Guided cell migration on a graded micropillar substrate
Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regenerat...
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| Veröffentlicht in: | Bio-design and manufacturing 2020-03, Vol.3 (1), p.60-70 |
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| creator | Krishnamoorthy, Srikumar Zhang, Zhengyi Xu, Changxue |
| description | Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of the micropillar spacing gradient, which enables the guided cell migration. Moreover, the effects of the micropillar spacing gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell spreading area. Finally, two issues significantly affecting the cell migration have been discussed: trapped cells between the micropillars and cell clusters. |
| doi_str_mv | 10.1007/s42242-020-00059-7 |
| format | Article |
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The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of the micropillar spacing gradient, which enables the guided cell migration. Moreover, the effects of the micropillar spacing gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell spreading area. Finally, two issues significantly affecting the cell migration have been discussed: trapped cells between the micropillars and cell clusters.</description><identifier>ISSN: 2096-5524</identifier><identifier>EISSN: 2522-8552</identifier><identifier>DOI: 10.1007/s42242-020-00059-7</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Biomaterials ; Biomedical Engineering and Bioengineering ; Cell adhesion & migration ; Cell migration ; Cell spreading ; Engineering ; Fibroblasts ; Infections ; Mechanical Engineering ; Microenvironments ; Photolithography ; Plasma ; Research Article ; Silicon wafers ; Silicones ; Topography ; Traumatic brain injury ; Wound healing</subject><ispartof>Bio-design and manufacturing, 2020-03, Vol.3 (1), p.60-70</ispartof><rights>Zhejiang University Press 2020</rights><rights>Zhejiang University Press 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-bbfc82d2afe1b6607d3fd987ad8e420caba08c7ba9a5ed4094d9c6029a78194c3</citedby><cites>FETCH-LOGICAL-c356t-bbfc82d2afe1b6607d3fd987ad8e420caba08c7ba9a5ed4094d9c6029a78194c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s42242-020-00059-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2932312106?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,21388,27924,27925,33744,41488,42557,43805,51319,64385,64389,72469</link.rule.ids></links><search><creatorcontrib>Krishnamoorthy, Srikumar</creatorcontrib><creatorcontrib>Zhang, Zhengyi</creatorcontrib><creatorcontrib>Xu, Changxue</creatorcontrib><title>Guided cell migration on a graded micropillar substrate</title><title>Bio-design and manufacturing</title><addtitle>Bio-des. Manuf</addtitle><description>Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of the micropillar spacing gradient, which enables the guided cell migration. Moreover, the effects of the micropillar spacing gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell spreading area. Finally, two issues significantly affecting the cell migration have been discussed: trapped cells between the micropillars and cell clusters.</description><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cell spreading</subject><subject>Engineering</subject><subject>Fibroblasts</subject><subject>Infections</subject><subject>Mechanical Engineering</subject><subject>Microenvironments</subject><subject>Photolithography</subject><subject>Plasma</subject><subject>Research Article</subject><subject>Silicon wafers</subject><subject>Silicones</subject><subject>Topography</subject><subject>Traumatic brain injury</subject><subject>Wound healing</subject><issn>2096-5524</issn><issn>2522-8552</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UMFKAzEQDaJgqf0BTwueo7OTZJMcpWgVCl70HLJJtkS23ZrsHvx7U1fwVhiYN8x7b4ZHyG0N9zWAfMgckSMFBAoAQlN5QRYoEKkSAi8LBt3QAvk1WeUcW2Ba6KJUCyI3U_TBVy70fbWPu2THOByqUrYqw2m1jy4Nx9j3NlV5avNYOOGGXHW2z2H115fk4_npff1Ct2-b1_XjljommpG2becUerRdqNumAelZ57WS1qvAEZxtLSgnW6utCJ6D5l67BlBbqWrNHVuSu9n3mIavKeTRfA5TOpSTBjVDVmMNTWHhzCqf5pxCZ44p7m36NjWYU0ZmzsiUjMxvRkYWEZtFuZAPu5D-rc-ofgAVRGk7</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Krishnamoorthy, Srikumar</creator><creator>Zhang, Zhengyi</creator><creator>Xu, Changxue</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope></search><sort><creationdate>20200301</creationdate><title>Guided cell migration on a graded micropillar substrate</title><author>Krishnamoorthy, Srikumar ; Zhang, Zhengyi ; Xu, Changxue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-bbfc82d2afe1b6607d3fd987ad8e420caba08c7ba9a5ed4094d9c6029a78194c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Cell adhesion & migration</topic><topic>Cell migration</topic><topic>Cell spreading</topic><topic>Engineering</topic><topic>Fibroblasts</topic><topic>Infections</topic><topic>Mechanical Engineering</topic><topic>Microenvironments</topic><topic>Photolithography</topic><topic>Plasma</topic><topic>Research Article</topic><topic>Silicon wafers</topic><topic>Silicones</topic><topic>Topography</topic><topic>Traumatic brain injury</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krishnamoorthy, Srikumar</creatorcontrib><creatorcontrib>Zhang, Zhengyi</creatorcontrib><creatorcontrib>Xu, Changxue</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><jtitle>Bio-design and manufacturing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krishnamoorthy, Srikumar</au><au>Zhang, Zhengyi</au><au>Xu, Changxue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guided cell migration on a graded micropillar substrate</atitle><jtitle>Bio-design and manufacturing</jtitle><stitle>Bio-des. Manuf</stitle><date>2020-03-01</date><risdate>2020</risdate><volume>3</volume><issue>1</issue><spage>60</spage><epage>70</epage><pages>60-70</pages><issn>2096-5524</issn><eissn>2522-8552</eissn><abstract>Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of the micropillar spacing gradient, which enables the guided cell migration. Moreover, the effects of the micropillar spacing gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell spreading area. Finally, two issues significantly affecting the cell migration have been discussed: trapped cells between the micropillars and cell clusters.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><doi>10.1007/s42242-020-00059-7</doi><tpages>11</tpages></addata></record> |
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| source | SpringerNature Journals; ProQuest Central UK/Ireland; ProQuest Central |
| subjects | Biomaterials Biomedical Engineering and Bioengineering Cell adhesion & migration Cell migration Cell spreading Engineering Fibroblasts Infections Mechanical Engineering Microenvironments Photolithography Plasma Research Article Silicon wafers Silicones Topography Traumatic brain injury Wound healing |
| title | Guided cell migration on a graded micropillar substrate |
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