Antifungal strains M1-8 and M6-4 as biocontrol agents against Aspergillus flavus on peanut kernels
Aspergillus flavus is the main pathogenic fungi for peanut kernels, and it has highly negative economic and health impacts. To explore the biological control agent against A. flavus, two antagonistic strains were screened from 70 bacteria isolates from moldy stuff and identified as Burkholderia plan...
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| Veröffentlicht in: | Quality assurance and safety of crops & food 2023-01, Vol.15 (4), p.116-124 |
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| creator | Yi, Yanjie Shan, Youtian Lou, Yu Ning, Zhiwen Zhang, Qingyao Yang, Yingao Liang, Yuqian Shi, Jinsheng Hou, Zhipeng |
| description | Aspergillus flavus is the main pathogenic fungi for peanut kernels, and it has highly negative economic and health impacts. To explore the biological control agent against A. flavus, two antagonistic strains were screened from 70 bacteria isolates from moldy stuff and identified as Burkholderia plantarii M1-8 and Burkholderia glumae M6-4. In vitro pathogen inhibition determination indicated culture filtrates of M1-8 and M6-4 had distinct inhibition zones and could decrease the mycelial growth of A. flavus. Furthermore, the biocontrol assay showed moldy rates of peanut seeds treated with culture filtrate were much lower than that of the control group. The biocontrol -efficacy of M1-8 and M6-4 culture filtrate reached 88.6% and 84.2%, respectively, which were higher than that of the sorbic acid treatment group. Moreover, culture filtrate of M1-8 was tolerant to proteinase K, high light, pH and temperature (below 70°C), and had good stability of antifungal activity. The results indicate that these two antifungal strains could be used as biocontrol agents for controlling A. flavus during the peanut supply and -storage period. |
| doi_str_mv | 10.15586/qas.v15i4.1186 |
| format | Article |
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To explore the biological control agent against A. flavus, two antagonistic strains were screened from 70 bacteria isolates from moldy stuff and identified as Burkholderia plantarii M1-8 and Burkholderia glumae M6-4. In vitro pathogen inhibition determination indicated culture filtrates of M1-8 and M6-4 had distinct inhibition zones and could decrease the mycelial growth of A. flavus. Furthermore, the biocontrol assay showed moldy rates of peanut seeds treated with culture filtrate were much lower than that of the control group. The biocontrol -efficacy of M1-8 and M6-4 culture filtrate reached 88.6% and 84.2%, respectively, which were higher than that of the sorbic acid treatment group. Moreover, culture filtrate of M1-8 was tolerant to proteinase K, high light, pH and temperature (below 70°C), and had good stability of antifungal activity. The results indicate that these two antifungal strains could be used as biocontrol agents for controlling A. flavus during the peanut supply and -storage period.</description><identifier>ISSN: 1757-8361</identifier><identifier>EISSN: 1757-837X</identifier><identifier>DOI: 10.15586/qas.v15i4.1186</identifier><language>eng</language><publisher>Brisbane: Codon Publications</publisher><subject>Antifungal activity ; Antifungal agents ; Aspergillus flavus ; Bacteria ; Biological control ; Endopeptidase K ; Filtrate ; Food contamination & poisoning ; Fungicides ; Legumes ; Maximum likelihood method ; Peanuts ; Proteinase ; Seeds ; Sorbic acid ; Strains (organisms) ; Temperature tolerance ; Variance analysis</subject><ispartof>Quality assurance and safety of crops & food, 2023-01, Vol.15 (4), p.116-124</ispartof><rights>2023. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). 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To explore the biological control agent against A. flavus, two antagonistic strains were screened from 70 bacteria isolates from moldy stuff and identified as Burkholderia plantarii M1-8 and Burkholderia glumae M6-4. In vitro pathogen inhibition determination indicated culture filtrates of M1-8 and M6-4 had distinct inhibition zones and could decrease the mycelial growth of A. flavus. Furthermore, the biocontrol assay showed moldy rates of peanut seeds treated with culture filtrate were much lower than that of the control group. The biocontrol -efficacy of M1-8 and M6-4 culture filtrate reached 88.6% and 84.2%, respectively, which were higher than that of the sorbic acid treatment group. Moreover, culture filtrate of M1-8 was tolerant to proteinase K, high light, pH and temperature (below 70°C), and had good stability of antifungal activity. 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Aspergillus flavus on peanut kernels</atitle><jtitle>Quality assurance and safety of crops & food</jtitle><date>2023-01-01</date><risdate>2023</risdate><volume>15</volume><issue>4</issue><spage>116</spage><epage>124</epage><pages>116-124</pages><issn>1757-8361</issn><eissn>1757-837X</eissn><abstract>Aspergillus flavus is the main pathogenic fungi for peanut kernels, and it has highly negative economic and health impacts. To explore the biological control agent against A. flavus, two antagonistic strains were screened from 70 bacteria isolates from moldy stuff and identified as Burkholderia plantarii M1-8 and Burkholderia glumae M6-4. In vitro pathogen inhibition determination indicated culture filtrates of M1-8 and M6-4 had distinct inhibition zones and could decrease the mycelial growth of A. flavus. Furthermore, the biocontrol assay showed moldy rates of peanut seeds treated with culture filtrate were much lower than that of the control group. The biocontrol -efficacy of M1-8 and M6-4 culture filtrate reached 88.6% and 84.2%, respectively, which were higher than that of the sorbic acid treatment group. Moreover, culture filtrate of M1-8 was tolerant to proteinase K, high light, pH and temperature (below 70°C), and had good stability of antifungal activity. The results indicate that these two antifungal strains could be used as biocontrol agents for controlling A. flavus during the peanut supply and -storage period.</abstract><cop>Brisbane</cop><pub>Codon Publications</pub><doi>10.15586/qas.v15i4.1186</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wageningen Academic Publishers Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Antifungal activity Antifungal agents Aspergillus flavus Bacteria Biological control Endopeptidase K Filtrate Food contamination & poisoning Fungicides Legumes Maximum likelihood method Peanuts Proteinase Seeds Sorbic acid Strains (organisms) Temperature tolerance Variance analysis |
| title | Antifungal strains M1-8 and M6-4 as biocontrol agents against Aspergillus flavus on peanut kernels |
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