Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides
In this work, we demonstrate for the first time, a method to synthesize phenylboronic acid-Fe304@polydopamine （Fe3O4@ PDA-PBA） magnetic microspheres via the combination of mussel-inspired polydopamine coating and click chemistry. Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microsph...
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| Veröffentlicht in: | Science China. Chemistry 2015-06, Vol.58 (6), p.1056-1064 |
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| creator | Zheng, Jiangnan Lin, Zian Zhang, Lan Yang, Huanghao |
| description | In this work, we demonstrate for the first time, a method to synthesize phenylboronic acid-Fe304@polydopamine （Fe3O4@ PDA-PBA） magnetic microspheres via the combination of mussel-inspired polydopamine coating and click chemistry. Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microspheres with a core diameter of -240 nm and a shell thickness of -13 nm were obtained as identified by the characterization of the morphology, structure and composition of the synthesized microspheres. We evaluated the selectivity and binding capacity of the Fe3O4@PDA-PBA magnetic microsphcres by using standard glycoproteins （ovalbumin, immunoglobulin G and catalase） and nonglycoproteins （human serum albumin, bovine hemoglobin, myoglobin, lysozyme, and ribonuclease A） as model proteins. Adsorption experiments, SDS-PAGE and mass spectrometry analysis demonstrated that the Fe3O4@PDA-PBA magnetic microspheres had much high binding capacity and selectivity for glycoproteins/glycopeptides compared to nonglycoproteins/nonglycopeptides. In addition, the practicability of the Fe3O4@PDA-PBA magnetic microspheres was further assessed by selective capture of glycoproteins from healthy hu- man serum. The good results demonstrated its potential in glycoproteome analysis. |
| doi_str_mv | 10.1007/s11426-014-5286-5 |
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Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microspheres with a core diameter of -240 nm and a shell thickness of -13 nm were obtained as identified by the characterization of the morphology, structure and composition of the synthesized microspheres. We evaluated the selectivity and binding capacity of the Fe3O4@PDA-PBA magnetic microsphcres by using standard glycoproteins （ovalbumin, immunoglobulin G and catalase） and nonglycoproteins （human serum albumin, bovine hemoglobin, myoglobin, lysozyme, and ribonuclease A） as model proteins. Adsorption experiments, SDS-PAGE and mass spectrometry analysis demonstrated that the Fe3O4@PDA-PBA magnetic microspheres had much high binding capacity and selectivity for glycoproteins/glycopeptides compared to nonglycoproteins/nonglycopeptides. In addition, the practicability of the Fe3O4@PDA-PBA magnetic microspheres was further assessed by selective capture of glycoproteins from healthy hu- man serum. The good results demonstrated its potential in glycoproteome analysis.</description><identifier>ISSN: 1674-7291</identifier><identifier>EISSN: 1869-1870</identifier><identifier>DOI: 10.1007/s11426-014-5286-5</identifier><language>eng</language><publisher>Beijing: Science China Press</publisher><subject>Binding ; Catalase ; Chemical synthesis ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Core-shell structure ; Diameters ; Glycopeptides ; Glycoproteins ; IgG antibody ; Iron oxides ; Lysozyme ; Mass spectrometry ; Microspheres ; Myoglobins ; Ovalbumin ; Ribonuclease A ; SDS-PAGE ; Selectivity ; Serum albumin ; 人血清白蛋白 ; 固定化 ; 多巴胺 ; 磁性微球 ; 糖肽 ; 糖蛋白 ; 苯基硼酸</subject><ispartof>Science China. Chemistry, 2015-06, Vol.58 (6), p.1056-1064</ispartof><rights>Science China Press and Springer-Verlag Berlin Heidelberg 2015</rights><rights>Science China Press and Springer-Verlag Berlin Heidelberg 2015.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-8a93865a001bbda60d0cf36750cb91fe50956757b62ed996d77d793818854f633</citedby><cites>FETCH-LOGICAL-c413t-8a93865a001bbda60d0cf36750cb91fe50956757b62ed996d77d793818854f633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60113X/60113X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11426-014-5286-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918575763?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21368,21369,21370,21371,23236,27903,27904,33509,33682,33723,33984,34293,41467,42536,43638,43766,43784,43932,44046,51297,64361,64365,72215</link.rule.ids></links><search><creatorcontrib>Zheng, Jiangnan</creatorcontrib><creatorcontrib>Lin, Zian</creatorcontrib><creatorcontrib>Zhang, Lan</creatorcontrib><creatorcontrib>Yang, Huanghao</creatorcontrib><title>Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides</title><title>Science China. Chemistry</title><addtitle>Sci. China Chem</addtitle><addtitle>SCIENCE CHINA Chemistry</addtitle><description>In this work, we demonstrate for the first time, a method to synthesize phenylboronic acid-Fe304@polydopamine （Fe3O4@ PDA-PBA） magnetic microspheres via the combination of mussel-inspired polydopamine coating and click chemistry. Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microspheres with a core diameter of -240 nm and a shell thickness of -13 nm were obtained as identified by the characterization of the morphology, structure and composition of the synthesized microspheres. We evaluated the selectivity and binding capacity of the Fe3O4@PDA-PBA magnetic microsphcres by using standard glycoproteins （ovalbumin, immunoglobulin G and catalase） and nonglycoproteins （human serum albumin, bovine hemoglobin, myoglobin, lysozyme, and ribonuclease A） as model proteins. Adsorption experiments, SDS-PAGE and mass spectrometry analysis demonstrated that the Fe3O4@PDA-PBA magnetic microspheres had much high binding capacity and selectivity for glycoproteins/glycopeptides compared to nonglycoproteins/nonglycopeptides. In addition, the practicability of the Fe3O4@PDA-PBA magnetic microspheres was further assessed by selective capture of glycoproteins from healthy hu- man serum. The good results demonstrated its potential in glycoproteome analysis.</description><subject>Binding</subject><subject>Catalase</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Core-shell structure</subject><subject>Diameters</subject><subject>Glycopeptides</subject><subject>Glycoproteins</subject><subject>IgG antibody</subject><subject>Iron oxides</subject><subject>Lysozyme</subject><subject>Mass spectrometry</subject><subject>Microspheres</subject><subject>Myoglobins</subject><subject>Ovalbumin</subject><subject>Ribonuclease A</subject><subject>SDS-PAGE</subject><subject>Selectivity</subject><subject>Serum albumin</subject><subject>人血清白蛋白</subject><subject>固定化</subject><subject>多巴胺</subject><subject>磁性微球</subject><subject>糖肽</subject><subject>糖蛋白</subject><subject>苯基硼酸</subject><issn>1674-7291</issn><issn>1869-1870</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMFuGyEURUdVKtVK_AHZoXZNC8MAw7KymraSpWSRrhEDb2yiGZgAqeT8RH45uLbSXdjAe9xzH9ymuabkKyVEfsuUdq3AhHaYt73A_EOzor1QmPaSXNSzkB2WraKfmnXOD6Quxkgr-ap5uYvTwcXFzD4AnsF5U8AhP89x8JN_NsXHgOKIlj2EwzTEFIO3yFjvUL2YzS5AqY3Z2xRzFSXIaIwJZZjAFv8XEITk7X6GUI4-u-lg45JiAR8yMsGdO7AU7yBfNR9HM2VYn_fL5s_Nj_vNL7y9_fl7832LbUdZwb1RrBfcEEKHwRlBHLEjE5ITOyg6AieK10oOogWnlHBSOlkR2ve8GwVjl82Xk299yuMT5KIf4lMKdaSuOfW8sv9U9KQ6fi4nGPWS_GzSQVOij9HrU_S6Rq-P0WtemfbE5KoNO0j_nd-DPp8H7WPYPVbubZIQnWKCqpa9AvyplTQ</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Zheng, Jiangnan</creator><creator>Lin, Zian</creator><creator>Zhang, Lan</creator><creator>Yang, Huanghao</creator><general>Science China Press</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>M2P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20150601</creationdate><title>Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides</title><author>Zheng, Jiangnan ; Lin, Zian ; Zhang, Lan ; Yang, Huanghao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-8a93865a001bbda60d0cf36750cb91fe50956757b62ed996d77d793818854f633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Binding</topic><topic>Catalase</topic><topic>Chemical synthesis</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Core-shell structure</topic><topic>Diameters</topic><topic>Glycopeptides</topic><topic>Glycoproteins</topic><topic>IgG antibody</topic><topic>Iron oxides</topic><topic>Lysozyme</topic><topic>Mass spectrometry</topic><topic>Microspheres</topic><topic>Myoglobins</topic><topic>Ovalbumin</topic><topic>Ribonuclease A</topic><topic>SDS-PAGE</topic><topic>Selectivity</topic><topic>Serum albumin</topic><topic>人血清白蛋白</topic><topic>固定化</topic><topic>多巴胺</topic><topic>磁性微球</topic><topic>糖肽</topic><topic>糖蛋白</topic><topic>苯基硼酸</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Jiangnan</creatorcontrib><creatorcontrib>Lin, Zian</creatorcontrib><creatorcontrib>Zhang, Lan</creatorcontrib><creatorcontrib>Yang, Huanghao</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Science Database (ProQuest)</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Science China. Chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Jiangnan</au><au>Lin, Zian</au><au>Zhang, Lan</au><au>Yang, Huanghao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides</atitle><jtitle>Science China. Chemistry</jtitle><stitle>Sci. China Chem</stitle><addtitle>SCIENCE CHINA Chemistry</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>58</volume><issue>6</issue><spage>1056</spage><epage>1064</epage><pages>1056-1064</pages><issn>1674-7291</issn><eissn>1869-1870</eissn><abstract>In this work, we demonstrate for the first time, a method to synthesize phenylboronic acid-Fe304@polydopamine （Fe3O4@ PDA-PBA） magnetic microspheres via the combination of mussel-inspired polydopamine coating and click chemistry. Uniform-size and core-shell structured Fe3O4@PDA-PBA magnetic microspheres with a core diameter of -240 nm and a shell thickness of -13 nm were obtained as identified by the characterization of the morphology, structure and composition of the synthesized microspheres. We evaluated the selectivity and binding capacity of the Fe3O4@PDA-PBA magnetic microsphcres by using standard glycoproteins （ovalbumin, immunoglobulin G and catalase） and nonglycoproteins （human serum albumin, bovine hemoglobin, myoglobin, lysozyme, and ribonuclease A） as model proteins. Adsorption experiments, SDS-PAGE and mass spectrometry analysis demonstrated that the Fe3O4@PDA-PBA magnetic microspheres had much high binding capacity and selectivity for glycoproteins/glycopeptides compared to nonglycoproteins/nonglycopeptides. In addition, the practicability of the Fe3O4@PDA-PBA magnetic microspheres was further assessed by selective capture of glycoproteins from healthy hu- man serum. The good results demonstrated its potential in glycoproteome analysis.</abstract><cop>Beijing</cop><pub>Science China Press</pub><doi>10.1007/s11426-014-5286-5</doi><tpages>9</tpages></addata></record> |
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| subjects | Binding Catalase Chemical synthesis Chemistry Chemistry and Materials Science Chemistry/Food Science Core-shell structure Diameters Glycopeptides Glycoproteins IgG antibody Iron oxides Lysozyme Mass spectrometry Microspheres Myoglobins Ovalbumin Ribonuclease A SDS-PAGE Selectivity Serum albumin 人血清白蛋白 固定化 多巴胺 磁性微球 糖肽 糖蛋白 苯基硼酸 |
| title | Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides |
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