Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil
The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples wer...
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| Veröffentlicht in: | Journal of soil science and plant nutrition 2020-09, Vol.20 (3), p.1178-1192 |
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| creator | Sena, Pâmella Thalita Souza do Nascimento, Tailane Ribeiro Lino, Jaynne de Oliveira Siqueira Oliveira, Gilmar Silva Ferreira Neto, Reginaldo Alves de Freitas, Ana Dolores Santiago Fernandes-Júnior, Paulo Ivan Martins, Lindete Míria Vieira |
| description | The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of
Bradyrhizobium
were subjected to the
nodC
symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical
Bradyrhizobium
cultural features. A total of 161, out of 251 isolates, showed positive amplification of the
nodC
gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera,
Bradyrhizobium
and
Microvirga
, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential. |
| doi_str_mv | 10.1007/s42729-020-00203-3 |
| format | Article |
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Bradyrhizobium
were subjected to the
nodC
symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical
Bradyrhizobium
cultural features. A total of 161, out of 251 isolates, showed positive amplification of the
nodC
gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera,
Bradyrhizobium
and
Microvirga
, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential.</description><identifier>ISSN: 0718-9508</identifier><identifier>EISSN: 0718-9516</identifier><identifier>DOI: 10.1007/s42729-020-00203-3</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agricultural land ; Agriculture ; Amplification ; Bacteria ; Biofilms ; Biomedical and Life Sciences ; Biotechnology ; Bradyrhizobium ; Classification ; Clusters ; Cowpeas ; Ecology ; Environment ; Experiments ; Farming systems ; Gene amplification ; Genetic diversity ; Legumes ; Life Sciences ; Microorganisms ; Nitrogen ; Nitrogen fixation ; Nitrogenation ; Nodulation ; Nodules ; Original Paper ; Plant Sciences ; Seeds ; Semi arid areas ; Semiarid zones ; Sodium chloride ; Soil fertility ; Soil Science & Conservation</subject><ispartof>Journal of soil science and plant nutrition, 2020-09, Vol.20 (3), p.1178-1192</ispartof><rights>Sociedad Chilena de la Ciencia del Suelo 2020</rights><rights>Sociedad Chilena de la Ciencia del Suelo 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-d131c871f6fbc2ef494fb5894d5b80876b50939e88a55decf400fed5fa805f393</citedby><cites>FETCH-LOGICAL-c319t-d131c871f6fbc2ef494fb5894d5b80876b50939e88a55decf400fed5fa805f393</cites><orcidid>0000-0001-5808-097X ; 0000-0002-6390-3720 ; 0000-0003-3261-4704 ; 0000-0002-7527-4087</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42729-020-00203-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2933610426?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,41464,42533,43781,51294</link.rule.ids></links><search><creatorcontrib>Sena, Pâmella Thalita Souza</creatorcontrib><creatorcontrib>do Nascimento, Tailane Ribeiro</creatorcontrib><creatorcontrib>Lino, Jaynne de Oliveira Siqueira</creatorcontrib><creatorcontrib>Oliveira, Gilmar Silva</creatorcontrib><creatorcontrib>Ferreira Neto, Reginaldo Alves</creatorcontrib><creatorcontrib>de Freitas, Ana Dolores Santiago</creatorcontrib><creatorcontrib>Fernandes-Júnior, Paulo Ivan</creatorcontrib><creatorcontrib>Martins, Lindete Míria Vieira</creatorcontrib><title>Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil</title><title>Journal of soil science and plant nutrition</title><addtitle>J Soil Sci Plant Nutr</addtitle><description>The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of
Bradyrhizobium
were subjected to the
nodC
symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical
Bradyrhizobium
cultural features. A total of 161, out of 251 isolates, showed positive amplification of the
nodC
gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera,
Bradyrhizobium
and
Microvirga
, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential.</description><subject>Agricultural land</subject><subject>Agriculture</subject><subject>Amplification</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Bradyrhizobium</subject><subject>Classification</subject><subject>Clusters</subject><subject>Cowpeas</subject><subject>Ecology</subject><subject>Environment</subject><subject>Experiments</subject><subject>Farming systems</subject><subject>Gene amplification</subject><subject>Genetic diversity</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Microorganisms</subject><subject>Nitrogen</subject><subject>Nitrogen fixation</subject><subject>Nitrogenation</subject><subject>Nodulation</subject><subject>Nodules</subject><subject>Original Paper</subject><subject>Plant Sciences</subject><subject>Seeds</subject><subject>Semi arid areas</subject><subject>Semiarid zones</subject><subject>Sodium chloride</subject><subject>Soil fertility</subject><subject>Soil Science & Conservation</subject><issn>0718-9508</issn><issn>0718-9516</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU9v1DAQxSMEElXpF-BkiWsD4zhO7GNZ_lUqAnXp2XKc8a6rJF7GXlW7n4UPi8tWcGMOM3N47_cOr6pec3jLAfp3qW36RtfQQA1liVo8q86g56rWknfP__6gXlYXKd1DGQUgoT-rfn2NE7r9ZOmSfd8eUohT3ARnp0tml5GtD_MQYg6OrbaWrMtI4WhziAuLnq3iww4tu92GYxyCZZ7izNYxTIndLSMS-xC8R8Ils6sNhRKT92SnQk0Z58TCwvIW2RrnYCmM7BY3T-T3ZI9helW98HZKePF0z6u7Tx9_rL7UN98-X6-ubmonuM71yAV3que-84Nr0Le69YNUuh3loED13SBBC41KWSlHdL4F8DhKbxVIL7Q4r96cuDuKP_eYsrmPe1pKpGm0EB2HtumKqjmpHMWUCL3ZUZgtHQwH81iEORVhSgfmTxFGFJM4mVIRLxukf-j_uH4DYtKNsw</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Sena, Pâmella Thalita 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Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil</title><author>Sena, Pâmella Thalita Souza ; do Nascimento, Tailane Ribeiro ; Lino, Jaynne de Oliveira Siqueira ; Oliveira, Gilmar Silva ; Ferreira Neto, Reginaldo Alves ; de Freitas, Ana Dolores Santiago ; Fernandes-Júnior, Paulo Ivan ; Martins, Lindete Míria Vieira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-d131c871f6fbc2ef494fb5894d5b80876b50939e88a55decf400fed5fa805f393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agricultural land</topic><topic>Agriculture</topic><topic>Amplification</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>Biomedical and Life 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Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of soil science and plant nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sena, Pâmella Thalita Souza</au><au>do Nascimento, Tailane Ribeiro</au><au>Lino, Jaynne de Oliveira Siqueira</au><au>Oliveira, Gilmar Silva</au><au>Ferreira Neto, Reginaldo Alves</au><au>de Freitas, Ana Dolores Santiago</au><au>Fernandes-Júnior, Paulo Ivan</au><au>Martins, Lindete Míria Vieira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil</atitle><jtitle>Journal of soil science and plant nutrition</jtitle><stitle>J Soil Sci Plant Nutr</stitle><date>2020-09-01</date><risdate>2020</risdate><volume>20</volume><issue>3</issue><spage>1178</spage><epage>1192</epage><pages>1178-1192</pages><issn>0718-9508</issn><eissn>0718-9516</eissn><abstract>The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of
Bradyrhizobium
were subjected to the
nodC
symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical
Bradyrhizobium
cultural features. A total of 161, out of 251 isolates, showed positive amplification of the
nodC
gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera,
Bradyrhizobium
and
Microvirga
, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s42729-020-00203-3</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-5808-097X</orcidid><orcidid>https://orcid.org/0000-0002-6390-3720</orcidid><orcidid>https://orcid.org/0000-0003-3261-4704</orcidid><orcidid>https://orcid.org/0000-0002-7527-4087</orcidid></addata></record> |
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| ispartof | Journal of soil science and plant nutrition, 2020-09, Vol.20 (3), p.1178-1192 |
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| language | eng |
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| subjects | Agricultural land Agriculture Amplification Bacteria Biofilms Biomedical and Life Sciences Biotechnology Bradyrhizobium Classification Clusters Cowpeas Ecology Environment Experiments Farming systems Gene amplification Genetic diversity Legumes Life Sciences Microorganisms Nitrogen Nitrogen fixation Nitrogenation Nodulation Nodules Original Paper Plant Sciences Seeds Semi arid areas Semiarid zones Sodium chloride Soil fertility Soil Science & Conservation |
| title | Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil |
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