Agrobacterium-medlated high-efficiency transformation of creeping bentgrass with herbicide resistance
We performedAgrobacterium-mediated genetic transformation of creeping bentgrass(Agrostis stolonifera L.) and produced herbicide-resistant transformants from commercial cultivars Crenshaw and Penncross. Seed-derived embryogenie calli were infected withA. tumefaciens EHA105 harboring pCAMBIA 3301, whi...
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| Veröffentlicht in: | Journal of plant biology = Singmul Hakhoe chi 2007-10, Vol.50 (5), p.577-585 |
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| container_title | Journal of plant biology = Singmul Hakhoe chi |
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| creator | Kim, Soo Jung Lee, Jee-Yeon Kim, Yong-Min Yang, Song-Sook Hwang, Ok-Jin Hong, Nam-Ju Kim, Kyung-Moon Lee, Hyo-Yeon Song, Pill-Soon Kim, Jeong-II |
| description | We performedAgrobacterium-mediated genetic transformation of creeping bentgrass(Agrostis stolonifera L.) and produced herbicide-resistant transformants from commercial cultivars Crenshaw and Penncross. Seed-derived embryogenie calli were infected withA. tumefaciens EHA105 harboring pCAMBIA 3301, which includes an intron-containinggus reporter and abar selection marker. To establish a stable system, we examined various factors that could potentially influence transformation efficiency during the pre-culture, infection, and co-cultivation steps. The addition of kinetin to the callus pre-culture media increased efficiency about three-fold. Once the optimum infection and co-cultivation conditions were identified, this protocol was used successfully to bulk-produce herbicide-resistant transgenic plants whose herbicide resistance was confirmed using the BASTA® resistance test. Southern blot analysis demonstrated integration and low copy numbers of the integrated transgenes, and northern blot analysis verified their expression. Thus, we have established an efficient genetic transformation system for creeping bentgrass and confirmed a high frequency of single-copy transgene integration and functional gene expression. |
| doi_str_mv | 10.1007/BF03030712 |
| format | Article |
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Seed-derived embryogenie calli were infected withA. tumefaciens EHA105 harboring pCAMBIA 3301, which includes an intron-containinggus reporter and abar selection marker. To establish a stable system, we examined various factors that could potentially influence transformation efficiency during the pre-culture, infection, and co-cultivation steps. The addition of kinetin to the callus pre-culture media increased efficiency about three-fold. Once the optimum infection and co-cultivation conditions were identified, this protocol was used successfully to bulk-produce herbicide-resistant transgenic plants whose herbicide resistance was confirmed using the BASTA® resistance test. Southern blot analysis demonstrated integration and low copy numbers of the integrated transgenes, and northern blot analysis verified their expression. Thus, we have established an efficient genetic transformation system for creeping bentgrass and confirmed a high frequency of single-copy transgene integration and functional gene expression.</description><identifier>ISSN: 1226-9239</identifier><identifier>EISSN: 1867-0725</identifier><identifier>DOI: 10.1007/BF03030712</identifier><language>eng</language><publisher>Heidelberg: Springer Nature B.V</publisher><subject>Callus ; Cultivars ; Cultivation ; Culture media ; Efficiency ; Gene expression ; Genetic transformation ; Herbicide resistance ; Herbicides ; Kinetin ; Transformations ; Transgenes ; Transgenic plants</subject><ispartof>Journal of plant biology = Singmul Hakhoe chi, 2007-10, Vol.50 (5), p.577-585</ispartof><rights>The Botanical Society of Korea 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1046-4a629f086cb35b40f95963e513ee00af27db099cb64d1644991b2e7cadda65ea3</citedby><cites>FETCH-LOGICAL-c1046-4a629f086cb35b40f95963e513ee00af27db099cb64d1644991b2e7cadda65ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2919505466?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,43781</link.rule.ids></links><search><creatorcontrib>Kim, Soo Jung</creatorcontrib><creatorcontrib>Lee, Jee-Yeon</creatorcontrib><creatorcontrib>Kim, Yong-Min</creatorcontrib><creatorcontrib>Yang, Song-Sook</creatorcontrib><creatorcontrib>Hwang, Ok-Jin</creatorcontrib><creatorcontrib>Hong, Nam-Ju</creatorcontrib><creatorcontrib>Kim, Kyung-Moon</creatorcontrib><creatorcontrib>Lee, Hyo-Yeon</creatorcontrib><creatorcontrib>Song, Pill-Soon</creatorcontrib><creatorcontrib>Kim, Jeong-II</creatorcontrib><title>Agrobacterium-medlated high-efficiency transformation of creeping bentgrass with herbicide resistance</title><title>Journal of plant biology = Singmul Hakhoe chi</title><description>We performedAgrobacterium-mediated genetic transformation of creeping bentgrass(Agrostis stolonifera L.) and produced herbicide-resistant transformants from commercial cultivars Crenshaw and Penncross. Seed-derived embryogenie calli were infected withA. tumefaciens EHA105 harboring pCAMBIA 3301, which includes an intron-containinggus reporter and abar selection marker. To establish a stable system, we examined various factors that could potentially influence transformation efficiency during the pre-culture, infection, and co-cultivation steps. The addition of kinetin to the callus pre-culture media increased efficiency about three-fold. Once the optimum infection and co-cultivation conditions were identified, this protocol was used successfully to bulk-produce herbicide-resistant transgenic plants whose herbicide resistance was confirmed using the BASTA® resistance test. Southern blot analysis demonstrated integration and low copy numbers of the integrated transgenes, and northern blot analysis verified their expression. Thus, we have established an efficient genetic transformation system for creeping bentgrass and confirmed a high frequency of single-copy transgene integration and functional gene expression.</description><subject>Callus</subject><subject>Cultivars</subject><subject>Cultivation</subject><subject>Culture media</subject><subject>Efficiency</subject><subject>Gene expression</subject><subject>Genetic transformation</subject><subject>Herbicide resistance</subject><subject>Herbicides</subject><subject>Kinetin</subject><subject>Transformations</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><issn>1226-9239</issn><issn>1867-0725</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpFUE9LwzAcDaLgnF78BAFvQvWXtEmb4xxOhYEXPZck_aXNWNuZpMi-vZUJ8g7vHd4feITcMnhgAOXj0wbyGSXjZ2TBKllmUHJxPmvOZaZ4ri7JVYw7AMl4VS0IrtowGm0TBj_1WY_NXidsaOfbLkPnvPU42CNNQQ_RjaHXyY8DHR21AfHgh5YaHFIbdIz026eOdhjMnGqQBow-Jj1YvCYXTu8j3vzxknxunj_Wr9n2_eVtvdpmlkEhs0JLrhxU0ppcmAKcEkrmKFiOCKAdLxsDSlkji4bJolCKGY6l1U2jpUCdL8ndqfcQxq8JY6p34xSGebLmiikBopBydt2fXDaMMQZ09SH4XodjzaD-vbH-vzH_AQuPZh0</recordid><startdate>200710</startdate><enddate>200710</enddate><creator>Kim, Soo Jung</creator><creator>Lee, Jee-Yeon</creator><creator>Kim, Yong-Min</creator><creator>Yang, Song-Sook</creator><creator>Hwang, Ok-Jin</creator><creator>Hong, Nam-Ju</creator><creator>Kim, Kyung-Moon</creator><creator>Lee, Hyo-Yeon</creator><creator>Song, Pill-Soon</creator><creator>Kim, Jeong-II</creator><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope></search><sort><creationdate>200710</creationdate><title>Agrobacterium-medlated high-efficiency transformation of creeping bentgrass with herbicide resistance</title><author>Kim, Soo Jung ; 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Seed-derived embryogenie calli were infected withA. tumefaciens EHA105 harboring pCAMBIA 3301, which includes an intron-containinggus reporter and abar selection marker. To establish a stable system, we examined various factors that could potentially influence transformation efficiency during the pre-culture, infection, and co-cultivation steps. The addition of kinetin to the callus pre-culture media increased efficiency about three-fold. Once the optimum infection and co-cultivation conditions were identified, this protocol was used successfully to bulk-produce herbicide-resistant transgenic plants whose herbicide resistance was confirmed using the BASTA® resistance test. Southern blot analysis demonstrated integration and low copy numbers of the integrated transgenes, and northern blot analysis verified their expression. 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| subjects | Callus Cultivars Cultivation Culture media Efficiency Gene expression Genetic transformation Herbicide resistance Herbicides Kinetin Transformations Transgenes Transgenic plants |
| title | Agrobacterium-medlated high-efficiency transformation of creeping bentgrass with herbicide resistance |
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