Effect of point-moxibustion on ghrelin and GHSR-1a expressions in lateral septal nucleus of rats with diabetic gastroparesis
Objective To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor 1a (GHSR-1a) in lateral septal nucleus of rats with diabetic gastroparesis (DGP), and to investigate the central regulatory mechanism of...
Gespeichert in:
Veröffentlicht in: | Journal of acupuncture and tuina science 2018-07, Vol.16 (4), p.216-222 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 222 |
---|---|
container_issue | 4 |
container_start_page | 216 |
container_title | Journal of acupuncture and tuina science |
container_volume | 16 |
creator | Liu, Mi Liu, Yi Liu, Wei-wei Wang, Chao Tan, Cheng-fu Liu, Li Peng, Yan Chang, Xiao-rong Li, Jing Yan, Jie |
description | Objective
To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor 1a (GHSR-1a) in lateral septal nucleus of rats with diabetic gastroparesis (DGP), and to investigate the central regulatory mechanism of DGP treatment with point-moxibustion.
Methods
Forty SPF-grade Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, an electroacupuncture (EA) group and a point-moxibustion group, with 10 rats in each group. A DGP rat model was established by intraperitoneal injection of 2% streptozotocin (STZ) with 8-week irregular high-sugar and high-fat diet in the model group, the EA group and the point-moxibustion group; and rats in the blank group were injected intraperitoneally with 0.1 mmoL/L (pH 4.5) citric acid-sodium citrate buffer with 8-week normal diet. Eight weeks later, rats in the EA group were treated by EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); while rats in the point-moxibustion group were treated by point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) for successive 15 d. Rats in the blank group and the model group were fixed as the control without intervention. After treatment, intestinal propulsion rate and gastric emptying rate were measured. The mRNA and protein expressions of ghrelin and GHSR-1a in the lateral septal nucleus were detected by real-time polymerase chain reaction (RT-PCR) and Western blot (WB).
Results
Compared with the blank group, the intestinal propulsion rate and gastric emptying rate of the model group were significantly lower (both
P |
doi_str_mv | 10.1007/s11726-018-1053-z |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2918730136</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2918730136</sourcerecordid><originalsourceid>FETCH-LOGICAL-c268t-735e86ffce3e807dea416e2f4d51d3096060dc6a900b4e0bd79d6b373e7510853</originalsourceid><addsrcrecordid>eNp1kFtLxDAQhYsouF5-gG8Bn6OTZps0j7KsF1gQvDyHtJ2ukW5bMyle8MebZQWfhIEzMOd8AyfLzgRcCAB9SULoXHEQJRdQSP61l82EMZKDNGY_7UrnXBZGH2ZHRK8AhVZ5Psu-l22LdWRDy8bB95Fvhg9fTRT90LM065eAne-Z6xt2c_v4wIVj-DEGJEoOYunUuYjBdYxwjEn6qe5woi0xuEjs3ccX1nhXYfQ1WzuKYRhdAng6yQ5a1xGe_upx9ny9fFrc8tX9zd3iasXrXJWRa1lgqdq2Rokl6AbdXCjM23lTiEaCUaCgqZUzANUcoWq0aVQltURdCCgLeZyd77hjGN4mpGhfhyn06aXNjSi1BCFVcomdqw4DUcDWjsFvXPi0Auy2ZLsr2aaS7bZk-5Uy-S5DyduvMfyR_w_9AM_mgWg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918730136</pqid></control><display><type>article</type><title>Effect of point-moxibustion on ghrelin and GHSR-1a expressions in lateral septal nucleus of rats with diabetic gastroparesis</title><source>Springer Nature - Complete Springer Journals</source><source>Alma/SFX Local Collection</source><source>ProQuest Central</source><creator>Liu, Mi ; Liu, Yi ; Liu, Wei-wei ; Wang, Chao ; Tan, Cheng-fu ; Liu, Li ; Peng, Yan ; Chang, Xiao-rong ; Li, Jing ; Yan, Jie</creator><creatorcontrib>Liu, Mi ; Liu, Yi ; Liu, Wei-wei ; Wang, Chao ; Tan, Cheng-fu ; Liu, Li ; Peng, Yan ; Chang, Xiao-rong ; Li, Jing ; Yan, Jie</creatorcontrib><description>Objective
To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor 1a (GHSR-1a) in lateral septal nucleus of rats with diabetic gastroparesis (DGP), and to investigate the central regulatory mechanism of DGP treatment with point-moxibustion.
Methods
Forty SPF-grade Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, an electroacupuncture (EA) group and a point-moxibustion group, with 10 rats in each group. A DGP rat model was established by intraperitoneal injection of 2% streptozotocin (STZ) with 8-week irregular high-sugar and high-fat diet in the model group, the EA group and the point-moxibustion group; and rats in the blank group were injected intraperitoneally with 0.1 mmoL/L (pH 4.5) citric acid-sodium citrate buffer with 8-week normal diet. Eight weeks later, rats in the EA group were treated by EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); while rats in the point-moxibustion group were treated by point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) for successive 15 d. Rats in the blank group and the model group were fixed as the control without intervention. After treatment, intestinal propulsion rate and gastric emptying rate were measured. The mRNA and protein expressions of ghrelin and GHSR-1a in the lateral septal nucleus were detected by real-time polymerase chain reaction (RT-PCR) and Western blot (WB).
Results
Compared with the blank group, the intestinal propulsion rate and gastric emptying rate of the model group were significantly lower (both
P
<0.01); compared with the model group, the intestinal propulsion rate and gastric emptying rate of the EA group and the point-moxibustion group increased significantly (all
P
<0.05). The mRNA and protein expressions of ghrelin and GHSR-1a were lower in the model group than those in the blank group (all
P
<0.01). The mRNA and protein expressions of ghrelin and GHSR-1a were significantly higher in the EA group and the point-moxibustion group than those in the model group (all
P
<0.05). There were no statistically significant differences between the EA group and the point-moxibustion group (all
P
>0.05).
Conclusion
Point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can increase the intestinal propulsion rate and gastric emptying rate of DGP rats, and promote the mRNA and protein expressions of ghrelin and GHSR-1a in the central nervous system. The mechanism may be related to the activation of ghrelin pathway in hypothalamic arcuate nucleus-lateral septal nucleus.</description><identifier>ISSN: 1672-3597</identifier><identifier>EISSN: 1993-0399</identifier><identifier>DOI: 10.1007/s11726-018-1053-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acupuncture ; Diabetes ; Medicine ; Medicine & Public Health ; Proteins ; Special Topic for 973 Program</subject><ispartof>Journal of acupuncture and tuina science, 2018-07, Vol.16 (4), p.216-222</ispartof><rights>Shanghai Research Institute of Acupuncture and Meridian 2018</rights><rights>Shanghai Research Institute of Acupuncture and Meridian 2018.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-735e86ffce3e807dea416e2f4d51d3096060dc6a900b4e0bd79d6b373e7510853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11726-018-1053-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918730136?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21369,27903,27904,33723,41467,42536,43784,51297</link.rule.ids></links><search><creatorcontrib>Liu, Mi</creatorcontrib><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Liu, Wei-wei</creatorcontrib><creatorcontrib>Wang, Chao</creatorcontrib><creatorcontrib>Tan, Cheng-fu</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Peng, Yan</creatorcontrib><creatorcontrib>Chang, Xiao-rong</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Yan, Jie</creatorcontrib><title>Effect of point-moxibustion on ghrelin and GHSR-1a expressions in lateral septal nucleus of rats with diabetic gastroparesis</title><title>Journal of acupuncture and tuina science</title><addtitle>J. Acupunct. Tuina. Sci</addtitle><description>Objective
To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor 1a (GHSR-1a) in lateral septal nucleus of rats with diabetic gastroparesis (DGP), and to investigate the central regulatory mechanism of DGP treatment with point-moxibustion.
Methods
Forty SPF-grade Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, an electroacupuncture (EA) group and a point-moxibustion group, with 10 rats in each group. A DGP rat model was established by intraperitoneal injection of 2% streptozotocin (STZ) with 8-week irregular high-sugar and high-fat diet in the model group, the EA group and the point-moxibustion group; and rats in the blank group were injected intraperitoneally with 0.1 mmoL/L (pH 4.5) citric acid-sodium citrate buffer with 8-week normal diet. Eight weeks later, rats in the EA group were treated by EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); while rats in the point-moxibustion group were treated by point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) for successive 15 d. Rats in the blank group and the model group were fixed as the control without intervention. After treatment, intestinal propulsion rate and gastric emptying rate were measured. The mRNA and protein expressions of ghrelin and GHSR-1a in the lateral septal nucleus were detected by real-time polymerase chain reaction (RT-PCR) and Western blot (WB).
Results
Compared with the blank group, the intestinal propulsion rate and gastric emptying rate of the model group were significantly lower (both
P
<0.01); compared with the model group, the intestinal propulsion rate and gastric emptying rate of the EA group and the point-moxibustion group increased significantly (all
P
<0.05). The mRNA and protein expressions of ghrelin and GHSR-1a were lower in the model group than those in the blank group (all
P
<0.01). The mRNA and protein expressions of ghrelin and GHSR-1a were significantly higher in the EA group and the point-moxibustion group than those in the model group (all
P
<0.05). There were no statistically significant differences between the EA group and the point-moxibustion group (all
P
>0.05).
Conclusion
Point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can increase the intestinal propulsion rate and gastric emptying rate of DGP rats, and promote the mRNA and protein expressions of ghrelin and GHSR-1a in the central nervous system. The mechanism may be related to the activation of ghrelin pathway in hypothalamic arcuate nucleus-lateral septal nucleus.</description><subject>Acupuncture</subject><subject>Diabetes</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Proteins</subject><subject>Special Topic for 973 Program</subject><issn>1672-3597</issn><issn>1993-0399</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kFtLxDAQhYsouF5-gG8Bn6OTZps0j7KsF1gQvDyHtJ2ukW5bMyle8MebZQWfhIEzMOd8AyfLzgRcCAB9SULoXHEQJRdQSP61l82EMZKDNGY_7UrnXBZGH2ZHRK8AhVZ5Psu-l22LdWRDy8bB95Fvhg9fTRT90LM065eAne-Z6xt2c_v4wIVj-DEGJEoOYunUuYjBdYxwjEn6qe5woi0xuEjs3ccX1nhXYfQ1WzuKYRhdAng6yQ5a1xGe_upx9ny9fFrc8tX9zd3iasXrXJWRa1lgqdq2Rokl6AbdXCjM23lTiEaCUaCgqZUzANUcoWq0aVQltURdCCgLeZyd77hjGN4mpGhfhyn06aXNjSi1BCFVcomdqw4DUcDWjsFvXPi0Auy2ZLsr2aaS7bZk-5Uy-S5DyduvMfyR_w_9AM_mgWg</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Liu, Mi</creator><creator>Liu, Yi</creator><creator>Liu, Wei-wei</creator><creator>Wang, Chao</creator><creator>Tan, Cheng-fu</creator><creator>Liu, Li</creator><creator>Peng, Yan</creator><creator>Chang, Xiao-rong</creator><creator>Li, Jing</creator><creator>Yan, Jie</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20180701</creationdate><title>Effect of point-moxibustion on ghrelin and GHSR-1a expressions in lateral septal nucleus of rats with diabetic gastroparesis</title><author>Liu, Mi ; Liu, Yi ; Liu, Wei-wei ; Wang, Chao ; Tan, Cheng-fu ; Liu, Li ; Peng, Yan ; Chang, Xiao-rong ; Li, Jing ; Yan, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-735e86ffce3e807dea416e2f4d51d3096060dc6a900b4e0bd79d6b373e7510853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acupuncture</topic><topic>Diabetes</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Proteins</topic><topic>Special Topic for 973 Program</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Mi</creatorcontrib><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Liu, Wei-wei</creatorcontrib><creatorcontrib>Wang, Chao</creatorcontrib><creatorcontrib>Tan, Cheng-fu</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Peng, Yan</creatorcontrib><creatorcontrib>Chang, Xiao-rong</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Yan, Jie</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Journal of acupuncture and tuina science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Mi</au><au>Liu, Yi</au><au>Liu, Wei-wei</au><au>Wang, Chao</au><au>Tan, Cheng-fu</au><au>Liu, Li</au><au>Peng, Yan</au><au>Chang, Xiao-rong</au><au>Li, Jing</au><au>Yan, Jie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of point-moxibustion on ghrelin and GHSR-1a expressions in lateral septal nucleus of rats with diabetic gastroparesis</atitle><jtitle>Journal of acupuncture and tuina science</jtitle><stitle>J. Acupunct. Tuina. Sci</stitle><date>2018-07-01</date><risdate>2018</risdate><volume>16</volume><issue>4</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>1672-3597</issn><eissn>1993-0399</eissn><abstract>Objective
To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor 1a (GHSR-1a) in lateral septal nucleus of rats with diabetic gastroparesis (DGP), and to investigate the central regulatory mechanism of DGP treatment with point-moxibustion.
Methods
Forty SPF-grade Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, an electroacupuncture (EA) group and a point-moxibustion group, with 10 rats in each group. A DGP rat model was established by intraperitoneal injection of 2% streptozotocin (STZ) with 8-week irregular high-sugar and high-fat diet in the model group, the EA group and the point-moxibustion group; and rats in the blank group were injected intraperitoneally with 0.1 mmoL/L (pH 4.5) citric acid-sodium citrate buffer with 8-week normal diet. Eight weeks later, rats in the EA group were treated by EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); while rats in the point-moxibustion group were treated by point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) for successive 15 d. Rats in the blank group and the model group were fixed as the control without intervention. After treatment, intestinal propulsion rate and gastric emptying rate were measured. The mRNA and protein expressions of ghrelin and GHSR-1a in the lateral septal nucleus were detected by real-time polymerase chain reaction (RT-PCR) and Western blot (WB).
Results
Compared with the blank group, the intestinal propulsion rate and gastric emptying rate of the model group were significantly lower (both
P
<0.01); compared with the model group, the intestinal propulsion rate and gastric emptying rate of the EA group and the point-moxibustion group increased significantly (all
P
<0.05). The mRNA and protein expressions of ghrelin and GHSR-1a were lower in the model group than those in the blank group (all
P
<0.01). The mRNA and protein expressions of ghrelin and GHSR-1a were significantly higher in the EA group and the point-moxibustion group than those in the model group (all
P
<0.05). There were no statistically significant differences between the EA group and the point-moxibustion group (all
P
>0.05).
Conclusion
Point-moxibustion at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can increase the intestinal propulsion rate and gastric emptying rate of DGP rats, and promote the mRNA and protein expressions of ghrelin and GHSR-1a in the central nervous system. The mechanism may be related to the activation of ghrelin pathway in hypothalamic arcuate nucleus-lateral septal nucleus.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11726-018-1053-z</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1672-3597 |
ispartof | Journal of acupuncture and tuina science, 2018-07, Vol.16 (4), p.216-222 |
issn | 1672-3597 1993-0399 |
language | eng |
recordid | cdi_proquest_journals_2918730136 |
source | Springer Nature - Complete Springer Journals; Alma/SFX Local Collection; ProQuest Central |
subjects | Acupuncture Diabetes Medicine Medicine & Public Health Proteins Special Topic for 973 Program |
title | Effect of point-moxibustion on ghrelin and GHSR-1a expressions in lateral septal nucleus of rats with diabetic gastroparesis |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T16%3A06%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20point-moxibustion%20on%20ghrelin%20and%20GHSR-1a%20expressions%20in%20lateral%20septal%20nucleus%20of%20rats%20with%20diabetic%20gastroparesis&rft.jtitle=Journal%20of%20acupuncture%20and%20tuina%20science&rft.au=Liu,%20Mi&rft.date=2018-07-01&rft.volume=16&rft.issue=4&rft.spage=216&rft.epage=222&rft.pages=216-222&rft.issn=1672-3597&rft.eissn=1993-0399&rft_id=info:doi/10.1007/s11726-018-1053-z&rft_dat=%3Cproquest_cross%3E2918730136%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2918730136&rft_id=info:pmid/&rfr_iscdi=true |