject>Chemical bonds</subject><subject>Digestibility</subject><subject>Enzymes</subject><subject>Feed efficiency</subject><subject>Feeds</subject><subject>Fermentation</subject><subject>Livestock</subject><subject>Nitrogen</subject><subject>Pectin</subject><subject>Pectin lyase</subject><subject>Pectinase</subject><subject>Polygalacturonase</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Solid phases</subject><subject>Solid state fermentation</subject><subject>Soybeans</subject><subject>Substrates</subject><subject>Temperature</subject><issn>2227-9717</issn><issn>2227-9717</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNkEtLAzEcxIMoWGovfoKAN2FrHrt5HEuxtlAfsHrxsqTZf3TLdrMmaaHf3pUKOpeZw48ZGISuKZlyrsldHygjnAhanKERY0xmWlJ5_i9fokmMWzJIU64KMULvpW-bOiuTSYAXEHbQDbHxHfYOL489BGNTcwD8AoN3JgLeHHH6BPzkD9DiMgXTdHgWB_Kjadt9xLGf4vmjFlfowpk2wuTXx-htcf86X2br54fVfLbOLCvylHFa21wrCSpXwvGNE8TVlhplNZPcWeV04QBycGpjpSgkBwYCZM5r0LVWfIxuTr198F97iKna-n3ohsmKE8JzSoSQA3V7omzwMQZwVR-anQnHipLq577q7z7-DdAiYdw</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Chen, Huiling</creator><creator>Wan, Meimei</creator><creator>Liu, Yang</creator><creator>Yang, Guanghua</creator><creator>Cai, Zhiqiang</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>LK8</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0002-9180-675X</orcidid><orcidid>https://orcid.org/0000-0002-0987-3671</orcidid></search><sort><creationdate>20240301</creationdate><title>Solid-State Fermentation of Hyperactive Pectinase by the Novel Strain Aspergillus sp. CM96</title><author>Chen, Huiling ; Wan, Meimei ; Liu, Yang ; Yang, Guanghua ; Cai, Zhiqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c254t-31dc4987e8486f3bf60fdc1a8c9273fc8f95fee4ef8bc76573e2e6e743de9d983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aspergillus</topic><topic>Bioreactors</topic><topic>Carbon</topic><topic>Cellulase</topic><topic>Chemical bonds</topic><topic>Digestibility</topic><topic>Enzymes</topic><topic>Feed efficiency</topic><topic>Feeds</topic><topic>Fermentation</topic><topic>Livestock</topic><topic>Nitrogen</topic><topic>Pectin</topic><topic>Pectin lyase</topic><topic>Pectinase</topic><topic>Polygalacturonase</topic><topic>Poultry</topic><topic>Proteins</topic><topic>Solid phases</topic><topic>Solid state fermentation</topic><topic>Soybeans</topic><topic>Substrates</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Huiling</creatorcontrib><creatorcontrib>Wan, Meimei</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Yang, Guanghua</creatorcontrib><creatorcontrib>Cai, Zhiqiang</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Processes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Huiling</au><au>Wan, Meimei</au><au>Liu, Yang</au><au>Yang, Guanghua</au><au>Cai, Zhiqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Solid-State Fermentation of Hyperactive Pectinase by the Novel Strain Aspergillus sp. CM96</atitle><jtitle>Processes</jtitle><date>2024-03-01</date><risdate>2024</risdate><volume>12</volume><issue>3</issue><spage>615</spage><pages>615-</pages><issn>2227-9717</issn><eissn>2227-9717</eissn><abstract>Pectinase, a kind of hydrolase, mainly contains polygalacturonase, pectinase, and pectin lyase, which can hydrolyze pectin to generate galacturonide and is widely used in industry. At present, pectinase’s activity is still relatively low. Hyperactive pectinase was produced with solid-phase fermentation and a tray bioreactor using the novel strain Aspergillus sp. CM96 in this study. This pectinase’s activity can reach 17,000 U·g−1 after fermentation with a tray bioreactor, an increase of 86% compared to that obtained using flask liquid fermentation. The pectinase was purified and its characteristics were explored. Additionally, during pectinase fermentation, the activities of protease, glucanase, and cellulase were also determined to reach 7000, 8000, and 3000 U·g−1. The enzyme mixture was used to improve substrate digestion efficiency in 144 Soviet white pigs after adding a 0.05% cocktail enzyme for 38 days. The results showed that the average daily gain (ADG) increased by 139.41 ± 1.04 g·day−1, while the average daily feed intake (ADFI) and the feed conversion rate (FCR) decreased by 19.82 ± 1.64 g·day−1 and 0.07 ± 0.01 in 38 days, which indicated that the addition of hyperactive pectinase from the strain CM96 can increase nutrient digestibility and improve feed efficiency.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/pr12030615</doi><orcidid>https://orcid.org/0000-0002-9180-675X</orcidid><orcidid>https://orcid.org/0000-0002-0987-3671</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2227-9717
ispartof Processes, 2024-03, Vol.12 (3), p.615
issn 2227-9717
2227-9717
language eng
recordid cdi_proquest_journals_3003410667
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute
subjects Aspergillus
Bioreactors
Carbon
Cellulase
Chemical bonds
Digestibility
Enzymes
Feed efficiency
Feeds
Fermentation
Livestock
Nitrogen
Pectin
Pectin lyase
Pectinase
Polygalacturonase
Poultry
Proteins
Solid phases
Solid state fermentation
Soybeans
Substrates
Temperature
title Solid-State Fermentation of Hyperactive Pectinase by the Novel Strain Aspergillus sp. CM96
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T16%3A52%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Solid-State%20Fermentation%20of%20Hyperactive%20Pectinase%20by%20the%20Novel%20Strain%20Aspergillus%20sp.%20CM96&rft.jtitle=Processes&rft.au=Chen,%20Huiling&rft.date=2024-03-01&rft.volume=12&rft.issue=3&rft.spage=615&rft.pages=615-&rft.issn=2227-9717&rft.eissn=2227-9717&rft_id=info:doi/10.3390/pr12030615&rft_dat=%3Cproquest_cross%3E3003410667%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3003410667&rft_id=info:pmid/&rfr_iscdi=true