Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco
NADPH-dependent thioredoxin reductase (NTR), which is a flavin protein family of disulfide oxidoreductases present in all living cells, reduces thioredoxin (Trx). The roles of NTR B under stress conditions have not been well characterized. In this study, a tomato NTR B (SlNTRB) gene was cloned and f...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2024-03, Vol.156 (3), p.94, Article 94 |
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| creator | Nie, Yuting Qiao, Shengtai Li, Kunzhi Xu, Huini |
| description | NADPH-dependent thioredoxin reductase (NTR), which is a flavin protein family of disulfide oxidoreductases present in all living cells, reduces thioredoxin (Trx). The roles of NTR B under stress conditions have not been well characterized. In this study, a tomato NTR B (SlNTRB) gene was cloned and functionally characterized. The mRNA transcript level of
SlNTRB
was induced significantly after nitrate treatment. The seed germination rate of
SlNTRB
overexpressed transgenic tobacco was higher than wild type (WT) under nitrate stress. The growth of
SlNTRB
overexpressed plants was better than WT and the reactive oxygen species (ROS) accumulation were lower than WT under nitrate stress. The NTR activity, superoxide dismutase (SOD) activity, the GSH/GSSG ratio, and the expression of
Trx
were significantly higher than WT after nitrate stress treatment. The
SlNTRB
overexpressed transgenic tobacco seeds showed higher tolerance to H
2
O
2
and methyl viologen (MV) treatments than WT. The above results indicated that the
SlNTRB
gene improved the plant nitrate stress tolerance by alleviating the oxidative damage.
Key message
Overexpression of tomato
SlNTRB
gene in tobacco enhances the excess nitrate stress tolerance with less oxidative damage. |
| doi_str_mv | 10.1007/s11240-023-02661-w |
| format | Article |
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SlNTRB
was induced significantly after nitrate treatment. The seed germination rate of
SlNTRB
overexpressed transgenic tobacco was higher than wild type (WT) under nitrate stress. The growth of
SlNTRB
overexpressed plants was better than WT and the reactive oxygen species (ROS) accumulation were lower than WT under nitrate stress. The NTR activity, superoxide dismutase (SOD) activity, the GSH/GSSG ratio, and the expression of
Trx
were significantly higher than WT after nitrate stress treatment. The
SlNTRB
overexpressed transgenic tobacco seeds showed higher tolerance to H
2
O
2
and methyl viologen (MV) treatments than WT. The above results indicated that the
SlNTRB
gene improved the plant nitrate stress tolerance by alleviating the oxidative damage.
Key message
Overexpression of tomato
SlNTRB
gene in tobacco enhances the excess nitrate stress tolerance with less oxidative damage.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-023-02661-w</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Damage tolerance ; Flavin ; Germination ; Hydrogen peroxide ; Life Sciences ; Methyl viologen ; Nitrates ; Original Article ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Reactive oxygen species ; Reductases ; Seed germination ; Seeds ; Superoxide dismutase ; Thioredoxin ; Tobacco ; Tomatoes ; Transgenic plants</subject><ispartof>Plant cell, tissue and organ culture, 2024-03, Vol.156 (3), p.94, Article 94</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-7798e7bfa86dbd64f2089fdbf485af7f5d70688bb9156c0bbc19c5a24c0cd1c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-023-02661-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-023-02661-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Nie, Yuting</creatorcontrib><creatorcontrib>Qiao, Shengtai</creatorcontrib><creatorcontrib>Li, Kunzhi</creatorcontrib><creatorcontrib>Xu, Huini</creatorcontrib><title>Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>NADPH-dependent thioredoxin reductase (NTR), which is a flavin protein family of disulfide oxidoreductases present in all living cells, reduces thioredoxin (Trx). The roles of NTR B under stress conditions have not been well characterized. In this study, a tomato NTR B (SlNTRB) gene was cloned and functionally characterized. The mRNA transcript level of
SlNTRB
was induced significantly after nitrate treatment. The seed germination rate of
SlNTRB
overexpressed transgenic tobacco was higher than wild type (WT) under nitrate stress. The growth of
SlNTRB
overexpressed plants was better than WT and the reactive oxygen species (ROS) accumulation were lower than WT under nitrate stress. The NTR activity, superoxide dismutase (SOD) activity, the GSH/GSSG ratio, and the expression of
Trx
were significantly higher than WT after nitrate stress treatment. The
SlNTRB
overexpressed transgenic tobacco seeds showed higher tolerance to H
2
O
2
and methyl viologen (MV) treatments than WT. The above results indicated that the
SlNTRB
gene improved the plant nitrate stress tolerance by alleviating the oxidative damage.
Key message
Overexpression of tomato
SlNTRB
gene in tobacco enhances the excess nitrate stress tolerance with less oxidative damage.</description><subject>Biomedical and Life Sciences</subject><subject>Damage tolerance</subject><subject>Flavin</subject><subject>Germination</subject><subject>Hydrogen peroxide</subject><subject>Life Sciences</subject><subject>Methyl viologen</subject><subject>Nitrates</subject><subject>Original Article</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Reactive oxygen species</subject><subject>Reductases</subject><subject>Seed germination</subject><subject>Seeds</subject><subject>Superoxide dismutase</subject><subject>Thioredoxin</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Transgenic plants</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAUhYMoOI7-AVcB19WkTZN0qYMvGBR0XIc8a4c-xiTz-vdmrODOxeVy4Hzncg8AlxhdY4TYTcA4JyhDeZGGUpxtj8AEl6zISkTIMZggTFlGeclOwVkIS4QQLQiegNVi6GQc4Hv7sni7g0238sPGGmh32oYA-yZ6GS0M0R9kHFrrZa8tVHso29ZuGhmbvobx08Jh15ikNhYa2cnawqaHie5DbftGJ1ZJrYdzcOJkG-zF756Cj4f7xewpm78-Ps9u55nOGYoZYxW3TDnJqVGGEpcjXjmjHOGldMyVhiHKuVIVLqlGSmlc6VLmRCNtsK6KKbgac9NDX2sbolgOa9-nkyKvCoZJxQlOrnx0aT-E4K0TK9900u8FRuLQrBibFalZ8dOs2CaoGKGQzH1t_V_0P9Q3GJZ-uA</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Nie, Yuting</creator><creator>Qiao, Shengtai</creator><creator>Li, Kunzhi</creator><creator>Xu, Huini</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20240301</creationdate><title>Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco</title><author>Nie, Yuting ; Qiao, Shengtai ; Li, Kunzhi ; Xu, Huini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-7798e7bfa86dbd64f2089fdbf485af7f5d70688bb9156c0bbc19c5a24c0cd1c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomedical and Life Sciences</topic><topic>Damage tolerance</topic><topic>Flavin</topic><topic>Germination</topic><topic>Hydrogen peroxide</topic><topic>Life Sciences</topic><topic>Methyl viologen</topic><topic>Nitrates</topic><topic>Original Article</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Reactive oxygen species</topic><topic>Reductases</topic><topic>Seed germination</topic><topic>Seeds</topic><topic>Superoxide dismutase</topic><topic>Thioredoxin</topic><topic>Tobacco</topic><topic>Tomatoes</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nie, Yuting</creatorcontrib><creatorcontrib>Qiao, Shengtai</creatorcontrib><creatorcontrib>Li, Kunzhi</creatorcontrib><creatorcontrib>Xu, Huini</creatorcontrib><collection>CrossRef</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nie, Yuting</au><au>Qiao, Shengtai</au><au>Li, Kunzhi</au><au>Xu, Huini</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2024-03-01</date><risdate>2024</risdate><volume>156</volume><issue>3</issue><spage>94</spage><pages>94-</pages><artnum>94</artnum><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>NADPH-dependent thioredoxin reductase (NTR), which is a flavin protein family of disulfide oxidoreductases present in all living cells, reduces thioredoxin (Trx). The roles of NTR B under stress conditions have not been well characterized. In this study, a tomato NTR B (SlNTRB) gene was cloned and functionally characterized. The mRNA transcript level of
SlNTRB
was induced significantly after nitrate treatment. The seed germination rate of
SlNTRB
overexpressed transgenic tobacco was higher than wild type (WT) under nitrate stress. The growth of
SlNTRB
overexpressed plants was better than WT and the reactive oxygen species (ROS) accumulation were lower than WT under nitrate stress. The NTR activity, superoxide dismutase (SOD) activity, the GSH/GSSG ratio, and the expression of
Trx
were significantly higher than WT after nitrate stress treatment. The
SlNTRB
overexpressed transgenic tobacco seeds showed higher tolerance to H
2
O
2
and methyl viologen (MV) treatments than WT. The above results indicated that the
SlNTRB
gene improved the plant nitrate stress tolerance by alleviating the oxidative damage.
Key message
Overexpression of tomato
SlNTRB
gene in tobacco enhances the excess nitrate stress tolerance with less oxidative damage.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-023-02661-w</doi></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2024-03, Vol.156 (3), p.94, Article 94 |
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| language | eng |
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| subjects | Biomedical and Life Sciences Damage tolerance Flavin Germination Hydrogen peroxide Life Sciences Methyl viologen Nitrates Original Article Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Reactive oxygen species Reductases Seed germination Seeds Superoxide dismutase Thioredoxin Tobacco Tomatoes Transgenic plants |
| title | Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco |
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