Development of subcutaneous system for thiocolchicoside and evaluation for in-vitro permeability studies

Selection of excipients or material to design a formulation is very important and critical step. These components decided the fate of dosage form, stability criteria and patient compliance. Selected components should be compatible with the drug as well and therapeutic effect of drug substance should be promoted. In this study the aim is to develop a parenteral dosage form for subcutaneous administration for permeability studies. All components were selected from the FDA-IIG link. It represents the potency of the component for the formulation. Selected components were Distilled water as an aqueous base, poly ethylene glycol (PEG-300) as a co-solvent, glycerol as a co-solvent, sorbitol as an osmogen, 0.9% w/v NaCl as an aqueous base and osmogen, 0.01 M PBS 7.4 as a buffer and butylated hydroxy anisole (BHA) as an anti-oxidant. All the components were taken on the behalf of their potency. Developed systems were evaluated for in-vitro permeability studies through pork subcutaneous tissue and egg or semi-permeable membrane. Maximum permeability of AF1, AF2 and AF3 through pork SC tissue was 82.64%, 66.03% and 76.93% after 120 minutes. Maximum permeability of AF1, AF2 and AF3 through semi-permeable membrane was 75.0%, 62.45% and 67.92% after 240 minutes. The permeability study data was examined with one-way ANOVA at 5% level of significance and permeability of drug found significantly different at every time interval.