A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues
To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. Our results revealed that these two prior steps do not c...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2024-02, Vol.6 (16), p.2212-2215 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Adamson, Christopher Liang, Yaquan Feng, Shiliu Ng, Allan Wee Ren Qiao, Yuan |
| description | To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. Our results revealed that these two prior steps do not confer ligand stereoselectivity; yet NAGK strictly discriminates against the disaccharide NOD2 agonists for phosphorylation
in vitro
, despite it being indispensable for the cellular NOD2-stimulating effects of these analogues, implying potential glycosidase cleavage as a novel intermediate step for cellular activation of NOD2.
To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. |
| doi_str_mv | 10.1039/d3cc05807g |
| format | Article |
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in vitro
, despite it being indispensable for the cellular NOD2-stimulating effects of these analogues, implying potential glycosidase cleavage as a novel intermediate step for cellular activation of NOD2.
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in vitro
, despite it being indispensable for the cellular NOD2-stimulating effects of these analogues, implying potential glycosidase cleavage as a novel intermediate step for cellular activation of NOD2.
To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation.</description><subject>Acetylmuramyl-Alanyl-Isoglutamine - metabolism</subject><subject>Acetylmuramyl-Alanyl-Isoglutamine - pharmacology</subject><subject>Disaccharides</subject><subject>Glycosidases</subject><subject>Ligands</subject><subject>Nod2 Signaling Adaptor Protein - metabolism</subject><subject>Phosphorylation</subject><subject>Stereoselectivity</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0VFr1TAUB_AgipvTF9-VwF5kUE2bpm0ex51uwnAvCr6V09OTu8y0qUmq3G9v7u6cYPKQQH6cJOfP2OtSvC-F1B9GiShUJ9rtE3ZcyqYuVN19f7rfK120slZH7EWMdyKPUnXP2ZHspFCtLI_ZfM7R-UiBO-9_cEjc2S3MI48LoTUWbdpx4wNHcm51EDhgsr8gWT9zb_iXm4uK_7bplsfdnG4pWeTTGmDaOT7ahZZkR-Iwg_PbleJL9syAi_TqYT1h3z59_Lq5Kq5vLj9vzq8LlLJKBSmDLSkphqED0rrSDcphqLUhhLYdRlE1FcKg8gRsOt0RoDGiHmuqNWh5wt4d6i7B_8z3pn6ycf8FmMmvsa90lXukVCMzPf2P3vk15Affq06VWtdNVmcHhcHHGMj0S7AThF1fin6fQn8hN5v7FC4zfvtQch0mGh_p37Zn8OYAQsTH038xyj-KtY3c</recordid><startdate>20240220</startdate><enddate>20240220</enddate><creator>Adamson, Christopher</creator><creator>Liang, Yaquan</creator><creator>Feng, Shiliu</creator><creator>Ng, Allan Wee Ren</creator><creator>Qiao, Yuan</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3044-2718</orcidid><orcidid>https://orcid.org/0009-0000-6062-6273</orcidid><orcidid>https://orcid.org/0000-0003-0188-0700</orcidid></search><sort><creationdate>20240220</creationdate><title>A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues</title><author>Adamson, Christopher ; Liang, Yaquan ; Feng, Shiliu ; Ng, Allan Wee Ren ; Qiao, Yuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-e5fc7e530bb8ae99296c3bb49feca77bd0262cab5b5bac6898eacff04d4e49a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetylmuramyl-Alanyl-Isoglutamine - metabolism</topic><topic>Acetylmuramyl-Alanyl-Isoglutamine - pharmacology</topic><topic>Disaccharides</topic><topic>Glycosidases</topic><topic>Ligands</topic><topic>Nod2 Signaling Adaptor Protein - metabolism</topic><topic>Phosphorylation</topic><topic>Stereoselectivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adamson, Christopher</creatorcontrib><creatorcontrib>Liang, Yaquan</creatorcontrib><creatorcontrib>Feng, Shiliu</creatorcontrib><creatorcontrib>Ng, Allan Wee Ren</creatorcontrib><creatorcontrib>Qiao, Yuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adamson, Christopher</au><au>Liang, Yaquan</au><au>Feng, Shiliu</au><au>Ng, Allan Wee Ren</au><au>Qiao, Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2024-02-20</date><risdate>2024</risdate><volume>6</volume><issue>16</issue><spage>2212</spage><epage>2215</epage><pages>2212-2215</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>To further understand the specificity of muramyl dipeptide (MDP) sensing by NOD2, we evaluated the compatibility of synthetic MDP analogues for cellular uptake and NAGK phosphorylation, the pre-requisite steps of intracellular NOD2 activation. Our results revealed that these two prior steps do not confer ligand stereoselectivity; yet NAGK strictly discriminates against the disaccharide NOD2 agonists for phosphorylation
in vitro
, despite it being indispensable for the cellular NOD2-stimulating effects of these analogues, implying potential glycosidase cleavage as a novel intermediate step for cellular activation of NOD2.
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| ispartof | Chemical communications (Cambridge, England), 2024-02, Vol.6 (16), p.2212-2215 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Acetylmuramyl-Alanyl-Isoglutamine - metabolism Acetylmuramyl-Alanyl-Isoglutamine - pharmacology Disaccharides Glycosidases Ligands Nod2 Signaling Adaptor Protein - metabolism Phosphorylation Stereoselectivity |
| title | A closer look at ligand specificity for cellular activation of NOD2 with synthetic muramyl dipeptide analogues |
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