Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae)
Feces of animals that forage on nectar and fruit, including many species of bats, often contain DNA that is low in quality and quantity. We developed an approach based on DNA from feces gathered passively to generate microsatellite data for individual lesser long-nosed bats ( Leptonycteris yerbabuen...
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| Veröffentlicht in: | Conservation genetics resources 2024-03, Vol.16 (1), p.159-171 |
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| creator | Walker, John-Lee Sky Steidl, Robert J. Wolf, Sandy A. Lee, Ming-Min Arnold, A. Elizabeth |
| description | Feces of animals that forage on nectar and fruit, including many species of bats, often contain DNA that is low in quality and quantity. We developed an approach based on DNA from feces gathered passively to generate microsatellite data for individual lesser long-nosed bats (
Leptonycteris yerbabuenae
), which are important pollinators for columnar cacti and agave across much of Mexico and in the southwestern U.S. We collected feces from roosts near the U.S-Mexico border and developed a two-step amplification approach to characterize five highly polymorphic microsatellite loci from fecal DNA. Addition of a multiplex PCR step improved amplification success and conserved DNA extracts with a minimal increase in cost. In our initial screening of 433 samples, five focal loci distinguished individuals reliably, with a probability of identity (i.e., the probability of two unrelated individuals having the same microsatellite profile by chance) of 7.5E-09. Repeated analyses revealed a genotyping error rate |
| doi_str_mv | 10.1007/s12686-023-01344-0 |
| format | Article |
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Leptonycteris yerbabuenae
), which are important pollinators for columnar cacti and agave across much of Mexico and in the southwestern U.S. We collected feces from roosts near the U.S-Mexico border and developed a two-step amplification approach to characterize five highly polymorphic microsatellite loci from fecal DNA. Addition of a multiplex PCR step improved amplification success and conserved DNA extracts with a minimal increase in cost. In our initial screening of 433 samples, five focal loci distinguished individuals reliably, with a probability of identity (i.e., the probability of two unrelated individuals having the same microsatellite profile by chance) of 7.5E-09. Repeated analyses revealed a genotyping error rate < 2%. We explore the benefits and limits of our approach for population studies of lesser long-nosed bats and other nectivorous and frugivorous species that provide key ecosystem services and are often of conservation concern.</description><identifier>ISSN: 1877-7260</identifier><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-023-01344-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Genetics and Genomics ; Bats ; Biodiversity ; Biomedical and Life Sciences ; Conservation Biology/Ecology ; Deoxyribonucleic acid ; DNA ; Ecology ; Evolutionary Biology ; Feces ; Genotyping ; Leptonycteris yerbabuenae ; Life Sciences ; Methods and Resources ; Nectar ; Plant Genetics and Genomics ; Pollinators ; Population studies</subject><ispartof>Conservation genetics resources, 2024-03, Vol.16 (1), p.159-171</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-6996ae371c7bebba160f3c57dd8ec1ca0561e99b4df8ba474bb1c3cb40cbfb983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-023-01344-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-023-01344-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Walker, John-Lee Sky</creatorcontrib><creatorcontrib>Steidl, Robert J.</creatorcontrib><creatorcontrib>Wolf, Sandy A.</creatorcontrib><creatorcontrib>Lee, Ming-Min</creatorcontrib><creatorcontrib>Arnold, A. Elizabeth</creatorcontrib><title>Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae)</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>Feces of animals that forage on nectar and fruit, including many species of bats, often contain DNA that is low in quality and quantity. We developed an approach based on DNA from feces gathered passively to generate microsatellite data for individual lesser long-nosed bats (
Leptonycteris yerbabuenae
), which are important pollinators for columnar cacti and agave across much of Mexico and in the southwestern U.S. We collected feces from roosts near the U.S-Mexico border and developed a two-step amplification approach to characterize five highly polymorphic microsatellite loci from fecal DNA. Addition of a multiplex PCR step improved amplification success and conserved DNA extracts with a minimal increase in cost. In our initial screening of 433 samples, five focal loci distinguished individuals reliably, with a probability of identity (i.e., the probability of two unrelated individuals having the same microsatellite profile by chance) of 7.5E-09. Repeated analyses revealed a genotyping error rate < 2%. We explore the benefits and limits of our approach for population studies of lesser long-nosed bats and other nectivorous and frugivorous species that provide key ecosystem services and are often of conservation concern.</description><subject>Animal Genetics and Genomics</subject><subject>Bats</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Conservation Biology/Ecology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Feces</subject><subject>Genotyping</subject><subject>Leptonycteris yerbabuenae</subject><subject>Life Sciences</subject><subject>Methods and Resources</subject><subject>Nectar</subject><subject>Plant Genetics and Genomics</subject><subject>Pollinators</subject><subject>Population studies</subject><issn>1877-7260</issn><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kLtOAzEQRVcIJELgB6gs0UBh8GOzXpdReEWKoIHasp3ZyNHGXmxvpHwCf82GIEFFNVPcc0Zzi-KSkltKiLhLlFV1hQnjmFBelpgcFSNaC4EFq8jxn_20OEtpTUhVc8ZGxed808WwhSXSm651jbM6u-BRaFADVrfo_mWKUt91IeaEfPDY-a1Obgto42wMSWdoW5cBrcCHvOucX-3hFlKCiNrgV9iHNPiNHgTXC-hy8DubIbqEdhCNNj14DTfnxUmj2wQXP3NcvD8-vM2e8eL1aT6bLrBlgmRcSVlp4IJaYcAYTSvScDsRy2UNllpNJhUFKU25bGqjS1EaQy23piTWNEbWfFxcHbzD3x89pKzWoY9-OKmYZJITybgcUuyQ2v-YIjSqi26j405RovaVq0PlaqhcfVeuyADxA5SGsF9B_FX_Q30BmQ6IXA</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Walker, John-Lee Sky</creator><creator>Steidl, Robert J.</creator><creator>Wolf, Sandy A.</creator><creator>Lee, Ming-Min</creator><creator>Arnold, A. Elizabeth</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20240301</creationdate><title>Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae)</title><author>Walker, John-Lee Sky ; Steidl, Robert J. ; Wolf, Sandy A. ; Lee, Ming-Min ; Arnold, A. 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Elizabeth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae)</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2024-03-01</date><risdate>2024</risdate><volume>16</volume><issue>1</issue><spage>159</spage><epage>171</epage><pages>159-171</pages><issn>1877-7260</issn><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>Feces of animals that forage on nectar and fruit, including many species of bats, often contain DNA that is low in quality and quantity. We developed an approach based on DNA from feces gathered passively to generate microsatellite data for individual lesser long-nosed bats (
Leptonycteris yerbabuenae
), which are important pollinators for columnar cacti and agave across much of Mexico and in the southwestern U.S. We collected feces from roosts near the U.S-Mexico border and developed a two-step amplification approach to characterize five highly polymorphic microsatellite loci from fecal DNA. Addition of a multiplex PCR step improved amplification success and conserved DNA extracts with a minimal increase in cost. In our initial screening of 433 samples, five focal loci distinguished individuals reliably, with a probability of identity (i.e., the probability of two unrelated individuals having the same microsatellite profile by chance) of 7.5E-09. Repeated analyses revealed a genotyping error rate < 2%. We explore the benefits and limits of our approach for population studies of lesser long-nosed bats and other nectivorous and frugivorous species that provide key ecosystem services and are often of conservation concern.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-023-01344-0</doi><tpages>13</tpages></addata></record> |
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| ispartof | Conservation genetics resources, 2024-03, Vol.16 (1), p.159-171 |
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| subjects | Animal Genetics and Genomics Bats Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Deoxyribonucleic acid DNA Ecology Evolutionary Biology Feces Genotyping Leptonycteris yerbabuenae Life Sciences Methods and Resources Nectar Plant Genetics and Genomics Pollinators Population studies |
| title | Improved amplification of fecal DNA supports non-invasive microsatellite genotyping of lesser long-nosed bats (Leptonycteris yerbabuenae) |
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