Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant
Palm Oil Mill Effluent (POME) is a liquid palm oil waste that has beneficial value because of the nutritional content it contains. This research aim to obtain biosurfactant by using POME as a growth substrate. The methods used include biosurfactant production, extraction using acid precipitation, an...
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| Veröffentlicht in: | IOP conference series. Earth and environmental science 2024-02, Vol.1312 (1), p.12057 |
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| creator | Damayana, H Nurhasanah Kiswandono, A A Juliasih, N L G R Bahri, S |
| description | Palm Oil Mill Effluent (POME) is a liquid palm oil waste that has beneficial value because of the nutritional content it contains. This research aim to obtain biosurfactant by using POME as a growth substrate. The methods used include biosurfactant production, extraction using acid precipitation, and characterization using TLC, FTIR, and LC-MS. The results showed that the local indigenous bacteria
Bacillus sp.
ALP D1 can produce biosurfactant under conditions of growth of POME 3%, NaNO
3
3%, pH 6, salinity 7% with an IE24 of 69.44%, diameter of oil spreading test at 8.5 cm, and positive at drop collapse test. The extraction stage produces a brownish-yellow crude extract of 0.068 g/L. Biosurfactant characterization using TLC showed pink spots with ninhydrin reagent indicating the presence of nitrogen groups from peptide in the biosurfactant. This is supported by the results of FTIR analysis with the presence of an N-H peak from the peptide group in the absorption area 3384 cm
-1
. The results of the LC-MS analysis showed that the biosurfactant obtained had Rt 14.64 minutes and 332.22 m/z. Based on the results, it is suspected that the biosurfactant produced by
Bacillus sp.
ALP D1 is a lipopeptide. |
| doi_str_mv | 10.1088/1755-1315/1312/1/012057 |
| format | Article |
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Bacillus sp.
ALP D1 can produce biosurfactant under conditions of growth of POME 3%, NaNO
3
3%, pH 6, salinity 7% with an IE24 of 69.44%, diameter of oil spreading test at 8.5 cm, and positive at drop collapse test. The extraction stage produces a brownish-yellow crude extract of 0.068 g/L. Biosurfactant characterization using TLC showed pink spots with ninhydrin reagent indicating the presence of nitrogen groups from peptide in the biosurfactant. This is supported by the results of FTIR analysis with the presence of an N-H peak from the peptide group in the absorption area 3384 cm
-1
. The results of the LC-MS analysis showed that the biosurfactant obtained had Rt 14.64 minutes and 332.22 m/z. Based on the results, it is suspected that the biosurfactant produced by
Bacillus sp.
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Bacillus sp.
ALP D1 can produce biosurfactant under conditions of growth of POME 3%, NaNO
3
3%, pH 6, salinity 7% with an IE24 of 69.44%, diameter of oil spreading test at 8.5 cm, and positive at drop collapse test. The extraction stage produces a brownish-yellow crude extract of 0.068 g/L. Biosurfactant characterization using TLC showed pink spots with ninhydrin reagent indicating the presence of nitrogen groups from peptide in the biosurfactant. This is supported by the results of FTIR analysis with the presence of an N-H peak from the peptide group in the absorption area 3384 cm
-1
. The results of the LC-MS analysis showed that the biosurfactant obtained had Rt 14.64 minutes and 332.22 m/z. Based on the results, it is suspected that the biosurfactant produced by
Bacillus sp.
ALP D1 is a lipopeptide.</description><subject>Bacillus</subject><subject>Bacillus sp</subject><subject>Bacteria</subject><subject>biosurfactant</subject><subject>lipopeptide</subject><subject>Ninhydrin</subject><subject>Oil wastes</subject><subject>Palm oil</subject><subject>Peptides</subject><subject>POME</subject><subject>Production methods</subject><subject>Reagents</subject><subject>Substrates</subject><subject>Surfactants</subject><issn>1755-1307</issn><issn>1755-1315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>O3W</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkF9LwzAUxYMoOKefwYBPPmxNmnZpH-esf2Cyge45JE0yM2rTJS2in96UykQQfLkJN-ece_MD4BKjKUZZFmGaphNMcBqFEkc4QjhGKT0Co8PL8eGO6Ck4836H0IwmJB-B_aY1lfnkrbE1XK-eCsg93Dr73r5C3wnfOt4qqK2DlS15BU0tzVbVtvNQ8LJVznB4w0tTVaHjmymcL9fwFgcdbJyVXWnqLRTG-s7poOd1ew5ONK-8uvg-x2BzV7wsHibL1f3jYr6clDEldCKlIrkWmZ5xobHESsxknidEpAlWJRVYZkn4g8RprEmiEqJykcU0y2jOcyUlGYOrITfsse-Ub9nOdq4OI1mcpzQMIQHBGNBBVTrrvVOaNc68cffBMGI9X9aTYz3FvsQMs4FvcJLBaWzzE_2_6_oPV1E8_9axRmryBQ7jip8</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Damayana, H</creator><creator>Nurhasanah</creator><creator>Kiswandono, A A</creator><creator>Juliasih, N L G R</creator><creator>Bahri, S</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope></search><sort><creationdate>20240201</creationdate><title>Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant</title><author>Damayana, H ; Nurhasanah ; Kiswandono, A A ; Juliasih, N L G R ; Bahri, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2737-dde39fb8f6abf1d1eb6d9943b541ec7b1d84674d152f34e43e9b8278879a9edd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bacillus</topic><topic>Bacillus sp</topic><topic>Bacteria</topic><topic>biosurfactant</topic><topic>lipopeptide</topic><topic>Ninhydrin</topic><topic>Oil wastes</topic><topic>Palm oil</topic><topic>Peptides</topic><topic>POME</topic><topic>Production methods</topic><topic>Reagents</topic><topic>Substrates</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Damayana, H</creatorcontrib><creatorcontrib>Nurhasanah</creatorcontrib><creatorcontrib>Kiswandono, A A</creatorcontrib><creatorcontrib>Juliasih, N L G R</creatorcontrib><creatorcontrib>Bahri, S</creatorcontrib><collection>IOP Publishing Free Content</collection><collection>IOPscience (Open Access)</collection><collection>CrossRef</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><jtitle>IOP conference series. Earth and environmental science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Damayana, H</au><au>Nurhasanah</au><au>Kiswandono, A A</au><au>Juliasih, N L G R</au><au>Bahri, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant</atitle><jtitle>IOP conference series. Earth and environmental science</jtitle><addtitle>IOP Conf. Ser.: Earth Environ. Sci</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>1312</volume><issue>1</issue><spage>12057</spage><pages>12057-</pages><issn>1755-1307</issn><eissn>1755-1315</eissn><abstract>Palm Oil Mill Effluent (POME) is a liquid palm oil waste that has beneficial value because of the nutritional content it contains. This research aim to obtain biosurfactant by using POME as a growth substrate. The methods used include biosurfactant production, extraction using acid precipitation, and characterization using TLC, FTIR, and LC-MS. The results showed that the local indigenous bacteria
Bacillus sp.
ALP D1 can produce biosurfactant under conditions of growth of POME 3%, NaNO
3
3%, pH 6, salinity 7% with an IE24 of 69.44%, diameter of oil spreading test at 8.5 cm, and positive at drop collapse test. The extraction stage produces a brownish-yellow crude extract of 0.068 g/L. Biosurfactant characterization using TLC showed pink spots with ninhydrin reagent indicating the presence of nitrogen groups from peptide in the biosurfactant. This is supported by the results of FTIR analysis with the presence of an N-H peak from the peptide group in the absorption area 3384 cm
-1
. The results of the LC-MS analysis showed that the biosurfactant obtained had Rt 14.64 minutes and 332.22 m/z. Based on the results, it is suspected that the biosurfactant produced by
Bacillus sp.
ALP D1 is a lipopeptide.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1755-1315/1312/1/012057</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus Bacillus sp Bacteria biosurfactant lipopeptide Ninhydrin Oil wastes Palm oil Peptides POME Production methods Reagents Substrates Surfactants |
| title | Utilization POME as growth substrate for local indigenous bacteria Bacillus sp. ALP D1 in producing biosurfactant |
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