A novel molecular imprinted electrochemical biosensor for sensitive determination of Ganoderic acid A

Analytical approaches with the features of easy manipulation, cost-effectiveness, and miniature are needed for practice-oriented point-of-care diagnostics. This work prepared the molecularly imprinted polymers (MIPs) to selectively detect Ganoderic acid A (GAA). To determine the optimum composition, we examined diverse functional monomers through batch rebinding experiments. The new method uses nanomaterials-functionalized screen-printed carbon electrode (SPCE) to selectively measure GAA, the critical ingredient in Ganoderma lucidum ( G. lucidum ). Reduced graphene oxide (RGO) chitosan attached to SPCEs has offered the electrochemical sensor core to detect GAA. Cyclic voltammetry (CV) was performed on the SPCE to electropolymerize MIP using a solution containing GAA with o-phenylenediamine being the template. Thereafter, this sensor was characterized by scanning electron microscopy (SEM), and CV together with electrochemical impedance spectroscopy (EIS). GAA was analyzed through differential pulse voltammetry (DPV) within the 0.1-100 ng/mL linear range, and the limit of detection (LOD) was 0.03 ng/mL. The sensor’s simplicity, selectivity, and environmental friendliness make it ideal to detect GAA.