In vitro true root cultures of Lotus hebranicus Hochst. ex brand: profiling of secondary metabolites and plausible in vivo gastroprotective effect
In vitro, the Lotus hebranicus produces true root cultures under entirely growth regulator-free culture conditions. These roots are completely independent of the shoots and gives abundant amounts of biomass of true root culture as raw materials in a short time. Lately, gastric ulcer have gained an i...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2024-03, Vol.156 (3), p.93-93, Article 93 |
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| creator | Abdelazeez, Walla M. A. Maaty, Doaa A. M. El-Swaify, Zeinab A. Negm, Walaa A. Selim, Hend M. Aboueldis, Gamil R. |
| description | In vitro, the
Lotus hebranicus
produces true root cultures under entirely growth regulator-free culture conditions. These roots are completely independent of the shoots and gives abundant amounts of biomass of true root culture as raw materials in a short time. Lately, gastric ulcer have gained an increasing interest as it affects millions around the world. So, this study aimed to screen and evaluate the chemical constituents of the
L. hebranicus
extract (plant and true root culture), in addition to studying the biological effect of these extracts on mice with ethanol-induced gastric ulcer and the possible role of mediating oxidative stress, inflammation and TGFβ/Smad3 as underlying mechanisms. Fifty-four Albino mice were assigned into nine groups (n = 6). Lotus was orally administered for seven successive days, followed by a single dose of oral ethanol for ulcer induction. Using histological assessment and immunohistochemical studies as well as colorimetric analysis, ELISA and qRT-PCR, the results revealed that ethanol-induced ulcers exhibited a substantial increase (
p
|
| doi_str_mv | 10.1007/s11240-023-02672-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2937149476</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153628264</sourcerecordid><originalsourceid>FETCH-LOGICAL-c347t-771b7419899e3177e58cf23ecd68145e4ef8b142883533fb4c1b01f6e42cabd63</originalsourceid><addsrcrecordid>eNp9kcFqGzEQhkVpIK6TF8hJ0Esvm2okrbTbWwlNYjDkkp7FrjyyZdYrV9Ka5DX6xNHGhUIPPQwziO__Z9BPyA2wW2BMf00AXLKKcVFKaV7pD2QBtRZVzaT8SBYMlK5UU-tL8imlPWNMCQkL8ns10pPPMdAcJ6QxhEztNOQpYqLB0XXIU6I77GM3elvGx2B3Kd9SfKHz2-YbPcbg_ODH7cwntGHcdPGVHjB3fRh8LkaFo8ehm5LvB6R-XnkKdNulsrjIM9rsT0jRuTJdkQvXDQmv__Ql-Xn_4_nusVo_Pazuvq8rK6TOldbQawlt07YoQGusG-u4QLtRDcgaJbqmB8mbRtRCuF5a6Bk4hZLbrt8osSRfzr7lgl8TpmwOPlkchm7EMCUjoBaKN1zJgn7-B92HKY7lOsNboUG2Us-G_EzZGFKK6Mwx-kP5CwPMzDGZc0ymxGTeYzK6iMRZlAo8bjH-tf6P6g1f55eO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2937149476</pqid></control><display><type>article</type><title>In vitro true root cultures of Lotus hebranicus Hochst. ex brand: profiling of secondary metabolites and plausible in vivo gastroprotective effect</title><source>Springer Online Journals</source><creator>Abdelazeez, Walla M. A. ; Maaty, Doaa A. M. ; El-Swaify, Zeinab A. ; Negm, Walaa A. ; Selim, Hend M. ; Aboueldis, Gamil R.</creator><creatorcontrib>Abdelazeez, Walla M. A. ; Maaty, Doaa A. M. ; El-Swaify, Zeinab A. ; Negm, Walaa A. ; Selim, Hend M. ; Aboueldis, Gamil R.</creatorcontrib><description>In vitro, the
Lotus hebranicus
produces true root cultures under entirely growth regulator-free culture conditions. These roots are completely independent of the shoots and gives abundant amounts of biomass of true root culture as raw materials in a short time. Lately, gastric ulcer have gained an increasing interest as it affects millions around the world. So, this study aimed to screen and evaluate the chemical constituents of the
L. hebranicus
extract (plant and true root culture), in addition to studying the biological effect of these extracts on mice with ethanol-induced gastric ulcer and the possible role of mediating oxidative stress, inflammation and TGFβ/Smad3 as underlying mechanisms. Fifty-four Albino mice were assigned into nine groups (n = 6). Lotus was orally administered for seven successive days, followed by a single dose of oral ethanol for ulcer induction. Using histological assessment and immunohistochemical studies as well as colorimetric analysis, ELISA and qRT-PCR, the results revealed that ethanol-induced ulcers exhibited a substantial increase (
p
< 0.05) in malondialdehyde and interleukin 18 with a remarkable lessening in the gastro-protective mediators; superoxide dismutase, prostaglandin E2 and glutathione, parallel with an increase in TGFβ/Smad3 expressions relative to control. Pre-treatment with
L. hebranicus
showed a comparable result to those with omeprazole and showed a significant inhibitory effect on inflammatory mediators and significant elevation of the gastro-protective mediators relative to the positive control. In conclusion,
L. hebranicus
extract (plant/true root culture) has the potential to protect the gastric tissues against ethanol by inhibiting oxidative stress and inflammation and enhancing TGFβ/Smad3 mediators along with gastric protective mediators.
Key message
In vitro-grown true root cultures of
L. herbanicus
provide plant raw materials with potential application as a source of natural products against ethanol-induced gastric ulcer.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-023-02672-7</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>albino ; bioactive properties ; Biological effects ; biomass ; Biomedical and Life Sciences ; Colorimetry ; Enzyme-linked immunosorbent assay ; Ethanol ; Glutathione ; Growth regulators ; immunohistochemistry ; Inflammation ; Interleukin 18 ; Life Sciences ; Lotus ; malondialdehyde ; Metabolites ; Natural products ; Omeprazole ; Oral administration ; Original Article ; Oxidative stress ; Plant extracts ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Prostaglandin E2 ; prostaglandins ; Raw materials ; Secondary metabolites ; Smad3 protein ; stomach ulcers ; Superoxide dismutase ; Ulcers</subject><ispartof>Plant cell, tissue and organ culture, 2024-03, Vol.156 (3), p.93-93, Article 93</ispartof><rights>The Author(s) 2024</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c347t-771b7419899e3177e58cf23ecd68145e4ef8b142883533fb4c1b01f6e42cabd63</cites><orcidid>0000-0003-4971-2300 ; 0000-0002-0118-3967 ; 0000-0003-0463-8047 ; 0009-0000-5310-7571 ; 0000-0003-0868-696X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-023-02672-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-023-02672-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Abdelazeez, Walla M. A.</creatorcontrib><creatorcontrib>Maaty, Doaa A. M.</creatorcontrib><creatorcontrib>El-Swaify, Zeinab A.</creatorcontrib><creatorcontrib>Negm, Walaa A.</creatorcontrib><creatorcontrib>Selim, Hend M.</creatorcontrib><creatorcontrib>Aboueldis, Gamil R.</creatorcontrib><title>In vitro true root cultures of Lotus hebranicus Hochst. ex brand: profiling of secondary metabolites and plausible in vivo gastroprotective effect</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>In vitro, the
Lotus hebranicus
produces true root cultures under entirely growth regulator-free culture conditions. These roots are completely independent of the shoots and gives abundant amounts of biomass of true root culture as raw materials in a short time. Lately, gastric ulcer have gained an increasing interest as it affects millions around the world. So, this study aimed to screen and evaluate the chemical constituents of the
L. hebranicus
extract (plant and true root culture), in addition to studying the biological effect of these extracts on mice with ethanol-induced gastric ulcer and the possible role of mediating oxidative stress, inflammation and TGFβ/Smad3 as underlying mechanisms. Fifty-four Albino mice were assigned into nine groups (n = 6). Lotus was orally administered for seven successive days, followed by a single dose of oral ethanol for ulcer induction. Using histological assessment and immunohistochemical studies as well as colorimetric analysis, ELISA and qRT-PCR, the results revealed that ethanol-induced ulcers exhibited a substantial increase (
p
< 0.05) in malondialdehyde and interleukin 18 with a remarkable lessening in the gastro-protective mediators; superoxide dismutase, prostaglandin E2 and glutathione, parallel with an increase in TGFβ/Smad3 expressions relative to control. Pre-treatment with
L. hebranicus
showed a comparable result to those with omeprazole and showed a significant inhibitory effect on inflammatory mediators and significant elevation of the gastro-protective mediators relative to the positive control. In conclusion,
L. hebranicus
extract (plant/true root culture) has the potential to protect the gastric tissues against ethanol by inhibiting oxidative stress and inflammation and enhancing TGFβ/Smad3 mediators along with gastric protective mediators.
Key message
In vitro-grown true root cultures of
L. herbanicus
provide plant raw materials with potential application as a source of natural products against ethanol-induced gastric ulcer.</description><subject>albino</subject><subject>bioactive properties</subject><subject>Biological effects</subject><subject>biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Colorimetry</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Ethanol</subject><subject>Glutathione</subject><subject>Growth regulators</subject><subject>immunohistochemistry</subject><subject>Inflammation</subject><subject>Interleukin 18</subject><subject>Life Sciences</subject><subject>Lotus</subject><subject>malondialdehyde</subject><subject>Metabolites</subject><subject>Natural products</subject><subject>Omeprazole</subject><subject>Oral administration</subject><subject>Original Article</subject><subject>Oxidative stress</subject><subject>Plant extracts</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Prostaglandin E2</subject><subject>prostaglandins</subject><subject>Raw materials</subject><subject>Secondary metabolites</subject><subject>Smad3 protein</subject><subject>stomach ulcers</subject><subject>Superoxide dismutase</subject><subject>Ulcers</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kcFqGzEQhkVpIK6TF8hJ0Esvm2okrbTbWwlNYjDkkp7FrjyyZdYrV9Ka5DX6xNHGhUIPPQwziO__Z9BPyA2wW2BMf00AXLKKcVFKaV7pD2QBtRZVzaT8SBYMlK5UU-tL8imlPWNMCQkL8ns10pPPMdAcJ6QxhEztNOQpYqLB0XXIU6I77GM3elvGx2B3Kd9SfKHz2-YbPcbg_ODH7cwntGHcdPGVHjB3fRh8LkaFo8ehm5LvB6R-XnkKdNulsrjIM9rsT0jRuTJdkQvXDQmv__Ql-Xn_4_nusVo_Pazuvq8rK6TOldbQawlt07YoQGusG-u4QLtRDcgaJbqmB8mbRtRCuF5a6Bk4hZLbrt8osSRfzr7lgl8TpmwOPlkchm7EMCUjoBaKN1zJgn7-B92HKY7lOsNboUG2Us-G_EzZGFKK6Mwx-kP5CwPMzDGZc0ymxGTeYzK6iMRZlAo8bjH-tf6P6g1f55eO</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Abdelazeez, Walla M. A.</creator><creator>Maaty, Doaa A. M.</creator><creator>El-Swaify, Zeinab A.</creator><creator>Negm, Walaa A.</creator><creator>Selim, Hend M.</creator><creator>Aboueldis, Gamil R.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-4971-2300</orcidid><orcidid>https://orcid.org/0000-0002-0118-3967</orcidid><orcidid>https://orcid.org/0000-0003-0463-8047</orcidid><orcidid>https://orcid.org/0009-0000-5310-7571</orcidid><orcidid>https://orcid.org/0000-0003-0868-696X</orcidid></search><sort><creationdate>20240301</creationdate><title>In vitro true root cultures of Lotus hebranicus Hochst. ex brand: profiling of secondary metabolites and plausible in vivo gastroprotective effect</title><author>Abdelazeez, Walla M. A. ; Maaty, Doaa A. M. ; El-Swaify, Zeinab A. ; Negm, Walaa A. ; Selim, Hend M. ; Aboueldis, Gamil R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-771b7419899e3177e58cf23ecd68145e4ef8b142883533fb4c1b01f6e42cabd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>albino</topic><topic>bioactive properties</topic><topic>Biological effects</topic><topic>biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Colorimetry</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Ethanol</topic><topic>Glutathione</topic><topic>Growth regulators</topic><topic>immunohistochemistry</topic><topic>Inflammation</topic><topic>Interleukin 18</topic><topic>Life Sciences</topic><topic>Lotus</topic><topic>malondialdehyde</topic><topic>Metabolites</topic><topic>Natural products</topic><topic>Omeprazole</topic><topic>Oral administration</topic><topic>Original Article</topic><topic>Oxidative stress</topic><topic>Plant extracts</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Prostaglandin E2</topic><topic>prostaglandins</topic><topic>Raw materials</topic><topic>Secondary metabolites</topic><topic>Smad3 protein</topic><topic>stomach ulcers</topic><topic>Superoxide dismutase</topic><topic>Ulcers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdelazeez, Walla M. A.</creatorcontrib><creatorcontrib>Maaty, Doaa A. M.</creatorcontrib><creatorcontrib>El-Swaify, Zeinab A.</creatorcontrib><creatorcontrib>Negm, Walaa A.</creatorcontrib><creatorcontrib>Selim, Hend M.</creatorcontrib><creatorcontrib>Aboueldis, Gamil R.</creatorcontrib><collection>SpringerOpen (Open Access)</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdelazeez, Walla M. A.</au><au>Maaty, Doaa A. M.</au><au>El-Swaify, Zeinab A.</au><au>Negm, Walaa A.</au><au>Selim, Hend M.</au><au>Aboueldis, Gamil R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro true root cultures of Lotus hebranicus Hochst. ex brand: profiling of secondary metabolites and plausible in vivo gastroprotective effect</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2024-03-01</date><risdate>2024</risdate><volume>156</volume><issue>3</issue><spage>93</spage><epage>93</epage><pages>93-93</pages><artnum>93</artnum><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>In vitro, the
Lotus hebranicus
produces true root cultures under entirely growth regulator-free culture conditions. These roots are completely independent of the shoots and gives abundant amounts of biomass of true root culture as raw materials in a short time. Lately, gastric ulcer have gained an increasing interest as it affects millions around the world. So, this study aimed to screen and evaluate the chemical constituents of the
L. hebranicus
extract (plant and true root culture), in addition to studying the biological effect of these extracts on mice with ethanol-induced gastric ulcer and the possible role of mediating oxidative stress, inflammation and TGFβ/Smad3 as underlying mechanisms. Fifty-four Albino mice were assigned into nine groups (n = 6). Lotus was orally administered for seven successive days, followed by a single dose of oral ethanol for ulcer induction. Using histological assessment and immunohistochemical studies as well as colorimetric analysis, ELISA and qRT-PCR, the results revealed that ethanol-induced ulcers exhibited a substantial increase (
p
< 0.05) in malondialdehyde and interleukin 18 with a remarkable lessening in the gastro-protective mediators; superoxide dismutase, prostaglandin E2 and glutathione, parallel with an increase in TGFβ/Smad3 expressions relative to control. Pre-treatment with
L. hebranicus
showed a comparable result to those with omeprazole and showed a significant inhibitory effect on inflammatory mediators and significant elevation of the gastro-protective mediators relative to the positive control. In conclusion,
L. hebranicus
extract (plant/true root culture) has the potential to protect the gastric tissues against ethanol by inhibiting oxidative stress and inflammation and enhancing TGFβ/Smad3 mediators along with gastric protective mediators.
Key message
In vitro-grown true root cultures of
L. herbanicus
provide plant raw materials with potential application as a source of natural products against ethanol-induced gastric ulcer.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-023-02672-7</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4971-2300</orcidid><orcidid>https://orcid.org/0000-0002-0118-3967</orcidid><orcidid>https://orcid.org/0000-0003-0463-8047</orcidid><orcidid>https://orcid.org/0009-0000-5310-7571</orcidid><orcidid>https://orcid.org/0000-0003-0868-696X</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2024-03, Vol.156 (3), p.93-93, Article 93 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| source | Springer Online Journals |
| subjects | albino bioactive properties Biological effects biomass Biomedical and Life Sciences Colorimetry Enzyme-linked immunosorbent assay Ethanol Glutathione Growth regulators immunohistochemistry Inflammation Interleukin 18 Life Sciences Lotus malondialdehyde Metabolites Natural products Omeprazole Oral administration Original Article Oxidative stress Plant extracts Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Prostaglandin E2 prostaglandins Raw materials Secondary metabolites Smad3 protein stomach ulcers Superoxide dismutase Ulcers |
| title | In vitro true root cultures of Lotus hebranicus Hochst. ex brand: profiling of secondary metabolites and plausible in vivo gastroprotective effect |
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