Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth
The high incidence of cardiovascular disease has led to significant demand for synthetic vascular grafts in clinical applications. Anti-proliferation drugs are usually loaded into devices to achieve desirable anti-thrombosis effects after implantation. However, the non-selectiveness of these convent...
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| Veröffentlicht in: | Bio-design and manufacturing 2021-09, Vol.4 (3), p.469-478 |
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| creator | Chen, Shengyu Jia, Fan Zhao, Luying Qiu, Fuyu Jiang, Shaohua Ji, Jian Fu, Guosheng |
| description | The high incidence of cardiovascular disease has led to significant demand for synthetic vascular grafts in clinical applications. Anti-proliferation drugs are usually loaded into devices to achieve desirable anti-thrombosis effects after implantation. However, the non-selectiveness of these conventional drugs can lead to the failure of blood vessel reconstruction, leading to potential complications in the long term. To address this issue, an asymmetric membrane was constructed through electro-spinning techniques. The bilayer membrane loaded and effectively released nitric oxide (NO), as hoped, from only one side. Due to the short diffusion distance of NO, it exerted negligible effects on the other side of the membrane, thus allowing selective regulation of different cells on both sides. The released NO boosted the growth of endothelial cells (ECs) over smooth muscle cells (SMCs)—while on the side where NO was absent, SMCs grew into multilayers. The overall structure resembled a native blood vessel, with confluent ECs as the inner layer and layers of SMCs to support it. In addition, the membrane preserved the normal function of ECs, and at the same time did not exacerbate inflammatory responses. By preparing this material type that regulates cell behavior differentially, we describe a new method for its application in the cardiovascular field such as for artificial blood vessels.
Graphic abstract |
| doi_str_mv | 10.1007/s42242-021-00131-w |
| format | Article |
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Graphic abstract</description><identifier>ISSN: 2096-5524</identifier><identifier>EISSN: 2522-8552</identifier><identifier>DOI: 10.1007/s42242-021-00131-w</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Biocompatibility ; Biomaterials ; Biomedical Engineering and Bioengineering ; Blood vessels ; Cardiovascular diseases ; Cell growth ; Design of experiments ; Endothelial cells ; Engineering ; Immunosuppressive agents ; Inflammation ; Leukocytes ; Mechanical Engineering ; Membranes ; Nitric oxide ; Research Article ; Smooth muscle ; Sulfur content ; Thrombosis ; Transplants & implants</subject><ispartof>Bio-design and manufacturing, 2021-09, Vol.4 (3), p.469-478</ispartof><rights>Zhejiang University Press 2021</rights><rights>Zhejiang University Press 2021.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-c4eaa321894086f5157793863b1ff956f92a6e1f39f220e9a4042c9fe1a92dfe3</citedby><cites>FETCH-LOGICAL-c389t-c4eaa321894086f5157793863b1ff956f92a6e1f39f220e9a4042c9fe1a92dfe3</cites><orcidid>0000-0001-9870-4038</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/swsjyzz/swsjyzz.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42242-021-00131-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2932562790?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,41464,42533,43781,51294</link.rule.ids></links><search><creatorcontrib>Chen, Shengyu</creatorcontrib><creatorcontrib>Jia, Fan</creatorcontrib><creatorcontrib>Zhao, Luying</creatorcontrib><creatorcontrib>Qiu, Fuyu</creatorcontrib><creatorcontrib>Jiang, Shaohua</creatorcontrib><creatorcontrib>Ji, Jian</creatorcontrib><creatorcontrib>Fu, Guosheng</creatorcontrib><title>Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth</title><title>Bio-design and manufacturing</title><addtitle>Bio-des. Manuf</addtitle><description>The high incidence of cardiovascular disease has led to significant demand for synthetic vascular grafts in clinical applications. Anti-proliferation drugs are usually loaded into devices to achieve desirable anti-thrombosis effects after implantation. However, the non-selectiveness of these conventional drugs can lead to the failure of blood vessel reconstruction, leading to potential complications in the long term. To address this issue, an asymmetric membrane was constructed through electro-spinning techniques. The bilayer membrane loaded and effectively released nitric oxide (NO), as hoped, from only one side. Due to the short diffusion distance of NO, it exerted negligible effects on the other side of the membrane, thus allowing selective regulation of different cells on both sides. The released NO boosted the growth of endothelial cells (ECs) over smooth muscle cells (SMCs)—while on the side where NO was absent, SMCs grew into multilayers. The overall structure resembled a native blood vessel, with confluent ECs as the inner layer and layers of SMCs to support it. In addition, the membrane preserved the normal function of ECs, and at the same time did not exacerbate inflammatory responses. By preparing this material type that regulates cell behavior differentially, we describe a new method for its application in the cardiovascular field such as for artificial blood vessels.
Graphic abstract</description><subject>Biocompatibility</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Blood vessels</subject><subject>Cardiovascular diseases</subject><subject>Cell growth</subject><subject>Design of experiments</subject><subject>Endothelial cells</subject><subject>Engineering</subject><subject>Immunosuppressive agents</subject><subject>Inflammation</subject><subject>Leukocytes</subject><subject>Mechanical Engineering</subject><subject>Membranes</subject><subject>Nitric oxide</subject><subject>Research Article</subject><subject>Smooth muscle</subject><subject>Sulfur content</subject><subject>Thrombosis</subject><subject>Transplants & implants</subject><issn>2096-5524</issn><issn>2522-8552</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LAzEQhoMoWGr_gKeAFy-ryWS_cpRSP6DYi55DdjvZbtmPmmRZ2l9vdIXePM3APO878BByy9kDZyx7dDFADBEDHjHGBY_GCzKDBCDKkwQuw85kGoU1viYL5-qCCZnIkMxnZLtqsPS2d4eho6Yu0NIW28LqDulY-x3V7ti26G1d0vcNtdigdkhNb6nfId3WxqDFzte6CcdqaLSv-472hpbYNLSy_eh3N-TK6Mbh4m_Oyefz6mP5Gq03L2_Lp3VUilz6qIxRawE8lzHLU5PwJMukyFNRcGNkkhoJOkVuhDQADKWOWQylNMi1hK1BMSf3U--oO6O7Su37wXbho3Kj2x9PJwiOmGBMBPRuQg-2_xrQ-TMLUkCSQiZZoGCiymDIWTTqYOtW26PiTP24V5N7FXrVr3s1hpCYQi7AXYX2XP1P6hsXrYfj</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Chen, Shengyu</creator><creator>Jia, Fan</creator><creator>Zhao, Luying</creator><creator>Qiu, Fuyu</creator><creator>Jiang, Shaohua</creator><creator>Ji, Jian</creator><creator>Fu, Guosheng</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><general>Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China</general><general>Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Hangzhou 310016, China%MOE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China%Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><orcidid>https://orcid.org/0000-0001-9870-4038</orcidid></search><sort><creationdate>20210901</creationdate><title>Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth</title><author>Chen, Shengyu ; Jia, Fan ; Zhao, Luying ; Qiu, Fuyu ; Jiang, Shaohua ; Ji, Jian ; Fu, Guosheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-c4eaa321894086f5157793863b1ff956f92a6e1f39f220e9a4042c9fe1a92dfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biocompatibility</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Blood vessels</topic><topic>Cardiovascular diseases</topic><topic>Cell growth</topic><topic>Design of experiments</topic><topic>Endothelial cells</topic><topic>Engineering</topic><topic>Immunosuppressive agents</topic><topic>Inflammation</topic><topic>Leukocytes</topic><topic>Mechanical Engineering</topic><topic>Membranes</topic><topic>Nitric oxide</topic><topic>Research Article</topic><topic>Smooth muscle</topic><topic>Sulfur content</topic><topic>Thrombosis</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shengyu</creatorcontrib><creatorcontrib>Jia, Fan</creatorcontrib><creatorcontrib>Zhao, Luying</creatorcontrib><creatorcontrib>Qiu, Fuyu</creatorcontrib><creatorcontrib>Jiang, Shaohua</creatorcontrib><creatorcontrib>Ji, Jian</creatorcontrib><creatorcontrib>Fu, Guosheng</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Bio-design and manufacturing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shengyu</au><au>Jia, Fan</au><au>Zhao, Luying</au><au>Qiu, Fuyu</au><au>Jiang, Shaohua</au><au>Ji, Jian</au><au>Fu, Guosheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth</atitle><jtitle>Bio-design and manufacturing</jtitle><stitle>Bio-des. Manuf</stitle><date>2021-09-01</date><risdate>2021</risdate><volume>4</volume><issue>3</issue><spage>469</spage><epage>478</epage><pages>469-478</pages><issn>2096-5524</issn><eissn>2522-8552</eissn><abstract>The high incidence of cardiovascular disease has led to significant demand for synthetic vascular grafts in clinical applications. Anti-proliferation drugs are usually loaded into devices to achieve desirable anti-thrombosis effects after implantation. However, the non-selectiveness of these conventional drugs can lead to the failure of blood vessel reconstruction, leading to potential complications in the long term. To address this issue, an asymmetric membrane was constructed through electro-spinning techniques. The bilayer membrane loaded and effectively released nitric oxide (NO), as hoped, from only one side. Due to the short diffusion distance of NO, it exerted negligible effects on the other side of the membrane, thus allowing selective regulation of different cells on both sides. The released NO boosted the growth of endothelial cells (ECs) over smooth muscle cells (SMCs)—while on the side where NO was absent, SMCs grew into multilayers. The overall structure resembled a native blood vessel, with confluent ECs as the inner layer and layers of SMCs to support it. In addition, the membrane preserved the normal function of ECs, and at the same time did not exacerbate inflammatory responses. By preparing this material type that regulates cell behavior differentially, we describe a new method for its application in the cardiovascular field such as for artificial blood vessels.
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| ispartof | Bio-design and manufacturing, 2021-09, Vol.4 (3), p.469-478 |
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| source | Springer Nature - Complete Springer Journals; ProQuest Central |
| subjects | Biocompatibility Biomaterials Biomedical Engineering and Bioengineering Blood vessels Cardiovascular diseases Cell growth Design of experiments Endothelial cells Engineering Immunosuppressive agents Inflammation Leukocytes Mechanical Engineering Membranes Nitric oxide Research Article Smooth muscle Sulfur content Thrombosis Transplants & implants |
| title | Electrospun fiber membrane with asymmetric NO release for the differential regulation of cell growth |
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