Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1, mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome
Objective To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore...
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| creator | Bi, Ding-yan Ning, Jiong-jie Xu, Yin Luo, Yan Li, Mu-qing Wang, Yun-hui Yi, Zhan |
| description | Objective
To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome.
Methods
Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C 200% concentrated
Da Huang
(
Radix et Rhizoma Rhei
) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry.
Results
After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all
P |
| doi_str_mv | 10.1007/s11726-018-1015-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2918730059</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2918730059</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-28d6cc476b9d249c237bc1240ff69900a00d32e90ee5989ea051e0e8e382ece3</originalsourceid><addsrcrecordid>eNp1UctOZCEQvZloMr4-wB2JWxkL6PtgaTrqmJi46T2hoWgxfeEO0HH6j_xMudNjXJmQ1KE4p6ic0zSXDH4xgP4mM9bzjgIbKAPW0vZHc8KkFBSElEcVdz2nopX9z-Y051eAtu84P2ne75xDUzKJjlhfccJQiI0Z_7U2Pmww0Umn4ouPAS0Z41-_3uX5RuopCV30W-K0KTERdk3GnfGBtLdLooMlOHmLadRbsknxrbx8EhManGZQuUnXBd58fczTFjEQi84bj8HsSd4Hm-KI582x09uMF__rWbO6v1stf9On54fH5e0TNYJ1hfLBdsYs-m4tLV9Iw0W_NowvwLlOSgANYAVHCYitHCRqaBkCDigGXjcSZ83VYeyU4p8d5qJe4y6F-qPikg29qM7JymIHlkkx5-qAmpIfddorBmrOQx3yUDUPNeeh2qrhB02u3NnVr8nfiz4AGUyQwA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918730059</pqid></control><display><type>article</type><title>Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1, mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome</title><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><source>ProQuest Central</source><creator>Bi, Ding-yan ; Ning, Jiong-jie ; Xu, Yin ; Luo, Yan ; Li, Mu-qing ; Wang, Yun-hui ; Yi, Zhan</creator><creatorcontrib>Bi, Ding-yan ; Ning, Jiong-jie ; Xu, Yin ; Luo, Yan ; Li, Mu-qing ; Wang, Yun-hui ; Yi, Zhan</creatorcontrib><description>Objective
To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome.
Methods
Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C 200% concentrated
Da Huang
(
Radix et Rhizoma Rhei
) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry.
Results
After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all
P
<0.01); compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased (all
P
<0.01). Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased (all
P
<0.01), however, there was no significant difference between group C2 and C3 (all
P
>0.05). The mechanism may be related to the increase of serum TFF1 and MUC5AC levels and activation of EGFR protein.
Conclusion
Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone ginger-partitioned moxibustion groups.</description><identifier>ISSN: 1672-3597</identifier><identifier>EISSN: 1993-0399</identifier><identifier>DOI: 10.1007/s11726-018-1015-5</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acupuncture ; Basic Study ; Epidermal growth factor ; Medicine ; Medicine & Public Health ; Protein expression ; Proteins ; Spleen</subject><ispartof>Journal of acupuncture and tuina science, 2018-02, Vol.16 (1), p.1-7</ispartof><rights>Shanghai Research Institute of Acupuncture and Meridian 2018</rights><rights>Shanghai Research Institute of Acupuncture and Meridian 2018.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-28d6cc476b9d249c237bc1240ff69900a00d32e90ee5989ea051e0e8e382ece3</citedby><cites>FETCH-LOGICAL-c316t-28d6cc476b9d249c237bc1240ff69900a00d32e90ee5989ea051e0e8e382ece3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11726-018-1015-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918730059?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,41464,42533,43781,51294</link.rule.ids></links><search><creatorcontrib>Bi, Ding-yan</creatorcontrib><creatorcontrib>Ning, Jiong-jie</creatorcontrib><creatorcontrib>Xu, Yin</creatorcontrib><creatorcontrib>Luo, Yan</creatorcontrib><creatorcontrib>Li, Mu-qing</creatorcontrib><creatorcontrib>Wang, Yun-hui</creatorcontrib><creatorcontrib>Yi, Zhan</creatorcontrib><title>Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1, mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome</title><title>Journal of acupuncture and tuina science</title><addtitle>J. Acupunct. Tuina. Sci</addtitle><description>Objective
To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome.
Methods
Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C 200% concentrated
Da Huang
(
Radix et Rhizoma Rhei
) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry.
Results
After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all
P
<0.01); compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased (all
P
<0.01). Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased (all
P
<0.01), however, there was no significant difference between group C2 and C3 (all
P
>0.05). The mechanism may be related to the increase of serum TFF1 and MUC5AC levels and activation of EGFR protein.
Conclusion
Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone ginger-partitioned moxibustion groups.</description><subject>Acupuncture</subject><subject>Basic Study</subject><subject>Epidermal growth factor</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Spleen</subject><issn>1672-3597</issn><issn>1993-0399</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1UctOZCEQvZloMr4-wB2JWxkL6PtgaTrqmJi46T2hoWgxfeEO0HH6j_xMudNjXJmQ1KE4p6ic0zSXDH4xgP4mM9bzjgIbKAPW0vZHc8KkFBSElEcVdz2nopX9z-Y051eAtu84P2ne75xDUzKJjlhfccJQiI0Z_7U2Pmww0Umn4ouPAS0Z41-_3uX5RuopCV30W-K0KTERdk3GnfGBtLdLooMlOHmLadRbsknxrbx8EhManGZQuUnXBd58fczTFjEQi84bj8HsSd4Hm-KI582x09uMF__rWbO6v1stf9On54fH5e0TNYJ1hfLBdsYs-m4tLV9Iw0W_NowvwLlOSgANYAVHCYitHCRqaBkCDigGXjcSZ83VYeyU4p8d5qJe4y6F-qPikg29qM7JymIHlkkx5-qAmpIfddorBmrOQx3yUDUPNeeh2qrhB02u3NnVr8nfiz4AGUyQwA</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Bi, Ding-yan</creator><creator>Ning, Jiong-jie</creator><creator>Xu, Yin</creator><creator>Luo, Yan</creator><creator>Li, Mu-qing</creator><creator>Wang, Yun-hui</creator><creator>Yi, Zhan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20180201</creationdate><title>Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1, mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome</title><author>Bi, Ding-yan ; Ning, Jiong-jie ; Xu, Yin ; Luo, Yan ; Li, Mu-qing ; Wang, Yun-hui ; Yi, Zhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-28d6cc476b9d249c237bc1240ff69900a00d32e90ee5989ea051e0e8e382ece3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acupuncture</topic><topic>Basic Study</topic><topic>Epidermal growth factor</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Spleen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bi, Ding-yan</creatorcontrib><creatorcontrib>Ning, Jiong-jie</creatorcontrib><creatorcontrib>Xu, Yin</creatorcontrib><creatorcontrib>Luo, Yan</creatorcontrib><creatorcontrib>Li, Mu-qing</creatorcontrib><creatorcontrib>Wang, Yun-hui</creatorcontrib><creatorcontrib>Yi, Zhan</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Journal of acupuncture and tuina science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bi, Ding-yan</au><au>Ning, Jiong-jie</au><au>Xu, Yin</au><au>Luo, Yan</au><au>Li, Mu-qing</au><au>Wang, Yun-hui</au><au>Yi, Zhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1, mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome</atitle><jtitle>Journal of acupuncture and tuina science</jtitle><stitle>J. Acupunct. Tuina. Sci</stitle><date>2018-02-01</date><risdate>2018</risdate><volume>16</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1672-3597</issn><eissn>1993-0399</eissn><abstract>Objective
To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome.
Methods
Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C 200% concentrated
Da Huang
(
Radix et Rhizoma Rhei
) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry.
Results
After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all
P
<0.01); compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased (all
P
<0.01). Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased (all
P
<0.01), however, there was no significant difference between group C2 and C3 (all
P
>0.05). The mechanism may be related to the increase of serum TFF1 and MUC5AC levels and activation of EGFR protein.
Conclusion
Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone ginger-partitioned moxibustion groups.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s11726-018-1015-5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-3597 |
| ispartof | Journal of acupuncture and tuina science, 2018-02, Vol.16 (1), p.1-7 |
| issn | 1672-3597 1993-0399 |
| language | eng |
| recordid | cdi_proquest_journals_2918730059 |
| source | SpringerLink Journals; Alma/SFX Local Collection; ProQuest Central |
| subjects | Acupuncture Basic Study Epidermal growth factor Medicine Medicine & Public Health Protein expression Proteins Spleen |
| title | Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1, mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome |
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