Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment
Hydrogen (H 2 ) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed, Eichhornia crassipes, using facultative anaerobic bacteria. A total of 12 bacterial strains w...
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| creator | Mechery, Jerry Biji, B. Thomas, Daniya M. Sylas, V. P. |
| description | Hydrogen (H
2
) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed,
Eichhornia crassipes,
using facultative anaerobic bacteria. A total of 12 bacterial strains were isolated from different wastewater sources and were screened for the potential of H
2
production using glucose as carbon source. Ten strains showed the H
2
-producing potential and were identified up to the generic level by biochemical tests. Two strains with higher H
2
production were sequenced using PCR technique and identified as
Proteus mirabilis
and
Pseudomonas aeruginosa
and selected for the studies with
E. crassipes
as the substrate. It was found that
P. aeruginosa
could produce 19.54 ± 0.03% of H
2
from 2% acid (H
2
SO
4
) treated substrate which was comparatively higher than that of 4 and 8% treatments.
P. mirabilis
also yielded better results of 5.42 ± 0.02% H
2
f or 2% acid (H
2
SO
4
) treated substrate than 4 and 8% treatments. In total, 33.52 ± 0.04% of H
2
was produced by
P. aeruginosa
for the substrate treated with 2% alkali (NaOH). It was noted that with respect to
P. mirabilis
4% alkali treated substrate yielded a higher percentage of H
2
(20.23 ± 0.03%) compared to the other two concentrations. The results indicate that alkali treated substrate produced comparatively higher amount of H
2
than that of acid treated substrates. Regarding efficiency,
P. aeruginosa
was found to be more competent than
P. mirabilis. |
| doi_str_mv | 10.1007/s40974-017-0069-4 |
| format | Article |
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2
) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed,
Eichhornia crassipes,
using facultative anaerobic bacteria. A total of 12 bacterial strains were isolated from different wastewater sources and were screened for the potential of H
2
production using glucose as carbon source. Ten strains showed the H
2
-producing potential and were identified up to the generic level by biochemical tests. Two strains with higher H
2
production were sequenced using PCR technique and identified as
Proteus mirabilis
and
Pseudomonas aeruginosa
and selected for the studies with
E. crassipes
as the substrate. It was found that
P. aeruginosa
could produce 19.54 ± 0.03% of H
2
from 2% acid (H
2
SO
4
) treated substrate which was comparatively higher than that of 4 and 8% treatments.
P. mirabilis
also yielded better results of 5.42 ± 0.02% H
2
f or 2% acid (H
2
SO
4
) treated substrate than 4 and 8% treatments. In total, 33.52 ± 0.04% of H
2
was produced by
P. aeruginosa
for the substrate treated with 2% alkali (NaOH). It was noted that with respect to
P. mirabilis
4% alkali treated substrate yielded a higher percentage of H
2
(20.23 ± 0.03%) compared to the other two concentrations. The results indicate that alkali treated substrate produced comparatively higher amount of H
2
than that of acid treated substrates. Regarding efficiency,
P. aeruginosa
was found to be more competent than
P. mirabilis.</description><identifier>ISSN: 2363-7692</identifier><identifier>EISSN: 2363-8338</identifier><identifier>DOI: 10.1007/s40974-017-0069-4</identifier><language>eng</language><publisher>College Park, MD: Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University</publisher><subject>Acids ; Anaerobic bacteria ; Aquatic plants ; Aquatic weeds ; Bacteria ; Biochemical tests ; Biohydrogen ; Biomass ; Carbon sources ; Clean energy ; Climate change ; Ecology ; Eichhornia crassipes ; Energy ; Environment ; Experiments ; Fermentation ; Fossil fuels ; Glucose ; Hydrogen ; Hydrogen production ; Original Article ; Proteus mirabilis ; Pseudomonas aeruginosa ; Salmonella ; Sodium hydroxide ; Strains (organisms) ; Substrates ; Sulfuric acid</subject><ispartof>Energy, ecology and environment (Online), 2017-10, Vol.2 (5), p.350-359</ispartof><rights>Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University and Springer-Verlag GmbH Germany 2017</rights><rights>Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University and Springer-Verlag GmbH Germany 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-51023d0c1bc4061463f0352c4cbee2860a328ffee145e2030dbfef94d219a0253</citedby><cites>FETCH-LOGICAL-c396t-51023d0c1bc4061463f0352c4cbee2860a328ffee145e2030dbfef94d219a0253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40974-017-0069-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2938251701?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,41464,42533,43781,51294</link.rule.ids></links><search><creatorcontrib>Mechery, Jerry</creatorcontrib><creatorcontrib>Biji, B.</creatorcontrib><creatorcontrib>Thomas, Daniya M.</creatorcontrib><creatorcontrib>Sylas, V. P.</creatorcontrib><title>Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment</title><title>Energy, ecology and environment (Online)</title><addtitle>Energ. Ecol. Environ</addtitle><description>Hydrogen (H
2
) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed,
Eichhornia crassipes,
using facultative anaerobic bacteria. A total of 12 bacterial strains were isolated from different wastewater sources and were screened for the potential of H
2
production using glucose as carbon source. Ten strains showed the H
2
-producing potential and were identified up to the generic level by biochemical tests. Two strains with higher H
2
production were sequenced using PCR technique and identified as
Proteus mirabilis
and
Pseudomonas aeruginosa
and selected for the studies with
E. crassipes
as the substrate. It was found that
P. aeruginosa
could produce 19.54 ± 0.03% of H
2
from 2% acid (H
2
SO
4
) treated substrate which was comparatively higher than that of 4 and 8% treatments.
P. mirabilis
also yielded better results of 5.42 ± 0.02% H
2
f or 2% acid (H
2
SO
4
) treated substrate than 4 and 8% treatments. In total, 33.52 ± 0.04% of H
2
was produced by
P. aeruginosa
for the substrate treated with 2% alkali (NaOH). It was noted that with respect to
P. mirabilis
4% alkali treated substrate yielded a higher percentage of H
2
(20.23 ± 0.03%) compared to the other two concentrations. The results indicate that alkali treated substrate produced comparatively higher amount of H
2
than that of acid treated substrates. Regarding efficiency,
P. aeruginosa
was found to be more competent than
P. mirabilis.</description><subject>Acids</subject><subject>Anaerobic bacteria</subject><subject>Aquatic plants</subject><subject>Aquatic weeds</subject><subject>Bacteria</subject><subject>Biochemical tests</subject><subject>Biohydrogen</subject><subject>Biomass</subject><subject>Carbon sources</subject><subject>Clean energy</subject><subject>Climate change</subject><subject>Ecology</subject><subject>Eichhornia crassipes</subject><subject>Energy</subject><subject>Environment</subject><subject>Experiments</subject><subject>Fermentation</subject><subject>Fossil fuels</subject><subject>Glucose</subject><subject>Hydrogen</subject><subject>Hydrogen production</subject><subject>Original Article</subject><subject>Proteus mirabilis</subject><subject>Pseudomonas aeruginosa</subject><subject>Salmonella</subject><subject>Sodium hydroxide</subject><subject>Strains (organisms)</subject><subject>Substrates</subject><subject>Sulfuric acid</subject><issn>2363-7692</issn><issn>2363-8338</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1UUtLAzEQXkTBov4AbwHPq5PHvrxpqQ8QvOg5ZLOTNppuapIV-kv8u6a04MnDMMPM92D4iuKSwjUFaG6igK4RJdCmBKi7UhwVM8ZrXract8eHuak7dlpcxGh7EILRtmvaWfFzb_1qOwS_xJFsgh8mnawfSb8lzmvl3JbY6J1KOBCj9OSSSvYbSa90wmCVI3GD2mIkU7TjkqRVvlm_VjESb8jC6tXKh9EqokPe2Q3GW4LGoE67u9J2IGrM5T6VsyQFVGmNYzovToxyES8O_ax4f1i8zZ_Kl9fH5_ndS6l5V6eyosD4AJr2WkBNRc0N8IppoXtE1tagOGuzG1JRIQMOQ2_QdGJgtFPAKn5WXO118-9fE8YkP_wUxmwpWcdbVtEGaEbRPUoHH2NAIzfBrlXYSgpyF4HcRyBzBHIXgRSZw_acmLHjEsOf8v-kX0zljCE</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Mechery, Jerry</creator><creator>Biji, B.</creator><creator>Thomas, Daniya M.</creator><creator>Sylas, V. P.</creator><general>Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope></search><sort><creationdate>20171001</creationdate><title>Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment</title><author>Mechery, Jerry ; Biji, B. ; Thomas, Daniya M. ; Sylas, V. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-51023d0c1bc4061463f0352c4cbee2860a328ffee145e2030dbfef94d219a0253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acids</topic><topic>Anaerobic bacteria</topic><topic>Aquatic plants</topic><topic>Aquatic weeds</topic><topic>Bacteria</topic><topic>Biochemical tests</topic><topic>Biohydrogen</topic><topic>Biomass</topic><topic>Carbon sources</topic><topic>Clean energy</topic><topic>Climate change</topic><topic>Ecology</topic><topic>Eichhornia crassipes</topic><topic>Energy</topic><topic>Environment</topic><topic>Experiments</topic><topic>Fermentation</topic><topic>Fossil fuels</topic><topic>Glucose</topic><topic>Hydrogen</topic><topic>Hydrogen production</topic><topic>Original Article</topic><topic>Proteus mirabilis</topic><topic>Pseudomonas aeruginosa</topic><topic>Salmonella</topic><topic>Sodium hydroxide</topic><topic>Strains (organisms)</topic><topic>Substrates</topic><topic>Sulfuric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mechery, Jerry</creatorcontrib><creatorcontrib>Biji, B.</creatorcontrib><creatorcontrib>Thomas, Daniya M.</creatorcontrib><creatorcontrib>Sylas, V. 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P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment</atitle><jtitle>Energy, ecology and environment (Online)</jtitle><stitle>Energ. Ecol. Environ</stitle><date>2017-10-01</date><risdate>2017</risdate><volume>2</volume><issue>5</issue><spage>350</spage><epage>359</epage><pages>350-359</pages><issn>2363-7692</issn><eissn>2363-8338</eissn><abstract>Hydrogen (H
2
) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed,
Eichhornia crassipes,
using facultative anaerobic bacteria. A total of 12 bacterial strains were isolated from different wastewater sources and were screened for the potential of H
2
production using glucose as carbon source. Ten strains showed the H
2
-producing potential and were identified up to the generic level by biochemical tests. Two strains with higher H
2
production were sequenced using PCR technique and identified as
Proteus mirabilis
and
Pseudomonas aeruginosa
and selected for the studies with
E. crassipes
as the substrate. It was found that
P. aeruginosa
could produce 19.54 ± 0.03% of H
2
from 2% acid (H
2
SO
4
) treated substrate which was comparatively higher than that of 4 and 8% treatments.
P. mirabilis
also yielded better results of 5.42 ± 0.02% H
2
f or 2% acid (H
2
SO
4
) treated substrate than 4 and 8% treatments. In total, 33.52 ± 0.04% of H
2
was produced by
P. aeruginosa
for the substrate treated with 2% alkali (NaOH). It was noted that with respect to
P. mirabilis
4% alkali treated substrate yielded a higher percentage of H
2
(20.23 ± 0.03%) compared to the other two concentrations. The results indicate that alkali treated substrate produced comparatively higher amount of H
2
than that of acid treated substrates. Regarding efficiency,
P. aeruginosa
was found to be more competent than
P. mirabilis.</abstract><cop>College Park, MD</cop><pub>Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University</pub><doi>10.1007/s40974-017-0069-4</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2363-7692 |
| ispartof | Energy, ecology and environment (Online), 2017-10, Vol.2 (5), p.350-359 |
| issn | 2363-7692 2363-8338 |
| language | eng |
| recordid | cdi_proquest_journals_2938251701 |
| source | SpringerLink Journals; ProQuest Central |
| subjects | Acids Anaerobic bacteria Aquatic plants Aquatic weeds Bacteria Biochemical tests Biohydrogen Biomass Carbon sources Clean energy Climate change Ecology Eichhornia crassipes Energy Environment Experiments Fermentation Fossil fuels Glucose Hydrogen Hydrogen production Original Article Proteus mirabilis Pseudomonas aeruginosa Salmonella Sodium hydroxide Strains (organisms) Substrates Sulfuric acid |
| title | Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment |
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